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  3. Adenosine and adenosine-5'-monophosphate ingestion ameliorates abnormal glucose metabolism in mice fed a high-fat diet

Adenosine and adenosine-5'-monophosphate ingestion ameliorates abnormal glucose metabolism in mice fed a high-fat diet

  • BMC Complement Altern Med. 2018 Nov 14;18(1):304. doi: 10.1186/s12906-018-2367-6.
Ardiansyah 1 2,  Yuto Inagawa 3,  Takuya Koseki 4,  Afifah Zahra Agista 3,  Ikuo Ikeda 5,  Tomoko Goto 3,  Michio Komai 3,  Hitoshi Shirakawa 6 7
Affiliations

Affiliations

  • 1 Laboratory of Nutrition, Graduate School of Agricultural Science, Tohoku University, Sendai, 980-0845, Japan. [email protected].
  • 2 Department of Food Science and Technology, Universitas Bakrie, Jakarta, 12920, Indonesia. [email protected].
  • 3 Laboratory of Nutrition, Graduate School of Agricultural Science, Tohoku University, Sendai, 980-0845, Japan.
  • 4 Faculty of Agriculture, Yamagata University, Tsuruoka, 997-8555, Japan.
  • 5 Laboratory of Food and Biomolecular Science, Graduate School of Agricultural Science, Tohoku University, Sendai, 980-0845, Japan.
  • 6 Laboratory of Nutrition, Graduate School of Agricultural Science, Tohoku University, Sendai, 980-0845, Japan. [email protected].
  • 7 International Education and Research Center for Food Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, 980-0845, Japan. [email protected].
PMID: 30428888 DOI: 10.1186/s12906-018-2367-6
Abstract

Background: We have previously reported that ingestion of adenosine (ADN) and adenosine-5'-monophosphate (AMP) improves abnormal glucose metabolism in the stroke-prone spontaneously hypertensive rat model of non-obesity-associated Insulin resistance. In this study, we investigated the effect of ADN and AMP ingestion on glucose metabolism in mice with high-fat diet-induced obesity.

Methods: Seven-week-old C57BL/6 J mice were administered distilled water (as a control), 10 mg/L ADN, or 13 mg/L AMP via their drinking water for 14 or 25 weeks, during which they were fed a high-fat diet. Oral glucose tolerance test (OGTT) was conducted on 21-week-old mice fasted for 16 h. Insulin tolerance test (ITT) was performed on 22-week-old mice fasted for 3 h. Blood and muscle were collected for further analysis of serum parameters, gene and protein expression levels, respectively.

Results: Glucose metabolism in the ADN and AMP groups was significantly improved compared with the control. OGTT and ITT showed that ADN and AMP groups lower than control group. Furthermore, phosphorylation of AMP-activated protein kinase (AMPK) and mRNA levels of genes involved in lipid oxidation were enhanced in the skeletal muscle of ADN- and AMP-treated mice.

Conclusion: These results indicate that ingestion of ADN or AMP induces activation of AMPK in skeletal muscle and mitigates Insulin resistance in mice with high-fat diet-induced diabetes.

Keywords

Adenosine; Adenosine-5′-monophosphate; Glucose metabolism; High-fat diet.

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