2. Academic Validation
  3. Polydatin alleviates traumatic spinal cord injury by reducing microglial inflammation via regulation of iNOS and NLRP3 inflammasome pathway

Polydatin alleviates traumatic spinal cord injury by reducing microglial inflammation via regulation of iNOS and NLRP3 inflammasome pathway

  • Int Immunopharmacol. 2019 May;70:28-36. doi: 10.1016/j.intimp.2019.02.006.
Runxiao Lv 1,  Lili Du 2,  Xueyong Liu 1,  Fenghua Zhou 1,  Zhiqiang Zhang 3,  Lixin Zhang 4
Affiliations

Affiliations

  • 1 Department of Rehabilitation Medicine, Shengjing Hospital of China Medical University, Shenyang 110004, People's Republic of China.
  • 2 Department of Pathophysiology, College of Basic Medical Science, China Medical University, Shenyang 110122, People's Republic of China.
  • 3 Department of Rehabilitation Medicine, Shengjing Hospital of China Medical University, Shenyang 110004, People's Republic of China. Electronic address: [email protected].
  • 4 Department of Rehabilitation Medicine, Shengjing Hospital of China Medical University, Shenyang 110004, People's Republic of China. Electronic address: [email protected].
PMID: 30785088 DOI: 10.1016/j.intimp.2019.02.006
Abstract

Polydatin is a glucoside of resveratrol with lots of functional properties in the central nervous system, such as anti-edema, anti-oxidation and anti-inflammation. The purpose of this study was to evaluate the effects of polydatin on traumatic spinal cord injury (SCI) and explore the relative mechanisms. SCI models were established using the weight-drop method in rats, additionally, single polydatin administration (20, 40 mg/kg body weight) remarkably improved motor function of SCI rat, along with decreased nitric oxide (NO) generation and inflammatory factor (IL-1β, IL-6 and TNF-α) production in spinal cord tissues. Similar to the results of in vivo experiments, the inflammatory response was aggravated with the intervention of lipopolysaccharide (LPS) in BV2 microglia. However, polydatin treatment (1, 2 and 4 μM) inhibited iNOS expression, decreased NLRP3 inflammasome activation, which subsequently relieved microglial inflammation. Above all, our data indicated that polydatin possessed neuroprotective effects in SCI rats, possibly by suppressing iNOS expression and NLRP3 inflammasome activation in microglia.

Keywords

Microglia; NLRP3 inflammasome; NO; Polydatin; Spinal cord injury.

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