1. Academic Validation
  2. In vitro impact of bisphenol A on maturation and function of monocyte-derived dendritic cells in patients with primary Sjögren's syndrome

In vitro impact of bisphenol A on maturation and function of monocyte-derived dendritic cells in patients with primary Sjögren's syndrome

  • Immunopharmacol Immunotoxicol. 2020 Feb;42(1):28-36. doi: 10.1080/08923973.2019.1706554.
Jing Wang 1 Chunhui She 1 Zhiyuan Li 2 Ning Tang 1 Lishan Xu 1 Zhaoyang Liu 1 Bin Liu 1
Affiliations

Affiliations

  • 1 Department of Rheumatology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.
  • 2 Department of Otolaryngology-Head and Neck Surgery, Key Laboratory, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.
Abstract

Background: Epidemiological studies have shown that environmental factors accelerate the progress of primary Sjögren's syndrome (pSS). Bisphenol A (BPA), a classic endocrine disrupting chemical, affects the immune system. However, the impact of BPA on pSS has not yet been reported. The present study aimed to evaluate the potential relationship between BPA, Estrogen Receptor (ER), and pSS.Methods: We studied the impact of BPA on monocyte-derived dendritic cells (moDCs) from pSS patients and age-matched healthy controls (HCs). Morphological effects were observed under inverted microscope. Surface markers were analyzed by flow cytometry. ER and cytokine profiles were assessed using real-time polymerase chain reaction. The ability of moDCs to stimulate CD4+ T cells activation was assessed by mixed lymphocyte reaction (MLR).Results: moDCs from both pSS patients and HCs expressed ERα as well as ERβ. After BPA-exposure, expression of ERα increased significantly in pSS patients, while that of ERβ remained unchanged. moDCs from BPA-exposed pSS patients showed irregular morphology and reduction in cell aggregation. BPA increased HLA-DR on moDCs of pSS patients via ERα, and promoted the secretion of IL6 and IL12. When co-cultured with BPA-treated moDCs, cytokines (IFN-γ, IL4, IL17, IL10) and transcription factors (T-bet, Gata3, RoR-γt, Foxp3) of CD4+ T cells showed imbalance of Th1/Th2/Th17/Treg polarization, with Th1 and Th17 dominating.Conclusions: BPA altered the function of moDCs through ERα, including antigen capture, secretion of inflammatory factors, and ability to stimulate T cells, as well as accelerated the progression and further deterioration of pSS.

Keywords

CD4+ T cells; Primary Sjögren's syndrome; bisphenol A; dendritic cells; estrogen receptor α.

Figures
Products
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  • HY-D0938
    99.01%, Cell Proliferation Fluorescent Probe