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  3. Pyrrolidinedithiocarbamate ammonium

Pyrrolidinedithiocarbamate ammonium (Synonyms: Ammonium pyrrolidinedithiocarbamate; APDC; 1-Pyrrolidinedithiocarboxylic acid ammonium salt; PDTC)

Cat. No.: HY-18738 Purity: 99.86%
Handling Instructions

Pyrrolidinedithiocarbamate ammonium (Ammonium pyrrolidinedithiocarbamate) is a selective NF-κB inhibitor.

For research use only. We do not sell to patients.

Pyrrolidinedithiocarbamate ammonium Chemical Structure

Pyrrolidinedithiocarbamate ammonium Chemical Structure

CAS No. : 5108-96-3

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10 mM * 1 mL in DMSO USD 66 In-stock
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Customer Review

Based on 21 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Oncoimmunology. 2018 Aug 23;7(11):e1461303.

    The expression of Oct4 in A549 and H460 cells treated with or without rhIL-17A and these molecular inhibitors (NF-kB inhibitor: PDAC, Notch1 inhibitor: LY411575) are assessed by western blotting.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Oncoimmunology. 2018 Aug 23;7(11):e1461303.

    The expression of Oct4 in A549 and H460 cells treated with or without rhIL-17A and these molecular inhibitors (NF-kB inhibitor: PDAC, Notch1 inhibitor: LY411575) are assessed by western blotting.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Oxid Med Cell Longev. 2017;2017:3869561.

    N-formyl-Met-Leu-Phe (fMLP; 10 nM) are used to stimulate the cells for 2 min, and the polymorphonuclear neutrophils (PMNs) without fMLP serve as the control. NPS-2143 (100 nM), Cinacalcet (100 nM), and PDTC (100 nM) are added and incubated. Protein expression results are representative of three experiments.

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    Description

    Pyrrolidinedithiocarbamate ammonium (Ammonium pyrrolidinedithiocarbamate) is a selective NF-κB inhibitor.

    IC50 & Target[1]

    NF-κB

     

    In Vitro

    Pretreatment of cells with Pyrrolidinedithiocarbamate ammonium (Ammonium pyrrolidinedithiocarbamate; 3-1000 μM) dose-dependently attenuate IL-8 production[1].
    Furthermore, pyrrolidinedithiocarbamate ammonium (100 μM) suppresses the accumulation of IL-8 mRNA[1].
    Pyrrolidinedithiocarbamate ammonium inhibits the activation of NF-κB, because it suppresses both NF-κB DNA binding and NF-κB-dependent transcriptional activity. NF-κB inhibition with pyrrolidinedithiocarbamate ammonium decrease IL-8 production by intestinal epithelial cells[1].

    In Vivo

    The DSS+pyrrolidinedithiocarbamate ammonium-treated groupII exhibits suppression of shortening of intestinal length and reduction of DAI score. Activated NF-κB level and IL-1β and TNF-α levels are significantly lower in DSS+pyrrolidinedithiocarbamate ammonium-treated groupII. These findings suggest that suppression of NF-κB activity by pyrrolidinedithiocarbamate ammonium can delay the healing of mucosal tissue defects (erosions or ulcers) arising from inflammation, but that it can strongly suppress the expression of inf-lammatory cytokines (IL-1β and TNF-α), resulting in significant alleviation of colitis. pyrrolidinedithiocarbamate ammonium is useful for the treatment of ulcerative colitis[2].

    Molecular Weight

    164.29

    Formula

    C₅H₁₂N₂S₂

    CAS No.

    5108-96-3

    SMILES

    [S-]C(N1CCCC1)=S.[NH4+]

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 42 mg/mL (255.65 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 6.0868 mL 30.4340 mL 60.8680 mL
    5 mM 1.2174 mL 6.0868 mL 12.1736 mL
    10 mM 0.6087 mL 3.0434 mL 6.0868 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.08 mg/mL (12.66 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.08 mg/mL (12.66 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Cell Assay
    [1]

    The human colon cancer cell line HT-29 is obtained and cells are grown in modified McCoy’s 5A medium supplemented with 10% fetal bovine serum. To study the effect of pyrrolidinedithiocarbamate ammonium on IL-8 production, HT-29 cells in 96-well plates are induced with 20 ng/mL of IL-1β for 18 h. Various concentrations (3-1000 μM) of pyrrolidinedithiocarbamate or its vehicle (culture medium) are added to the cells 30 min prior to IL-1β stimulation. The concentration of IL-8 in the supernatant is determined using solid-phase enzyme-linked immunosorbent assay[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration

    Animal Administration: [2]Pyrrolidinedithiocarbamate is administered intraperitoneally to mice at dose levels of 100 and 50 mg/kg. Mice are divided into a DSS-untreated group (normal group), DSS-treated control group, DSS+pyrrolidinedithiocarbamate-treated groupI (low-dose group), and DSS+pyrrolidinedithiocarbamate-treated groupII (high-dose group). In each group, the disease activity index score (DAI score), intestinal length, histological score, and the levels of activated NF-κB and inflammatory cytokines (IL-1β and TNF-α) in tissue are measured[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Purity: 99.86%

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    Keywords:

    PyrrolidinedithiocarbamateAmmonium pyrrolidinedithiocarbamateAPDC1-Pyrrolidinedithiocarboxylic acidPDTCNF-κBNuclear factor-κBNuclear factor-kappaBulcerativecolitisIL-1βTNF-αinflammationInhibitorinhibitorinhibit

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    Product name:
    Pyrrolidinedithiocarbamate ammonium
    Cat. No.:
    HY-18738
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