Aminopeptidase N/APN Protein, Human (I603M, HEK293, His)

Aminopeptidase N/APN protein is a disulfide-bonded homotrimer functioning as a broad specificity aminopeptidase. It plays a crucial role in peptide digestion, peptide processing, antigen presentation, angiogenesis, and cholesterol crystallization. APN protein also interacts with the amino acid transporter SLC6A19 and acts as a receptor for human coronavirus 229E/HCoV-229E during infection. Aminopeptidase N/APN Protein, Human (I603M, HEK293, His) is the recombinant human-derived Aminopeptidase N/APN protein, expressed by HEK293 , with C-His labeled tag and I603M mutation. The total length of Aminopeptidase N/APN Protein, Human (I603M, HEK293, His) is 899 a.a., with molecular weight of 135-140 kDa.

Aminopeptidase N/APN protein is a broad specificity aminopeptidase that functions as a homotrimer connected by disulfide bonds. It plays a crucial role in the final digestion of peptides derived from protein hydrolysis by gastric and pancreatic proteases. Moreover, it is involved in the processing of various peptides, including peptide hormones like angiotensin III and IV, neuropeptides, and chemokines. Additionally, it may participate in the cleavage of peptides bound to major histocompatibility complex class II molecules in antigen presenting cells. This protein may also contribute to angiogenesis and promote cholesterol crystallization. Furthermore, it acts as a binding partner of the amino acid transporter SLC6A19, potentially regulating its activity and impacting amino acid transport. Notably, Aminopeptidase N/APN protein acts as a receptor for human coronavirus 229E/HCoV-229E, particularly serving as a receptor for the spike glycoprotein of HCoV-229E during infection.

Human

HEK293

C-His

P15144/NP_001141.2 (K69-K967, I603M)

290  [NCBI]

KAWNRYRLPNTLKPDSYRVTLRPYLTPNDRGLYVFKGSSTVRFTCKEATDVIIIHSKKLNYTLSQGHRVVLRGVGGSQPPDIDKTELVEPTEYLVVHLKGSLVKDSQYEMDSEFEGELADDLAGFYRSEYMEGNVRKVVATTQMQAADARKSFPCFDEPAMKAEFNITLIHPKDLTALSNMLPKGPSTPLPEDPNWNVTEFHTTPKMSTYLLAFIVSEFDYVEKQASNGVLIRIWARPSAIAAGHGDYALNVTGPILNFFAGHYDTPYPLPKSDQIGLPDFNAGAMENWGLVTYRENSLLFDPLSSSSSNKERVVTVIAHELAHQWFGNLVTIEWWNDLWLNEGFASYVEYLGADYAEPTWNLKDLMVLNDVYRVMAVDALASSHPLSTPASEINTPAQISELFDAISYSKGASVLRMLSSFLSEDVFKQGLASYLHTFAYQNTIYLNLWDHLQEAVNNRSIQLPTTVRDIMNRWTLQMGFPVITVDTSTGTLSQEHFLLDPDSNVTRPSEFNYVWIVPITSIRDGRQQQDYWLMDVRAQNDLFSTSGNEWVLLNLNVTGYYRVNYDEENWRKIQTQLQRDHSAIPVINRAQIINDAFNLASAHKVPVTLALNNTLFLIEERQYMPWEAALSSLSYFKLMFDRSEVYGPMKNYLKKQVTPLFIHFRNNTNNWREIPENLMDQYSEVNAISTACSNGVPECEEMVSGLFKQWMENPNNNPIHPNLRSTVYCNAIAQGGEEEWDFAWEQFRNATLVNEADKLRAALACSKELWILNRYLSYTLNPDLIRKQDATSTIISITNNVIGQGLVWDFVQSNWKKLFNDYGGGSFSFSNLIQAVTRRFSTEYELQQLEQFKKDNEETGFGSGTRALEQALEKTKANIKWVKENKEVVLQWFTENSK

135-140 kDa

Greater than 95% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.