2. Academic Validation
  3. Temporal Regulation of the Bacterial Metabolite Deoxycholate during Colonic Repair Is Critical for Crypt Regeneration

Temporal Regulation of the Bacterial Metabolite Deoxycholate during Colonic Repair Is Critical for Crypt Regeneration

  • Cell Host Microbe. 2018 Sep 12;24(3):353-363.e5. doi: 10.1016/j.chom.2018.07.019.
Umang Jain 1,  Chin-Wen Lai 1,  Shanshan Xiong 1,  Victoria M Goodwin 1,  Qiuhe Lu 1,  Brian D Muegge 1,  George P Christophi 2,  Kelli L VanDussen 1,  Bethany P Cummings 3,  Erick Young 4,  John Hambor 4,  Thaddeus S Stappenbeck 5
Affiliations

Affiliations

  • 1 Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • 2 Division of Gastroenterology, Department of Medicine, Washington University School of Medicine, St. Louis, MO, 63110 USA.
  • 3 Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
  • 4 Research Beyond Borders, Boehringer Ingelheim Pharmaceuticals, Ridgefield, CT 06877, USA.
  • 5 Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA. Electronic address: [email protected].
PMID: 30122655 DOI: 10.1016/j.chom.2018.07.019
Abstract

Colonic wound repair is an orchestrated process, beginning with barrier re-establishment and followed by wound channel formation and crypt regeneration. Elevated levels of prostaglandin E2 (PGE2) promote barrier re-establishment; however, we found that persistently elevated PGE2 hinders subsequent repair phases. The Bacterial metabolite deoxycholate (DCA) promotes transition through repair phases via PGE2 regulation. During barrier re-establishment, DCA levels are locally diminished in the wound, allowing enhanced PGE2 production and barrier re-establishment. However, during transition to the wound channel formation phase, DCA levels increase to inhibit PGE2 production and promote crypt regeneration. Altering DCA levels via Antibiotic treatment enhances PGE2 levels but impairs wound repair, which is rescued with DCA treatment. DCA acts via its receptor, farnesoid X receptor, to inhibit the Enzyme cPLA2 required for PGE2 synthesis. Thus, colonic wound repair requires temporally regulated signals from microbial metabolites to coordinate host-associated signaling cascades. VIDEO ABSTRACT.

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