2. Academic Validation
  3. Nuclear and cytoplasmic USP30-AS1 coordinately regulate breast cancer progression through HnRNPF/p21 and EZH2/c-Myc/p21 axes

Nuclear and cytoplasmic USP30-AS1 coordinately regulate breast cancer progression through HnRNPF/p21 and EZH2/c-Myc/p21 axes

  • Genes Dis. 2025 May 10;13(2):101684. doi: 10.1016/j.gendis.2025.101684.
Yapei Jiang 1,  Weijie Liao 1 2,  Qilei Xin 1,  Ruonan Wang 1,  Guanglan Lin 1,  Jia Li 1,  Zijian Yang 3,  Shiyue Yang 1,  Haowei Zhang 1,  Xiaolin Li 1,  Qian Peng 1,  Yaou Zhang 1,  Weidong Xie 1,  Naihan Xu 4
Affiliations

Affiliations

  • 1 State Key Laboratory of Chemical Oncogenomics, Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong 518055, China.
  • 2 Department of Hematology and Oncology, International Cancer Center, Shenzhen University General Hospital, Shenzhen University, Shenzhen, Guangdong 518000, China.
  • 3 Department of Breast and Thyroid Surgery, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518034, China.
  • 4 School of Food and Drug, Shenzhen Polytechnic University, Shenzhen, Guangdong 518055, China.
PMID: 41492473 DOI: 10.1016/j.gendis.2025.101684
Abstract

Emerging evidence suggests that aberrant expression of long non-coding RNAs (lncRNAs) is strongly associated with the occurrence and progression of breast Cancer. Herein, we identified ubiquitin specific peptidase 30 antisense RNA 1 (USP30-AS1) as a markedly upregulated lncRNA in breast Cancer tissues, and the transcription factor SPI1 functions upstream to regulate the expression of USP30-AS1. Gene set enrichment analysis suggests that USP30-AS1 may regulate cell proliferation. Knockdown of USP30-AS1 suppresses breast Cancer cell proliferation and tumor growth by up-regulating CDKN1A/p21. Mechanistically, USP30-AS1 exhibits dual localization within breast Cancer cells. In the cytoplasm, it interacts with HnRNPF, disrupting its binding to the p21 3'UTR, which destabilizes p21 mRNA and ultimately reduces p21 expression. In the nucleus, USP30-AS1 suppresses p21 transcription by enhancing the activity of c-Myc, a known transcriptional repressor of p21. USP30-AS1 binds to enhancer of zeste homolog 2 (EZH2), a Histone Methyltransferase, and prevents EZH2 from binding to the c-Myc promoter. This promotes epigenetic up-regulation of c-Myc by reducing H3K27 trimethylation. Together, these findings demonstrate the critical role of USP30-AS1 in breast Cancer progression through HnRNPF/p21 and EZH2/c-Myc/p21 axes, highlighting its potential as a therapeutic target for breast Cancer treatment.

Keywords

Breast cancer; CDKN1A/p21; HnRNPF; USP30-AS1; c-Myc.

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