2. Academic Validation
  3. Antimicrobial peptides Pep19-2.5 and Pep19-4LF inhibit Streptococcus mutans growth and biofilm formation

Antimicrobial peptides Pep19-2.5 and Pep19-4LF inhibit Streptococcus mutans growth and biofilm formation

  • Microb Pathog. 2019 Aug;133:103546. doi: 10.1016/j.micpath.2019.103546.
Hanen Jannadi 1,  Wilmar Correa 2,  Ze Zhang 3,  Klaus Brandenburg 4,  Ridha Oueslati 5,  Mahmoud Rouabhia 6
Affiliations

Affiliations

  • 1 Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, 2420, rue de la Terrasse, Québec, G1V 0A6, QC, Canada; Unité IMEC, Faculté des Sciences de Bizerte, Unversité de Carthage, Tunisia.
  • 2 Forschungszentrum Borstel, Leibniz-Zentrum für Medizin und Biowissenschaften, Parkallee, D-23845, Borstel, Germany.
  • 3 Département de Chirurgie, Faculté de Médecine, Université Laval, and L'Axe Médecine régénératrice, Centre de recherche du CHU de Québec, Québec, G1L 3L5, Canada.
  • 4 Brandenburg Antiinfektiva GmbH, c/o Forschungszentrum Borstel, D-23845, Borstel, Germany.
  • 5 Unité IMEC, Faculté des Sciences de Bizerte, Unversité de Carthage, Tunisia.
  • 6 Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, 2420, rue de la Terrasse, Québec, G1V 0A6, QC, Canada. Electronic address: [email protected].
PMID: 31112769 DOI: 10.1016/j.micpath.2019.103546
Abstract

With this study, we investigated the effect of synthetic antimicrobial Peptides Pep19-2.5 and Pep194LF alone or in combination with Antibiotics on S. mutans growth and biofilm formation/disruption. We also examined the cytotoxic effect of each peptide on monocytes. S. mutans was cultured in the presence of different concentrations of each peptide. We showed that Pep19-2.5 and Pep19-4LF were able to significantly (p ≤ 0.01) inhibit the growth of S. mutans. The synthetic Peptides also decreased biofilm formation by S. mutans. Furthermore, both Peptides reduced the viability of S. mutans in already formed biofilms. The combination of each peptide with Antibiotics (penicillin/streptomycin, P/S) produced additive interactions which inhibited S. mutans growth and biofilm formation. Pep19-2.5 and Pep19-4LF were nontoxic, as they did not decrease monocyte viability and did not increase the Lactate Dehydrogenase activity of the exposed cells. In conclusion, synthetic Peptides Pep19-2.5 and Pep19-4LF did inhibit S. mutans growth and its capacity to form biofilm. Both Peptides were found to be nontoxic to monocytes. These data provide new insight into the efficacy of synthetic Peptides Pep19-2.5 and Pep19-4LF against S. mutans. These Peptides may thus be useful in controlling the adverse effects of this cariogenic bacterium in human.

Keywords

Antimicrobial peptides; Bacterial growth; Biofilms; Monocytes; S. mutans.

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