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  3. Computational insights into β-site amyloid precursor protein enzyme 1 (BACE1) inhibition by tanshinones and salvianolic acids from Salvia miltiorrhiza via molecular docking simulations

Computational insights into β-site amyloid precursor protein enzyme 1 (BACE1) inhibition by tanshinones and salvianolic acids from Salvia miltiorrhiza via molecular docking simulations

  • Comput Biol Chem. 2018 Jun;74:273-285. doi: 10.1016/j.compbiolchem.2018.04.008.
Ting Yu 1,  Pradeep Paudel 1,  Su Hui Seong 1,  Jeong Ah Kim 2,  Hyun Ah Jung 3,  Jae Sue Choi 4
Affiliations

Affiliations

  • 1 Department of Food and Life Science, Pukyong National University, Busan, 48513, Republic of Korea.
  • 2 College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
  • 3 Department of Food Science and Human Nutrition, Chonbuk National University, Jeonju, 54896, Republic of Korea. Electronic address: [email protected].
  • 4 Department of Food and Life Science, Pukyong National University, Busan, 48513, Republic of Korea. Electronic address: [email protected].
PMID: 29679864 DOI: 10.1016/j.compbiolchem.2018.04.008
Abstract

The rhizome of Salvia miltiorrhiza has emerged as a rich source of natural therapeutic agents, and its several compounds are supposed to exhibit favorable effects on Alzheimer's disease (AD). The present work investigate the anti-AD potentials of 12 tanshinones, three salvianolic acids and three caffeic acid derivatives from S. miltiorrhiza via the inhibition of β-site amyloid precursor protein cleaving Enzyme 1 (BACE1). Among the tested compounds, deoxyneocryptotanshinone (1), salvianolic acid A (13) and salvianolic acid C (15) displayed good inhibitory effect on BACE1 with IC50 values of 11.53 ± 1.13, 13.01 ± 0.32 and 9.18 ± 0.03 μM, respectively. Besides this, Enzyme kinetic analysis on BACE1 revealed 13, a competitive type inhibitor while 1 and 15 showed mixed-type inhibition. Furthermore, molecular docking simulation displayed negative binding energies (AutoDock 4.2.6 = -10.0 to -7.1 kcal/mol) of 1, 13, and 15 for BACE1, indicating these compounds bound tightly to the active site of the Enzyme with low energy and high affinity. The results of the present study clearly demonstrate that S. miltiorrhiza and its constituents have potential anti-AD activity and can be used as a therapeutic agent for the treatment of AD.

Keywords

BACE1; Cholinesterase; Kinetics; Molecular docking; Salvia miltiorrhiza; Tanshionones.

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