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  3. A photoelectrochemical aptasensor constructed with core-shell CuS-TiO2 heterostructure for detection of microcystin-LR

A photoelectrochemical aptasensor constructed with core-shell CuS-TiO2 heterostructure for detection of microcystin-LR

  • Biosens Bioelectron. 2018 Oct 15;117:224-231. doi: 10.1016/j.bios.2018.06.007.
Yunfei Tang 1,  Yun Chai 1,  Xiaoqiang Liu 2,  Lele Li 1,  Liwei Yang 1,  Peipei Liu 1,  Yanmei Zhou 1,  Huangxian Ju 3,  Yunzhi Cheng 4
Affiliations

Affiliations

  • 1 Henan Joint International Research Laboratory of environmental pollution control materials, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan Province 475004, PR China.
  • 2 Henan Joint International Research Laboratory of environmental pollution control materials, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan Province 475004, PR China. Electronic address: [email protected].
  • 3 State Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210023, PR China. Electronic address: [email protected].
  • 4 Journal of Henan University (Medical Science), Henan University, Kaifeng, Henan Province 475004, PR China.
PMID: 29906770 DOI: 10.1016/j.bios.2018.06.007
Abstract

In this work, a CuS-TiO2 heterojunction composite was prepared by dispersedly depositing CuS nanoparticles on TiO2 nanospheres surface with a hydrothermal method, and was then used to construct a photoelectrochemical (PEC) aptasensor for sensitive detection of microcystin-LR (MC-LR) in aquatic environment. The energy bands of CuS nanoparticles and spherical anatase TiO2 were well matched, which enhanced the photo-to-current conversion efficiency. The composite exhibited the enhanced visible LIGHT absorption, the improved separation of photo-generated charges, and the reduced self-aggregation of CuS nanoparticles, leading to the enhanced photocurrent response. The PEC aptasensor was constructed by immobilizing CuS-TiO2 composite on ITO electrode with chitosan film that further covalently bound aminated aptamer. After the target, microcystin-LR (MC-LR) as an analyte model, was captured by the aptamer on the aptasensor, it could be oxidized by the photo-generated hole to impede the electron-hole recombination and further amplify the photocurrent. The PEC aptasensor showed superior analytical performance for MC-LR with a linear range of 5.0 × 10-5 nM to 250 nM and a detection limit of 2.0 × 10-5 nM. The detection results with the aptasensor for practical water samples indicated its promising application in environmental monitoring.

Keywords

CuS nanoparticles; CuS-TiO(2) heterojunction; Microcystin-LR; Photoelectrochemical aptasensor; TiO(2) nanospheres.

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