AKT1 Protein, Human (sf9, His)

AKT1 Protein, part of the AKT kinase family, crucially regulates metabolism, proliferation, survival, growth, and angiogenesis. Phosphorylating over 100 substrates, it orchestrates insulin-induced SLC2A4/GLUT4 translocation, impacting glucose uptake, and governs glycogen storage by inhibiting GSK3A/B. AKT1 mediates insulin-stimulated protein synthesis via mTORC1, regulates FOXO factors, influences NF-kappa-B-dependent gene transcription, and enhances CREB1 activity. It modulates lipid metabolism, inhibiting lipolysis, and is involved in cell migration, adhesion, placental development, and responses to growth factors like PDGF, EGF, insulin, and IGF-I. Its role in SPATA13-mediated regulation adds complexity, highlighting AKT1's central role in cellular homeostasis and responsiveness to cues. AKT1 Protein, Human (sf9, His) is the recombinant human-derived AKT1 protein, expressed by Sf9 insect cells, with C-His labeled tag. The total length of AKT1 Protein, Human (sf9, His) is 480 a.a., with molecular weight of ~57 kDa.

AKT1 Protein, a member of the AKT kinase family alongside AKT2 and AKT3, plays a pivotal role in regulating diverse cellular processes such as metabolism, proliferation, cell survival, growth, and angiogenesis. Functioning through serine and/or threonine phosphorylation of numerous downstream substrates, AKT1 is implicated in the modulation of over 100 reported substrate candidates, demonstrating its multifaceted nature. Notably, it orchestrates the insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter, impacting glucose uptake. Furthermore, AKT1 governs glucose storage as glycogen by phosphorylating GSK3A and GSK3B, inhibiting their kinase activity. Its involvement in various pathways includes mediating insulin-stimulated protein synthesis through the mTORC1 signaling pathway and regulating the FOXO factors, impacting cellular localization. AKT1 also influences NF-kappa-B-dependent gene transcription and positively regulates CREB1 activity, contributing to the transcription of pro-survival genes. Additionally, AKT1 exhibits a role in lipid metabolism by phosphorylating ATP citrate lyase (ACLY) and activates the 3B isoform of cyclic nucleotide phosphodiesterase (PDE3B), resulting in reduced cyclic AMP levels and inhibition of lipolysis. Beyond these functions, AKT1 is implicated in cell migration, adhesion assembly and disassembly, placental development, and diverse cellular responses to growth factors, including platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin, and insulin-like growth factor I (IGF-I). The recent discovery of its role in the SPATA13-mediated regulation of cell migration and adhesion adds to the complexity of its functions, highlighting AKT1's central role in cellular homeostasis and responsiveness to environmental cues.

No Kinase Activity.

Human

Sf9 insect cells

C-His

P31749 (M1-A480)

207  [NCBI]

Approximately 57 kDa

Greater than 87% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 3 mM DTT, 0.5 mM PMSF, 5% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.