2. Academic Validation
  3. TROY interacts with RKIP to promote glioma development

TROY interacts with RKIP to promote glioma development

  • Oncogene. 2019 Feb;38(9):1544-1559. doi: 10.1038/s41388-018-0503-x.
Xiujie Liu 1,  Yinghui Bao 2,  Wei Meng 3,  Ping Yang 4,  Yi An 5,  Jie Ma 3,  Yujie Tang 6,  Zhigang Liu 7,  Yan Lu 8,  Jianfeng Zhou 8,  Yong Zhang 8,  Jifeng Feng 8,  Xiaofei Gao 8,  Zhida Su 8,  Yingyan Pu 8,  Cheng He 9
Affiliations

Affiliations

  • 1 Institute of Neuroscience, Key Laboratory of Molecular Neurobiology of Ministry of Education and the Collaborative Innovation Center for Brain Science, Second Military Medical University, Shanghai, 200433, China. [email protected].
  • 2 Department of Neurosurgery, Ren Ji Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, 200127, China.
  • 3 Department of Pediatric Neurosurgery, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai, 200092, P.R. China.
  • 4 Instrumental Analysis Center, School of Pharmacy, Fudan University, Shanghai, 201203, China.
  • 5 Department of Therapeutic Oncology, Yale University School of Medicine, New Haven, CT, USA.
  • 6 Key Laboratory of Cell Differentiation and Apoptosis of National Ministry of Education, Department of Pathophysiology, Shanghai Jiao Tong University, School of Medicine, 280 South Chongqing Road, Shanghai, 200025, P.R. China.
  • 7 Department of Radiation Oncology; Phase 1 Clinical Trial Ward, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, Guangdong Province, 519001, China.
  • 8 Institute of Neuroscience, Key Laboratory of Molecular Neurobiology of Ministry of Education and the Collaborative Innovation Center for Brain Science, Second Military Medical University, Shanghai, 200433, China.
  • 9 Institute of Neuroscience, Key Laboratory of Molecular Neurobiology of Ministry of Education and the Collaborative Innovation Center for Brain Science, Second Military Medical University, Shanghai, 200433, China. [email protected].
PMID: 30337686 DOI: 10.1038/s41388-018-0503-x
Abstract

TROY is a component of the Nogo receptor complex and plays the key role in neuronal survival, migration, and differentiation. Here, we show the up-regulation of TROY in human glioma tissues and cells. Inhibition of TROY expression slowed glioma development in vivo and in vitro. Raf kinase inhibitor (RKIP) was found to interact with TROY. The physical interaction of TROY/RKIP was confirmed via co-immunoprecipitation (co-IP) assays. Furthermore, we found that the TROY/RKIP interaction was enhanced by fetal bovine serum (FBS) exposure, and TROY knockdown also led to down-regulation of NF-κB. Finally, disruption of the TROY/RKIP interaction using the TAT-TROY (234-371 aa) protein reduced the glioma development in xenografted mice. This suggests the TROY/RKIP interaction is a potential target for therapy of gliomas.

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