2. Academic Validation
  3. Bioelectrocatalytic performance of d-fructose dehydrogenase

Bioelectrocatalytic performance of d-fructose dehydrogenase

  • Bioelectrochemistry. 2019 Oct:129:1-9. doi: 10.1016/j.bioelechem.2019.04.024.
Taiki Adachi 1,  Yuya Kaida 1,  Yuki Kitazumi 1,  Osamu Shirai 1,  Kenji Kano 2
Affiliations

Affiliations

  • 1 Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto 606-8502, Japan.
  • 2 Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto 606-8502, Japan. Electronic address: [email protected].
PMID: 31063949 DOI: 10.1016/j.bioelechem.2019.04.024
Abstract

This review summarizes the bioelectrocatalytic properties of d-fructose dehydrogenase (FDH), while taking into consideration its enzymatic characteristics. FDH is a membrane-bound flavohemo-protein with a molecular mass of 138 kDa, and it catalyzes the oxidation of d-fructose to 5-keto-d-fructose. The characteristic feature of FDH is its strong direct-electron-transfer (DET)-type bioelectrocatalytic activity. The pathway of the DET-type reaction is discussed. An overview of the application of FDH-based bioelectrocatalysis to biosensors and biofuel cells is also presented, and the benefits and problems associated with it are extensively discussed.

Keywords

Biofuel cells; Biosensors; Electron transfer pathway; Fructose dehydrogenase; Protein engineering.

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