2. Academic Validation
  3. Antibacterial, Anticandidal, and Antibiofilm Potential of Fenchone: In Vitro, Molecular Docking and In Silico/ADMET Study

Antibacterial, Anticandidal, and Antibiofilm Potential of Fenchone: In Vitro, Molecular Docking and In Silico/ADMET Study

  • Plants (Basel). 2022 Sep 14;11(18):2395. doi: 10.3390/plants11182395.
Wasim Ahmad 1,  Mohammad Azam Ansari 2,  Mohammad Yusuf 3,  Mohd Amir 4,  Shadma Wahab 5,  Prawez Alam 6,  Mohammad N Alomary 7,  Abdulrahman A Alhuwayri 8,  Maria Khan 9,  Abuzer Ali 10,  Musarrat Husain Warsi 11,  Kamran Ashraf 12,  Maksood Ali 13
Affiliations

Affiliations

  • 1 Department of Pharmacy, Mohammed Al-Mana College for Medical Sciences, Dammam 34222, Saudi Arabia.
  • 2 Department of Epidemic Disease Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia.
  • 3 Department of Clinical Pharmacy, College of Pharmacy, Taif University, Taif 21944, Saudi Arabia.
  • 4 Department of Natural Products and Alternative Medicine, College of Clinical Pharmacy, Imam Abdul Rahman bin Faisal University, Dammam 31441, Saudi Arabia.
  • 5 Department of Pharmacognosy, College of Pharmacy, King Khalid University, Abha 61421, Saudi Arabia.
  • 6 Department of Pharmacognosy, College of Pharmacy, Prince-Sattam Bin-Abdulaziz University, Al-Kharj 11942, Saudi Arabia.
  • 7 National Centre for Biotechnology, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi Arabia.
  • 8 Maternity and Children Hospital, Buraydah 51452, Saudi Arabia.
  • 9 Department of Pharmacognosy, R.V. Northland Institute, Dadri 203207, India.
  • 10 Department of Pharmacognosy, College of Pharmacy, Taif University, Taif 21944, Saudi Arabia.
  • 11 Department of Pharmaceutics and Industrial Pharmacy, College of Pharmacy, Taif University, Taif 21944, Saudi Arabia.
  • 12 Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Cawangan Selangor, Kampus Puncak Alam, Bandar Puncak Alam 42300, Selangor Darul Ehsan, Malaysia.
  • 13 Department of Pharmacognosy, Orlean College of Pharmacy 42, Knowledge Park-III, Greater Noida 201308, India.
PMID: 36145798 DOI: 10.3390/plants11182395
Abstract

The aim of the present study is to investigate the effective antimicrobial and antibiofilm properties of fenchone, a biologically active bicyclic monoterpene, against infections caused by bacteria and Candida spp. The interactions between fenchone and three distinct proteins from Escherichia coli (β-ketoacyl acyl carrier protein synthase), Candida albicans (1, 3-β−D-glucan synthase), and Pseudomonas aeruginosa (Anthranilate-CoA Ligase) were predicted using molecular docking and in silico/ADMET methods. Further, to validate the in-silico prediction, the Antibacterial and Antifungal potential of fenchone was evaluated against E. coli, P. aeruginosa, and C. albicans by determining minimum inhibitory concentration (MIC), minimum Bacterial concentration (MBC), and minimum fungicidal concentration (MFC). The lowest MIC/MBC values of fenchone against E. coli and P. aeruginosa obtained was 8.3 ± 3.6/25 ± 0.0 and 266.6 ± 115.4/533.3 ± 230.9 mg/mL, respectively, whereas the MIC/MFC value for C. albicans was found to be 41.6 ± 14.4/83.3 ± 28.8 mg/mL. It was observed that fenchone has a significant effect on antimicrobial activity (p < 0.05). Our findings demonstrated that fenchone at 1 mg/mL significantly reduced the production of biofilm (p < 0.001) in E. coli, P. aeruginosa, and C. albicans by 70.03, 64.72, and 61.71%, respectively, in a dose-dependent manner when compared to control. Based on these results, it has been suggested that the essential oil from Plants can be a great source of pharmaceutical ingredients for developing new antimicrobial drugs.

Keywords

antimicrobial activity; biofilm; essential oil; fenchone; molecular docking.

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