2. Academic Validation
  3. Protocol for detection of ferroptosis in cultured cells

Protocol for detection of ferroptosis in cultured cells

  • STAR Protoc. 2023 Aug 8;4(3):102457. doi: 10.1016/j.xpro.2023.102457.
Magdalena B Murray 1,  Logan B Leak 2,  Weaverly Colleen Lee 2,  Scott J Dixon 3
Affiliations

Affiliations

  • 1 Department of Biology, Stanford University, 327 Campus Dr, Stanford, CA 94305, USA. Electronic address: [email protected].
  • 2 Department of Biology, Stanford University, 327 Campus Dr, Stanford, CA 94305, USA.
  • 3 Department of Biology, Stanford University, 327 Campus Dr, Stanford, CA 94305, USA. Electronic address: [email protected].
PMID: 37556320 DOI: 10.1016/j.xpro.2023.102457
Abstract

Mammalian cells can die by Apoptosis or by one of several non-apoptotic mechanisms, such as Ferroptosis. Here, we present a protocol to distinguish Ferroptosis from other cell death mechanisms in cultured cells. We describe steps for seeding cells, administering mechanism-specific cell death inducers and inhibitors, and measuring cell death and viability. We then detail the use of molecular markers to verify mechanisms of cell death. This protocol can be used to identify and distinguish Ferroptosis in 2D and 3D cultures. For complete details on the use and execution of this protocol, please refer to Ko, et al. (2019),1 Magtanong, et al. (2022),2 and Armenta, et al. (2022).3.

Keywords

Cancer; Cell biology; Cell culture; Cell-based assays.

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