2. Academic Validation
  3. TRPC7 facilitates cell growth and migration by regulating intracellular Ca2+ mobilization in lung adenocarcinoma cells

TRPC7 facilitates cell growth and migration by regulating intracellular Ca2+ mobilization in lung adenocarcinoma cells

  • Oncol Lett. 2023 Jan 24;25(3):92. doi: 10.3892/ol.2023.13678.
Jui-Lin Liang 1 2,  Ming-Hsien Tsai 3,  Yi-Chun Hsieh 4,  Huei-Syuan Liu 4,  Shao-Wei Chen 5,  Yung-Yun Huang 6,  Li-Ching Lin 7,  Tsung-Fu Tsai 8,  Yun-Fang Liang 9,  Wen-Li Hsu 4 10
Affiliations

Affiliations

  • 1 Institute of Clinical Medicine, National Cheng Kung University College of Medicine, Tainan 701401, Taiwan, R.O.C.
  • 2 Department of Surgery, Chi-Mei Medical Center, Liouying, Tainan 73657, Taiwan, R.O.C.
  • 3 Department of Child Care, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan, R.O.C.
  • 4 Regenerative Medicine and Cell Therapy Research Center, Kaohsiung Medical University, Kaohsiung 80708, Taiwan, R.O.C.
  • 5 Department of Clinical Education and Training, Chung-Ho Memorial Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan, R.O.C.
  • 6 Department of Anesthesiology, Kaohsiung Veterans General Hospital, Kaohsiung 813414, Taiwan, R.O.C.
  • 7 Division of General Surgery, Chang Gung Memorial Hospital, New Taipei 33303, Taiwan, R.O.C.
  • 8 Department of Dermatology, Chang Gung Memorial Hospital, New Taipei 33303, Taiwan, R.O.C.
  • 9 School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan, R.O.C.
  • 10 Department of Dermatology, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80145, Taiwan, R.O.C.
PMID: 36817036 DOI: 10.3892/ol.2023.13678
Abstract

Transient receptor potential canonical 7 (TRPC7) has been reported to mediate aging-associated tumorigenesis, but the role of TRPC7 in Cancer malignancy is still unclear. TRPC7 is associated with tumor size in patients with lung adenocarcinoma and the present study further evaluated the underlying mechanism of TRPC7 in the regulation of Cancer progression. The clinicopathological role of TRPC7 was assessed using immunohistochemistry staining and the pathological mechanism of TRPC7 in lung adenocarcinoma cells was determined using cell cycle examination, invasion and calcium response assays, and immunoblot analysis. The results indicated that high TRPC7 expression was associated with a lower 5-year survival rate compared with low TRPC7 expression, which suggested that TRPC7 expression was inversely associated with overall survival in patients with lung adenocarcinoma. TRPC7 serves a pathological role by facilitating the enhancement of cell growth and migration with increased phosphorylation of CA2+/calmodulin-dependent protein kinase II, Akt and ERK. TRPC7 knockdown in lung adenocarcinoma cells restrained cell cycle progression and cell migration by interrupting the TRPC7-mediated CA2+ signaling-dependent Akt and MAPK signaling pathways. These findings demonstrated for the first time a role of oncogenic TRPC7 in the regulation of Cancer malignancy and could provide a novel therapeutic molecular target for patients with lung adenocarcinoma.

Keywords

Ca2+ signaling; cancer cell growth; cancer cell migration; lung adenocarcinoma; transient receptor potential canonical 7.

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