Pyrrolidinedithiocarbamate ammonium

Pyrrolidinedithiocarbamate ammonium purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2023 Aug;44(8):1712-1724. [Abstract]

Customer Validation
Pyrrolidinedithiocarbamate ammonium (PDTC; 100 μM; 24 h) decreases viability of HepG2 and Huh7 cells on the basis of aspirin.

Pyrrolidinedithiocarbamate ammonium purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2022 Sep;12(9):3618-3638. [Abstract]

Customer Validation
SCT2 depletion-senescent LX2 cells are treated with rIL-1α protein and addressed in NF-κB inhibitor PDTC (100 μM), and the analysis of mRNAs of proinflammatory SASP IL1A, IL1B, IL6 and IL8 by qPCR.

Pyrrolidinedithiocarbamate ammonium purchased from MedChemExpress. Usage Cited in: Oncogene. 2022 Mar;41(13):1866-1881. [Abstract]

Customer Validation
The Cxcl10 mRNA expression in B16F0 cells treated with IFNα (1 000 U/ml) for 12 hours, in presence of PDTC (10 μM) or BAY 11-7082 (5 μM).

Pyrrolidinedithiocarbamate ammonium purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2022 Oct 21;S0141-8130(22)02401-1. [Abstract]

Customer Validation
TLR4 and downstream pathways are subjected to relevant western blotting. After the treatment with TJ-M2010, TRAF-stop, and PDTC (5 μM) for 1 h, DCs are stimulated with 10 μg/mL CARP2 for 0, 45, and 90 min.

Pyrrolidinedithiocarbamate ammonium purchased from MedChemExpress. Usage Cited in: JACC Basic Transl Sci. 2022 Aug 3;7(8):820-840. [Abstract]

Customer Validation
To pharmacologically inhibit NFκB, mice are given Pyrrolidinedithiocarbamate ammonium (PDTC; 100 mg/kg).

Pyrrolidinedithiocarbamate ammonium purchased from MedChemExpress. Usage Cited in: Clin Transl Med. 2022 Jun;12(6):e850. [Abstract]

Customer Validation
PEMs are treated with the NF-κB inhibitor PDTC (100 μM) for 1 h followed by BSA or UGRP1 (0.5 μg/mL) treatment and Pam3CSK4 stimulation for 6 h to measure Il6 mRNA by qRT‐PCR .

Pyrrolidinedithiocarbamate ammonium purchased from MedChemExpress. Usage Cited in: Clin Transl Med. 2022 Jun;12(6):e850. [Abstract]

Customer Validation
PEMs are treated with the NF-κB inhibitor PDTC (100 μM) for 1h followed by BSA or UGRP1 (0.5 μg/mL) treatment for 6 h, then stimulated by Pam3CSK4 for 0.5 h, p65 in the whole cell lysate and nucleus is analysed by immunoblot.

Pyrrolidinedithiocarbamate ammonium purchased from MedChemExpress. Usage Cited in: Redox Biol. 2021 Oct;46:102098. [Abstract]

Customer Validation
Several specific pharmacological inhibitors, including Pyrrolidine dithiocarbamate (PDTC, 100 μM), Wortmannin (Wort), Stattic, PD98059 and Bisindolylmaleimide I (Bis), are pre-administered to the cardiomyocytes in the HG + Pae group and then the expression of Opa1 is quantified.

Pyrrolidinedithiocarbamate ammonium purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Jan 19;11(7):3392-3416. [Abstract]

Customer Validation
Treatment with NF-κB (PDTC) inhibitor in both PC9 and H1975 cells treated with different concentrations of specific inhibitor for 72 h and Western blotting is performed.

Pyrrolidinedithiocarbamate ammonium purchased from MedChemExpress. Usage Cited in: EMBO Rep. 2021 Jul 5;22(7):e51678. [Abstract]

Customer Validation
qPCR analysis of Mmps including Mmp9, Mmp10, and Mmp12 from THP‐1 cells stimulated with AG (1 μg/mL) for 24 h in the absence or presence of PDTC at the concentration of 10 μM.

Pyrrolidinedithiocarbamate ammonium purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2019 Feb 8;10(2):113. [Abstract]

Customer Validation
After 1-h pretreatment with or without Pyrrolidinedithiocarbamate ammonium (PDTC, 100 μM) and 2-day co-culture with RANKL⁺ DSCs or ctrl DSCs, the MFI level of Foxp3 and TGF-β1 in d γδT (n = 8) were analyzed by FCM.

Pyrrolidinedithiocarbamate ammonium purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2019 Jan 24;15(3):688-700. [Abstract]

Customer Validation
T24 cells are treated with PDTC at various concentrations for 24-h, and cell viability and IC50 values are measured using MTS assays.

Pyrrolidinedithiocarbamate ammonium purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2019 Dec:120:109527. [Abstract]

Customer Validation
Treatment with aminoguanidine hydrochloride (AG, 100 mg/kg/d, dissolved in drinking water for 10 weeks), ALA , or PDTC (150 mg/kg/d, dissolved in drinking water for 10 weeks) significantly reduced the RAGE and 3‑NT levels in ZDF rats.

Pyrrolidinedithiocarbamate ammonium purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2019 Dec:120:109527. [Abstract]

Customer Validation
The protein expression of the NADPH oxidase subunits NOX2 and p22phox was significantly increased in the ZDF rats, while treatment with aminoguanidine hydrochloride (AG, 100 mg/kg/d, dissolved in drinking water for 10 weeks) or PDTC (150 mg/kg/d, dissolved in drinking water for 10 weeks) specifically reduced the NOX2 and p22phox levels in the ZDF rats.

Pyrrolidinedithiocarbamate ammonium purchased from MedChemExpress. Usage Cited in: Oncoimmunology. 2018 Aug 23;7(11):e1461303. [Abstract]

Customer Validation
The expression of Oct4 in A549 and H460 cells treated with or without rhIL-17A and these molecular inhibitors (NF-kB inhibitor: PDAC, Notch1 inhibitor: LY411575) are assessed by western blotting.

Pyrrolidinedithiocarbamate ammonium purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2017:2017:3869561. [Abstract]

Customer Validation
N-formyl-Met-Leu-Phe (fMLP; 10 nM) are used to stimulate the cells for 2 min, and the polymorphonuclear neutrophils (PMNs) without fMLP serve as the control. NPS-2143 (100 nM), AMG 073 (100 nM), and PDTC (100 nM) are added and incubated. Protein expression results are representative of three experiments.