2. Academic Validation
  3. Fangchinoline derivatives induce cell cycle arrest and apoptosis in human leukemia cell lines via suppression of the PI3K/AKT and MAPK signaling pathway

Fangchinoline derivatives induce cell cycle arrest and apoptosis in human leukemia cell lines via suppression of the PI3K/AKT and MAPK signaling pathway

  • Eur J Med Chem. 2020 Jan 15;186:111898. doi: 10.1016/j.ejmech.2019.111898.
Jin Yang 1,  Shengcao Hu 1,  Chunlin Wang 2,  Junrong Song 2,  Chao Chen 2,  Yanhua Fan 3,  Yaacov Ben-David 4,  Weidong Pan 5
Affiliations

Affiliations

  • 1 College of Pharmacy, Zunyi Medical University, Zunyi, 563000, China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences/Guizhou Provincial Engineering Research Center for Natural Drugs, Guiyang, 550014, China.
  • 2 State Key Laboratory for Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, 550014, China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences/Guizhou Provincial Engineering Research Center for Natural Drugs, Guiyang, 550014, China.
  • 3 State Key Laboratory for Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, 550014, China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences/Guizhou Provincial Engineering Research Center for Natural Drugs, Guiyang, 550014, China. Electronic address: [email protected].
  • 4 State Key Laboratory for Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, 550014, China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences/Guizhou Provincial Engineering Research Center for Natural Drugs, Guiyang, 550014, China. Electronic address: [email protected].
  • 5 State Key Laboratory for Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, 550014, China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences/Guizhou Provincial Engineering Research Center for Natural Drugs, Guiyang, 550014, China. Electronic address: [email protected].
PMID: 31784186 DOI: 10.1016/j.ejmech.2019.111898
Abstract

Fangchinoline, a bisbenzylisoquinoline alkaloid extracted from Stephania tetrandra S. Moore, is known to exert anti-cancer activity. A series of new fangchinoline derivatives have been synthesized and evaluated for their anti-cancer activity. In cell viability assay, these fangchinoline derivatives displayed higher proliferation inhibitory activity on leukemia and breast Cancer cell lines than the parental compound. Among them, 3e exhibited strongest cell growth inhibitory activity in a dose- and time-dependent manner on leukemia cell line HEL through induction of G0/G1 cell cycle arrest and Apoptosis. Treatment of HEL cells with 3e also resulted in significant suppression of the MAPK and PI3K/Akt pathway as well as c-Myc downregulation, which may responsible for induction of Apoptosis and cell cycle arrest. In docking analysis, high affinity interaction between 3e and Akt1 was responsible for drastic kinase inhibition by the compound. This derivative compound may be useful as a potent anti-cancer agent for treatment of leukemia.

Keywords

Apoptosis; Cell cycle arrest; Fangchinoline; Hit compound; Leukemia; PI3K/AKT pathway; c-MYC.

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