Aminopeptidase N/APN Protein, Human (HEK293, His)

Aminopeptidase N/APN protein is a disulfide-bonded homotrimer functioning as a broad specificity aminopeptidase. It plays a crucial role in peptide digestion, peptide processing, antigen presentation, angiogenesis, and cholesterol crystallization. APN protein also interacts with the amino acid transporter SLC6A19 and acts as a receptor for human coronavirus 229E/HCoV-229E during infection. Aminopeptidase N/APN Protein, Human (HEK293, His) is the recombinant human-derived Aminopeptidase N/APN protein, expressed by HEK293 , with C-His labeled tag.

Aminopeptidase N/APN protein is a broad specificity aminopeptidase that functions as a homotrimer connected by disulfide bonds. It plays a crucial role in the final digestion of peptides derived from protein hydrolysis by gastric and pancreatic proteases. Moreover, it is involved in the processing of various peptides, including peptide hormones like angiotensin III and IV, neuropeptides, and chemokines. Additionally, it may participate in the cleavage of peptides bound to major histocompatibility complex class II molecules in antigen presenting cells. This protein may also contribute to angiogenesis and promote cholesterol crystallization. Furthermore, it acts as a binding partner of the amino acid transporter SLC6A19, potentially regulating its activity and impacting amino acid transport. Notably, Aminopeptidase N/APN protein acts as a receptor for human coronavirus 229E/HCoV-229E, particularly serving as a receptor for the spike glycoprotein of HCoV-229E during infection.

Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is >2500 pmoL/min/µg, as measured under the described conditions.

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  Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is 1.3946×10⁴ pmol/min/µg, as measured under the described conditions.

Human

HEK293

C-His

P15144 (K69-K967)

290  [NCBI]

Partial

KAWNRYRLPNTLKPDSYRVTLRPYLTPNDRGLYVFKGSSTVRFTCKEATDVIIIHSKKLNYTLSQGHRVVLRGVGGSQPPDIDKTELVEPTEYLVVHLKGSLVKDSQYEMDSEFEGELADDLAGFYRSEYMEGNVRKVVATTQMQAADARKSFPCFDEPAMKAEFNITLIHPKDLTALSNMLPKGPSTPLPEDPNWNVTEFHTTPKMSTYLLAFIVSEFDYVEKQASNGVLIRIWARPSAIAAGHGDYALNVTGPILNFFAGHYDTPYPLPKSDQIGLPDFNAGAMENWGLVTYRENSLLFDPLSSSSSNKERVVTVIAHELAHQWFGNLVTIEWWNDLWLNEGFASYVEYLGADYAEPTWNLKDLMVLNDVYRVMAVDALASSHPLSTPASEINTPAQISELFDAISYSKGASVLRMLSSFLSEDVFKQGLASYLHTFAYQNTIYLNLWDHLQEAVNNRSIQLPTTVRDIMNRWTLQMGFPVITVDTSTGTLSQEHFLLDPDSNVTRPSEFNYVWIVPITSIRDGRQQQDYWLIDVRAQNDLFSTSGNEWVLLNLNVTGYYRVNYDEENWRKIQTQLQRDHSAIPVINRAQIINDAFNLASAHKVPVTLALNNTLFLIEERQYMPWEAALSSLSYFKLMFDRSEVYGPMKNYLKKQVTPLFIHFRNNTNNWREIPENLMDQYSEVNAISTACSNGVPECEEMVSGLFKQWMENPNNNPIHPNLRSTVYCNAIAQGGEEEWDFAWEQFRNATLVNEADKLRAALACSKELWILNRYLSYTLNPDLIRKQDATSTIISITNNVIGQGLVWDFVQSNWKKLFNDYGGGSFSFSNLIQAVTRRFSTEYELQQLEQFKKDNEETGFGSGTRALEQALEKTKANIKWVKENKEVVLQWFTENSK

130-140 kDa

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4-7.3.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.