2. Academic Validation
  3. Synthesis of flurbiprofen thiadiazole urea derivatives and assessment of biological activities and molecular docking studies

Synthesis of flurbiprofen thiadiazole urea derivatives and assessment of biological activities and molecular docking studies

  • Chem Biol Drug Des. 2023 Aug 31. doi: 10.1111/cbdd.14336.
Belma Zengin Kurt 1,  Özlem Altundağ 2,  Merve Nur Tokgöz 2,  Dilek Öztürk Civelek 3,  Fulya Oz Tuncay 4,  Ummuhan Cakmak 4,  Yakup Kolcuoğlu 4,  Atilla Akdemir 5,  Fatih Sönmez 6
Affiliations

Affiliations

  • 1 Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Bezmialem Vakif University, Istanbul, Türkiye.
  • 2 Faculty of Pharmacy, Bezmialem Vakif University, Istanbul, Türkiye.
  • 3 Faculty of Pharmacy, Department of Pharmacology, Bezmialem Vakif University, Istanbul, Türkiye.
  • 4 Faculty of Science, Department of Chemistry, Karadeniz Technical University, Trabzon, Türkiye.
  • 5 Faculty of Pharmacy, Department of Pharmacology, Istinye University, Istanbul, Türkiye.
  • 6 Pamukova Vocational School, Sakarya University of Applied Sciences, Sakarya, Türkiye.
PMID: 37653693 DOI: 10.1111/cbdd.14336
Abstract

Totally 15 novel flurbiprofen urea derivatives were synthesized bearing the thiadiazole ring. Their inhibition effects on Tyrosinase were determined. 3c was found to be the strongest inhibitor with the IC50 value of 68.0 μM against Tyrosinase. The Enzyme inhibition types of the synthesized compounds were determined by examining the kinetic parameters. The inhibition type of 3c was determined as uncompetitive and the Ki value was calculated as 36.3 μM. Moreover, their cytotoxic effects on hepatocellular carcinoma (HepG2), colorectal carcinoma (HT-29), and melanoma (B16F10) cell lines were evaluated. According to the cytotoxicity results, 3l (IC50 = 14.11 μM) showed the highest cytotoxicity on the HT-29 cells, while 3o (IC50 = 4.22 μM) exhibited the strongest cytotoxic effect on HepG2 cell lines. Also, 3j (IC50 = 7.55 μM strongly affected B16F10. The effects of synthesized compounds on the healthy cell line were evaluated on the CCD-986Sk cell line. Molecular modelling studies have indicated the potential binding interactions of the uncompetitive inhibitor 3c with the enzyme-substrate complex.

Keywords

cytotoxcity; flurbiprofen; molecular docking; thiadiazole ring; tyrosinase inhibition; urea.

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