2. Academic Validation
  3. Metal-Induced Energy Transfer (MIET) for Live-Cell Imaging with Fluorescent Proteins

Metal-Induced Energy Transfer (MIET) for Live-Cell Imaging with Fluorescent Proteins

  • ACS Nano. 2023 May 9;17(9):8242-8251. doi: 10.1021/acsnano.2c12372.
Lara Hauke 1,  Sebastian Isbaner 1,  Arindam Ghosh 1,  Isabella Guido 2,  Laura Turco 2,  Alexey I Chizhik 1,  Ingo Gregor 1,  Narain Karedla 1,  Florian Rehfeldt 1,  Jörg Enderlein 1 3
Affiliations

Affiliations

  • 1 Third Institute of Physics - Biophysics, Georg August University, Friedrich-Hund-Platz 1, 37077 Göttingen, Germany.
  • 2 Max Planck Institute for Dynamics and Self-Organization, Am Faßberg 17, 37077 Göttingen, Germany.
  • 3 Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC), Universitätsmedizin Göttingen, Robert-Koch-Strasse 40, 37075 Göttingen, Germany.
PMID: 36995274 DOI: 10.1021/acsnano.2c12372
Abstract

Metal-induced energy transfer (MIET) imaging is an easy-to-implement super-resolution modality that achieves nanometer resolution along the optical axis of a microscope. Although its capability in numerous biological and biophysical studies has been demonstrated, its implementation for live-cell imaging with fluorescent proteins is still lacking. Here, we present its applicability and capabilities for live-cell imaging with fluorescent proteins in diverse cell types (adult human stem cells, human osteo-sarcoma cells, and Dictyostelium discoideum cells), and with various fluorescent proteins (GFP, mScarlet, RFP, YPet). We show that MIET imaging achieves nanometer axial mapping of living cellular and subcellular components across multiple time scales, from a few milliseconds to hours, with negligible phototoxic effects.

Keywords

axial resolution; fluorescent proteins; live-cell imaging; metal-induced energy transfer; super-resolution microscopy.

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