DOT1L Epigenetically Regulates Autophagy and Mitochondria Fusion in Cell Lines of Renal Cancer

Objectives: DOT1L, a histone methylase, is overexpression in renal cell Cancer. However, the role and detailed molecular mechanism of DOT1L involved in renal Cancer development remain unknown.

Methods: The inhibition of DOT1L was used by SGC0946 and short hairpin RNA silencing. Monodansylcadaverine staining and transmission electron microscope were performed to detect Autophagy changes as a result of the inhibition of DOT1L. MitoTracker Red assay was used to analyze mitochondrial morphology. The Autophagy markers and mitochondria-related proteins were analyzed by Western blot, qPCR, or immunofluorescence. ChIP assay was performed to demonstrate H3K79me2 is involved in the direct regulation of Farnesoid X receptor transcription.

Results: DOT1L inhibition increased Autophagy activity and promoted mito chondria fusion in cell lines of renal Cancer. Inhibition of DOT1L upregulated levels of LC3α/β, P62, MFN1, and MFN2, which contributed to Autophagy activity or mitochondria fusion. DOT1L knockdown showed a similar the above process. DOT1L inhibition or silencing resulted in AMP-activated protein kinase activation and mammalian target of rapamycin inhibition. Mechanistically, the DOT1L Inhibitor and its short hairpin RNAs decreased the expression of Farnesoid X receptor in a histone methylase-dependent manner.

Conclusion: We revealed the essential role of Farnesoid X receptor in regulating DOT1L-induced Autophagy and mitochondrial fission through the AMP-activated protein kinase/mammalian target of rapamycin pathway in cell lines of renal Cancer, which may provide new insights into the pathogenesis of renal cell Cancer.

DOT1L; FXR; autophagy; mitochondria fusion; renal cancer.