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  3. Broccoli seed extract rich in polysaccharides and glucoraphanin ameliorates DSS-induced colitis via intestinal barrier protection and gut microbiota modulation in mice

Broccoli seed extract rich in polysaccharides and glucoraphanin ameliorates DSS-induced colitis via intestinal barrier protection and gut microbiota modulation in mice

  • J Sci Food Agric. 2022 Dec 9. doi: 10.1002/jsfa.12382.
Jiaying Wu 1 2,  Weiling Guo 1 2,  Shumao Cui 1 2,  Xin Tang 1 2,  Qiuxiang Zhang 1 2,  Wenwei Lu 1 2 3,  Yan Jin 4,  Jianxin Zhao 1 2,  Bingyong Mao 1 2,  Wei Chen 1 2 3
Affiliations

Affiliations

  • 1 State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China.
  • 2 School of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China.
  • 3 National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, 214122, P. R. China.
  • 4 The Affiliated Wuxi Second People's Hospital of Nanjing Medical University, Wuxi, 214002, P. R. China.
PMID: 36495024 DOI: 10.1002/jsfa.12382
Abstract

Background: Broccoli has received widespread attention due to its anti-inflammatory and antioxidant effects. The aim of this study was to explore the composition of broccoli seed extract (BSE) and its effect on colitis induced by dextran sulfate sodium (DSS).

Results: The main compositions of BSE are glucoraphanin and Polysaccharides composed of arabinose, galactose, glucose and mannose. The animal experiment suggested that BSE intervention effectively reversed body weight loss, suppressed the levels of proinflammatory interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), and elevated the levels of anti-inflammatory interleukin-10 (IL-10) and the activities of superoxide dismutase (SOD) and glutathione (GSH) in DSS-induced colitis mice. According to histopathologic and immunohistochemical analysis of colon tissue, BSE intervention may repair the intestinal barrier by upregulating the mRNA levels and expression of tight junction proteins (claudin-1, occludin and zonula occludens-1). The GC-MS analysis demonstrated that cecal short-chain fatty acids (SCFAs) in mice with BSE administration were significantly increased compared with the model group. Sulforaphane and sulforaphane-N-acetylcysteine were only detected in BSE group mice by UPLC-MS analysis. In addition, BSE intervention evidently increased the abundance of Alistipeds, Coriobacteriaceae UCG-002 and Bifidobacterium and decreased the abundance of Escheichia-Shinella, Lachnospiraceae Others, Parabacteroides, Ruminococcaceae Others and Turicibacter, which possibly promoted carbohydrate metabolism and SCFA production.

Conclusion: These results were intended to elucidate the effect of BSE on colitis and indicated that BSE, as a novel food ingredient, has great potential for colitis improvement. This article is protected by copyright. All rights reserved.

Keywords

Broccoli seed extract; Glucoraphanin; Gut microbiota; Intestinal barrier; Polysaccharides; Ulcerative colitis.

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