2. Academic Validation
  3. Improved isolation and characterization procedure of sialylglycopeptide from egg yolk powder

Improved isolation and characterization procedure of sialylglycopeptide from egg yolk powder

  • Carbohydr Res. 2017 Nov 27;452:122-128. doi: 10.1016/j.carres.2017.10.001.
Lin Liu 1,  Anthony R Prudden 1,  Gerlof P Bosman 2,  Geert-Jan Boons 3
Affiliations

Affiliations

  • 1 Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA.
  • 2 Chemical Biology and Drug Discovery, Utrecht University, 3584 CG Utrecht, The Netherlands; Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CG Utrecht, The Netherlands.
  • 3 Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA; Department of Chemistry, University of Georgia, Athens, GA 30602, USA; Chemical Biology and Drug Discovery, Utrecht University, 3584 CG Utrecht, The Netherlands; Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CG Utrecht, The Netherlands; Bijvoet Center for Biomolecular Research, Utrecht University, 3584 CH Utrecht, The Netherlands. Electronic address: [email protected].
PMID: 29096185 DOI: 10.1016/j.carres.2017.10.001
Abstract

Sialylglycopeptide (SGP) is a complex bi-antennary N-glycan bearing a short peptide fragment that can be isolated from the yolk of hen eggs. This natural product has gained popularity as a starting material for the semi-synthesis of N-glycans. We have found that current isolation methods provide a Glycopeptide contaminated with several related structures, one being a Glycopeptide having a hexose directly attached to peptide backbone, most like through the hydroxyl containing side chain of the threonine moiety. Furthermore, current methods employ fresh egg yolks that need to be lyophilized and involve several tedious purification steps. Herein, we report a convenient method for the isolation of gram quantities of homogeneous SGP from commercially available egg yolk powder using solid/liquid extraction and HILIC-HPLC purification.

Keywords

Egg yolk powder; HILIC-HPLC; Isolation; N-glycan; Sialylglycopeptide.

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