2. Academic Validation
  3. Co-production of chondroitin sulfate and peptide from liquefied chicken sternal cartilage by hot-pressure

Co-production of chondroitin sulfate and peptide from liquefied chicken sternal cartilage by hot-pressure

  • Carbohydr Polym. 2019 Oct 15;222:115015. doi: 10.1016/j.carbpol.2019.115015.
Qingshan Shen 1,  Chunhui Zhang 2,  Wei Jia 3,  Xiaojie Qin 3,  Zhenkun Cui 4,  Haizhen Mo 4,  Aurore Richel 5
Affiliations

Affiliations

  • 1 Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China; Department of Food Science, Henan Institute of Science and Technology, Xinxiang, 453003, China; University of Liege-Gembloux Agro-Bio Tech, Laboratory of Biomass and Green Technologies, Passage des déportés 2, B-5030, Gembloux, Belgium.
  • 2 Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Electronic address: [email protected].
  • 3 Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
  • 4 Department of Food Science, Henan Institute of Science and Technology, Xinxiang, 453003, China.
  • 5 University of Liege-Gembloux Agro-Bio Tech, Laboratory of Biomass and Green Technologies, Passage des déportés 2, B-5030, Gembloux, Belgium.
PMID: 31320074 DOI: 10.1016/j.carbpol.2019.115015
Abstract

Co-production of chondroitin sulfate (CS) and Peptides was realized from the liquid fraction of chicken sternal cartilage subjected to hot-pressure (HP) by membrane combination separation technology. Cartilage was liquefied via the HP treatment at 110 °C (0.07 MPa) and 120 °C (0.1 MPa) for 0.5 - 2.5 h, respectively. The optimized co-production procedure was as follows: enzymolysis temperature, 61.2 °C; the Enzyme ratio of trypsin and papain, 1.3:1 (W/W); enzymolysis time ratio, 2:2 (h/h), under which the highest yields of CS and Peptides were 18.85% and 67.99%, and the recoveries were 93.63% and 92.69%. The average molecular weight of CS sample was 67.79 kDa. CS sample was confirmed using agarose-gel electrophoresis, and the structure was analyzed by Fourier transform infrared spectroscopy, chromatography and nuclear magnetic resonance. Taken together, HP can be as a pretreatment method to liquefy cartilage for the industrial co-production of CS and Peptides with eco-friendly.

Keywords

Chondroitin sulfate; Co-production; Hot-pressure; Liquefaction; Peptides.

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