Apolipoprotein E/APOE3 Protein, Human (HEK293, C-His)

Apolipoprotein E (APOE) plays a pivotal role in lipoprotein-mediated lipid transport, acting as a core component in plasma lipoproteins' production, conversion, and clearance. As an amphipathic molecule, APOE associates with various lipoprotein particles, including chylomicrons, chylomicron remnants, VLDL, and IDL, favoring HDL. It engages with cellular receptors, such as LDLR, LRP1, LRP2, LRP8, and VLDLR, facilitating cellular uptake of APOE-containing lipoproteins. APOE's functions include mediating lipoprotein clearance, participating in biosynthesis, and uptake of VLDLs by peripheral tissues for triglyceride delivery, contributing to lipid homeostasis, reverse cholesterol transport, and playing diverse roles in the central nervous system, immune responses, and transcriptional regulation. Apolipoprotein E/APOE Protein, Human (HEK293, C-His) is the recombinant human-derived Apolipoprotein E/APOE protein, expressed by HEK293, with C-6*His labeled tag. The total length of Apolipoprotein E/APOE Protein, Human (HEK293, C-His) is 298 a.a., with molecular weight of ~36.36 kDa.

Apolipoprotein E (APOE) is a crucial player in lipoprotein-mediated lipid transport, serving as a core component of plasma lipoproteins involved in their production, conversion, and clearance. Functioning as an amphipathic molecule, APOE associates with various lipoprotein particles, including chylomicrons, chylomicron remnants, very low-density lipoproteins (VLDL), and intermediate density lipoproteins (IDL), with a preference for high-density lipoproteins (HDL). It engages with a range of cellular receptors, such as the LDL receptor (LDLR), LDL receptor-related proteins (LRP1, LRP2, and LRP8), and the very low-density lipoprotein receptor (VLDLR), facilitating cellular uptake of APOE-containing lipoprotein particles. Additionally, APOE exhibits heparin-binding activity, interacting with heparan-sulfate proteoglycans on cell surfaces, supporting the capture and receptor-mediated uptake of APOE-containing lipoproteins. APOE's main function involves mediating lipoprotein clearance through hepatocyte uptake and participating in the biosynthesis and uptake of VLDLs by peripheral tissues for triglyceride delivery and energy storage. It crucially contributes to lipid homeostasis, participating in reverse cholesterol transport and playing roles in the central nervous system, immune responses, and transcriptional regulation, notably in interactions with HCV during microbial infection.

Human

HEK293

C-6*His

P02649 (K19-N316)

348  [NCBI]

KVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH

Approximately 36.36 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.