2. Academic Validation
  3. Molecular Behavior of HMGB1 in the Cochlea Following Noise Exposure and in vitro

Molecular Behavior of HMGB1 in the Cochlea Following Noise Exposure and in vitro

  • Front Cell Dev Biol. 2021 Feb 25:9:642946. doi: 10.3389/fcell.2021.642946.
Lili Xiao 1 2 3,  Yan Sun 4,  Chengqi Liu 1 2 3,  Zhong Zheng 1 2 3,  Ying Shen 1 2 3,  Liang Xia 1 2 3,  Guang Yang 1 2 3,  Yanmei Feng 1 2 3
Affiliations

Affiliations

  • 1 Department of Otolaryngology-Head and Neck Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
  • 2 Otolaryngology Institute of Shanghai Jiao Tong University, Shanghai, China.
  • 3 Shanghai Key Laboratory of Sleep Disordered Breathing, Shanghai, China.
  • 4 Zhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Zhangjiagang, China.
PMID: 33732708 DOI: 10.3389/fcell.2021.642946
Abstract

Noise-induced hearing loss (NIHL) is characterized by cellular damage to the inner ear, which is exacerbated by inflammation. High-mobility group box 1 (HMGB1), a representative damage-associated molecular pattern (DAMP), acts as a mediator of inflammation or an intercellular messenger according to its cellular localization. Blocking or regulating HMGB1 offers an attractive approach in ameliorating NIHL. However, the precise therapeutic intervention must be based on a deeper understanding of its dynamic molecular distribution and function in cochlear pathogenesis after acoustic trauma. Here, we have presented the spatiotemporal dynamics of the expression of HMGB1, exhibiting distribution variability in specific cochlear regions and cells following noise exposure. After gene manipulation, we further investigated the characteristics of cellular HMGB1 in HEI-OC1 cells. The higher cell viability observed in the HMGB1 knocked-down group after stimulation with H2O2 indicated the possible negative effect of HMGB1 on cellular lifespan. In conclusion, this study demonstrated that HMGB1 is involved in NIHL pathogenesis and its Molecular Biology has essential and subtle influences, preserving a translational potential for pharmacological intervention.

Keywords

cochlea; hearing loss; high-mobility group box 1; noise; spatiotemporal distribution.

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