2. Academic Validation
  3. Senkyunolide I ameliorates thoracic aortic aneurysm and dissection in mice via inhibiting the oxidative stress and apoptosis of endothelial cells

Senkyunolide I ameliorates thoracic aortic aneurysm and dissection in mice via inhibiting the oxidative stress and apoptosis of endothelial cells

  • Biochim Biophys Acta Mol Basis Dis. 2023 Oct;1869(7):166819. doi: 10.1016/j.bbadis.2023.166819.
Kaiwen Zhao 1,  Hongqiao Zhu 1,  Xiaomin He 1,  Pengcheng Du 1,  Taiping Liang 1,  Yudong Sun 2,  Zaiping Jing 3,  Jian Zhou 4
Affiliations

Affiliations

  • 1 Department of Vascular Surgery, the First Affiliated Hospital of the Navy Medical University, Shanghai, China.
  • 2 Depaertment of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, China.
  • 3 Department of Vascular Surgery, the First Affiliated Hospital of the Navy Medical University, Shanghai, China. Electronic address: [email protected].
  • 4 Department of Vascular Surgery, the First Affiliated Hospital of the Navy Medical University, Shanghai, China; Shanghai Key Laboratory of Vascular Lesions Regulation and Remodeling, Shanghai, China. Electronic address: [email protected].
PMID: 37499930 DOI: 10.1016/j.bbadis.2023.166819
Abstract

Background: Thoracic aortic aneurysm and aortic dissection (TAAD) is one of the most fatal cardiovascular diseases. Senkyunolide I (SEI) is a component of traditional Chinese medicine with remarkable anti-inflammatory properties and exhibits remarkable protective effects, but its impact on TAAD remains unclear. Our study aimed to explore the role of SEI in a murine model of TAAD and further explore the immunopharmacological mechanism.

Methods and materials: The in vivo model were assessed using echocardiography, gross anatomy, and tissue staining. Western blot and immunofluorescence were performed to evaluate the effects of SEI in vivo and in vitro. A SEI solution injection containing 1 % dimethyl sulfoxide (DMSO) was administered intraperitoneally to the TAAD model group, while a normal saline injection comprising 1 % DMSO was administered to the sham group.

Results: SEI prevented TAAD formation induced by BAPN/Ang II and reduced the TAAD incidence in mice. SEI treatment significantly inhibited the degradation of collagen and elastin fibers in the extracellular matrix. Furthermore, it reduced the expression of inflammatory factors in the aortic intima. Western blot analysis revealed that SEI-treated mice showed a significant decrease in apoptosis-related protein levels in the aorta compared with the TAAD group. PI3K, Akt, and mTOR in the SEI treatment group were significantly lower than in the model group. SEI could also attenuate H2O2-induced Human umbilical vein endothelial cells (HUVECs) damage and reverse the decline in migrant cells. The Apoptosis of HUVECs was considerably reduced by the SEI treatment.

Conclusions: Conclusively, SEI may alleviate the progression of TAAD by suppressing the PI3K/Akt/NF-κB signaling pathway. The SEI's ability to inhibit inflammation and oxidative stress opens the way to restore the function of endothelial cells and vascular homeostasis, and thus to provide novel and promising options for the treatment of TAAD patients.

Keywords

Aortic aneurysm; Aortic dissection; Apoptosis; Oxidative stress; Senkyunolide I.

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