Apolipoprotein E/APOE3 Protein, Human (HEK293, His)

Apolipoprotein E/ApoE3 Protein, Human (HEK293, His) expresses in HEK293 with a His tag at the N-terminus. Apolipoprotein E/ApoE Protein, Human (HEK293, His) promotes cholesterol release in an isoform-dependent manner^[1][2].

ApoE isoform specifically inhibits lipid-particle-mediated cholesterol release from neurons. Although apoE and a lipid particle are lipid acceptors, when apoE and a lipid particle form a complex, apoE on the particle surface inhibits the lipid particle-mediated cholesterol release from cells in an apoE-concentration-dependent manner^[2].

Measured in a cell proliferation assay using SH-SY5Y cells.The ED₅₀ for this effect is ≤15.14 ng/mL, corresponding to a specific activity is ≥6.61×10⁴ units/mg.

• 
  Measured in a cell proliferation assay using SH-SY5Y cells.The ED₅₀ for this effect is 15.14ng/ml.corresponding to a specific activity is 6.61×10⁴ units/mg.

Human

HEK293

C-6*His

P02649 (K19-H317)

348  [NCBI]

KVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH

36-39 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 5% Trehalose,5% Mannitol, 0.02% Tween80, pH 8.0 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Gong JS, et al. Apolipoprotein E (ApoE) isoform-dependent lipid release from astrocytes prepared from humanApoE3 and ApoE4 knock-in mice. J Biol Chem. 2002 Aug 16;277(33):29919-26.  [Content Brief]

  [2]. Gong JS, et al. Novel action of apolipoprotein E (ApoE): ApoE isoform specifically inhibits lipid-particle-mediated cholesterol release from neurons. Mol Neurodegener. 2007 May 15;2:9.  [Content Brief]