Analysis of Chemical Variations between Crude and Salt-Processed Anemarrhenae rhizoma Using Ultra-High-Performance Liquid Chromatography-Mass Spectrometry Methods

Molecules. 2017 Dec 22;23(1):23. doi: 10.3390/molecules23010023.

De Ji ¹ ² ³, Xiaonan Su ⁴ ⁵ ⁶, Ziyan Huang ⁷ ⁸ ⁹, Lialin Su ¹⁰ ¹¹ ¹², Lin Li ¹³ ¹⁴ ¹⁵, Tulin Lu ¹⁶ ¹⁷ ¹⁸

Affiliations

1 College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
2 State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
3 Key Research Laboratory of Chinese Medicine Processing of Jiangsu Province, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
4 College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
5 State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
6 Key Research Laboratory of Chinese Medicine Processing of Jiangsu Province, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
7 College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
8 State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
9 Key Research Laboratory of Chinese Medicine Processing of Jiangsu Province, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
10 College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
11 State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
12 Key Research Laboratory of Chinese Medicine Processing of Jiangsu Province, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
13 College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
14 State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
15 Key Research Laboratory of Chinese Medicine Processing of Jiangsu Province, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
16 College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
17 State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].
18 Key Research Laboratory of Chinese Medicine Processing of Jiangsu Province, Nanjing University of Chinese Medicine, Nanjing 210023, China. [email protected].

PMID: 29271935 DOI: 10.3390/molecules23010023

Abstract

The present study was designed to systematically investigate the chemical profile differences between crude Anemarrhenae rhizoma (CAR) and salt-processed Anemarrhenae rhizoma (SAR). Ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS), coupled with multivariate statistical analysis was used for the discrimination of chemical profiles and the identification of the differentiation of the chemical constitutions of CAR and SAR. In addition, seven main constituents of CAR and SAR were simultaneously determined by ultra-high-performance liquid chromatography-quadrupole mass spectrometry (UHPLC-MS) for analyzing the content variations. A total of 24 components were found to be the main contributors to the significant difference between CAR and SAR. The structures of the marker compounds were identified based on their chromatographic behaviors, intact precursor ions, and characteristic MS fragmentation patterns. The potential structural transformation mechanism of furostanol saponins during salt processing was explored. The results may provide a scientific foundation for deeply elucidating the processing mechanism of Anemarrhenae rhizoma.

Keywords

Anemarrhenae rhizoma; UPLC–QTOF-MS; chemical profile; furostanol saponins; salt processing.

Products

Cat. No.

Product Name

Description

Target

Research Area
HY-N18263

(25R)-Timosaponin BII

Furostanol Saponin

Others

Others

Name
Email *

	Sorry, but the email address you supplied was invalid.