2. Academic Validation
  3. Novel mandelic acid derivatives suppress virulence of Ralstonia solanacearum via type III secretion system

Novel mandelic acid derivatives suppress virulence of Ralstonia solanacearum via type III secretion system

  • Pest Manag Sci. 2023 Nov;79(11):4626-4634. doi: 10.1002/ps.7664.
Qiao-Qiao Guo 1,  Yu-Zhen Li 1,  Hua-Bin Shi 2,  Ao-Yun Yi 1,  Xiao-Li Xu 3,  Hai-Hong Wang 4,  Xin Deng 5 6,  Zhi-Bing Wu 2,  Zi-Ning Cui 1
Affiliations

Affiliations

  • 1 National Key Laboratory of Green Pesticide, Integrative Microbiology Research Center, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou, China.
  • 2 National Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals of Guizhou University, Guiyang, China.
  • 3 Instrumental Analysis and Research Center, South China Agricultural University, Guangzhou, China.
  • 4 Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, China.
  • 5 Department of Biomedical Sciences, City University of Hong Kong, Kowloon Tong, Hong Kong.
  • 6 Shenzhen Research Institute, City University of Hong Kong, Shenzhen, China.
PMID: 37442803 DOI: 10.1002/ps.7664
Abstract

Background: Bacterial wilt induced by Ralstonia solanacearum is regarded as one of the most devastating diseases. However, excessive and repeated use of the same bactericides has resulted in development of Bacterial resistance. Targeting Bacterial virulence factors, such as type III secretion system (T3SS), without inhibiting Bacterial growth is a possible assay to discover new antimicrobial agents.

Results: In this work, identifying new T3SS inhibitors, a series of mandelic acid derivatives with 2-mercapto-1,3,4-thiazole moiety was synthesized. One of them, F-24, inhibited the transcription of hrpY gene significantly. The presence of this compound obviously attenuated hypersensitive response (HR) without inhibiting Bacterial growth of R. solanacearum. The transcription levels of those typical T3SS genes were reduced to various degrees. The test of the ability of F-24 in protecting Plants demonstrated that F-24 protected tomato Plants against Bacterial wilt without restricting the multiplication of R. solanacearum. The mechanism of this T3SS inhibition is through the PhcR-PhcA-PrhG-HrpB pathway.

Conculsion: The screened F-24 could inhibit R. solanacearum T3SS and showed better inhibitory activity than previously reported inhibitors without affecting the growth of the strain, and F-24 is a compound with good potential in the control of R. solanacearum. © 2023 Society of Chemical Industry.

Keywords

Ralstonia solanacearum; bacterial wilt; inhibitor; mandelic acid derivatives; mechanism; type III secretion system (T3SS).

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