2. Academic Validation
  3. REV-ERBs negatively regulate mineralization of the cementoblasts

REV-ERBs negatively regulate mineralization of the cementoblasts

  • Biochem Biophys Res Commun. 2022 Jan 8:587:9-15. doi: 10.1016/j.bbrc.2021.11.051.
Liangliang Fu 1,  Min Wang 1,  Guixin Zhu 1,  Zifan Zhao 2,  Huifang Sun 1,  Zhengguo Cao 3,  Haibin Xia 4
Affiliations

Affiliations

  • 1 The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China; Department of Oral Implantology, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
  • 2 Center of Digital Dentistry, Peking University School and Hospital of Stomatology, Beijing, PR China.
  • 3 The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
  • 4 The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China; Department of Oral Implantology, School and Hospital of Stomatology, Wuhan University, Wuhan, China. Electronic address: [email protected].
PMID: 34861472 DOI: 10.1016/j.bbrc.2021.11.051
Abstract

Objective: The role of circadian clock in cementogenesis is unclear. This study examines the role of REV-ERBs, one of circadian clock proteins, in proliferation, migration and mineralization of cementoblasts to fill the gap in knowledge.

Methods: Expression pattern of REV-ERBα in cementoblasts was investigated in vivo and in vitro. CCK-8 assay, scratch wound healing assay, Alkaline Phosphatase (ALP) and alizarin red S (ARS) staining were performed to evaluate the effects of REV-ERBs activation by SR9009 on proliferation, migration and mineralization of OCCM-30, an immortalized cementoblast cell line. Furthermore, mineralization related markers including osterix (OSX), ALP, bone sialoprotein (BSP) and osteocalcin (OCN) were evaluated.

Results: Strong expression of REV-ERBα was found in cellular cementum around tooth apex. Rev-erbα mRNA oscillated periodically in OCCM-30 and declined after mineralization induction. REV-ERBs activation by SR9009 inhibited proliferation but promoted migration of OCCM-30 in vitro. Results of ALP and ARS staining suggested that REV-ERBs activation negatively regulated mineralization of OCCM-30. Mechanically, REV-ERBs activation attenuated the expression of OSX and its downstream targets including ALP, BSP and OCN.

Conclusions: REV-ERBs are involved in cementogenesis and negatively regulate mineralization of cementoblasts via inhibiting OSX expression. Our study provides a potential target regarding periodontal and cementum regeneration.

Keywords

Cementoblasts; Mineralization; REV-ERBs; Regeneration; SR9009.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-16989
    99.46%, REV-ERB Agonist