1. NF-κB
  2. NF-κB

Pyrrolidinedithiocarbamate ammonium (Synonyms: Ammonium pyrrolidinedithiocarbamate; APDC; 1-Pyrrolidinedithiocarboxylic acid ammonium salt)

Cat. No.: HY-18738 Purity: 99.61%
Handling Instructions

Pyrrolidinedithiocarbamate ammonium is a selective NF-kB inhibitor.

For research use only. We do not sell to patients.

Pyrrolidinedithiocarbamate ammonium Chemical Structure

Pyrrolidinedithiocarbamate ammonium Chemical Structure

CAS No. : 5108-96-3

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 66 In-stock
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Estimated Time of Arrival: December 31
100 mg USD 60 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
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View All NF-κB Isoform Specific Products:

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References


Pyrrolidinedithiocarbamate ammonium is a selective NF-kB inhibitor.

IC50 & Target[1]



In Vitro

Pretreatment of cells with pyrrolidinedithiocarbamate (3-1000 μM) dose-dependently attenuate IL-8 production. Furthermore, pyrrolidinedithiocarbamate (100 μM) suppresses the accumulation of IL-8 mRNA. Pyrrolidinedithiocarbamate inhibits the activation of NF-kB, because pyrrolidinedithiocarbamate suppresses both NF-kB DNA binding and NF-kB-dependent transcriptional activity. NF-kB inhibition with pyrrolidinedithiocarbamate decrease IL-8 production by intestinal epithelial cells[1].

In Vivo

The DSS+pyrrolidinedithiocarbamate ammonium-treated groupII exhibits suppression of shortening of intestinal length and reduction of DAI score. Activated NF-κB level and IL-1β and TNF-α levels are significantly lower in DSS+pyrrolidinedithiocarbamate ammonium-treated groupII. These findings suggest that suppression of NF-κB activity by pyrrolidinedithiocarbamate ammonium can delay the healing of mucosal tissue defects (erosions or ulcers) arising from inflammation, but that it can strongly suppress the expression of inf-lammatory cytokines (IL-1β and TNF-α), resulting in significant alleviation of colitis. pyrrolidinedithiocarbamate ammonium is useful for the treatment of ulcerative colitis[2].

Clinical Trial
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 6.0868 mL 30.4340 mL 60.8680 mL
5 mM 1.2174 mL 6.0868 mL 12.1736 mL
10 mM 0.6087 mL 3.0434 mL 6.0868 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay

The human colon cancer cell line HT-29 is obtained and cells are grown in modified McCoy’s 5A medium supplemented with 10% fetal bovine serum. To study the effect of pyrrolidinedithiocarbamate ammonium on IL-8 production, HT-29 cells in 96-well plates are induced with 20 ng/mL of IL-1β for 18 h. Various concentrations (3-1000 μM) of pyrrolidinedithiocarbamate or its vehicle (culture medium) are added to the cells 30 min prior to IL-1β stimulation. The concentration of IL-8 in the supernatant is determined using solid-phase enzyme-linked immunosorbent assay[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration

Pyrrolidinedithiocarbamate is dissolved in distilled water.

Animal Administration: [2]Pyrrolidinedithiocarbamate is administered intraperitoneally to mice at dose levels of 100 and 50 mg/kg. Mice are divided into a DSS-untreated group (normal group), DSS-treated control group, DSS+pyrrolidinedithiocarbamate-treated groupI (low-dose group), and DSS+pyrrolidinedithiocarbamate-treated groupII (high-dose group). In each group, the disease activity index score (DAI score), intestinal length, histological score, and the levels of activated NF-κB and inflammatory cytokines (IL-1β and TNF-α) in tissue are measured[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight








Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: ≥ 42 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

Purity: 99.61%

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