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Apolipoprotein E/APOE3 Protein, Human (HEK293, His)

Cat. No.: HY-P7531
COA Handling Instructions

Apolipoprotein E/ApoE3 Protein, Human (HEK293, His) expresses in HEK293 with a His tag at the N-terminus. Apolipoprotein E/ApoE Protein, Human (HEK293, His) promotes cholesterol release in an isoform-dependent manner.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

Apolipoprotein E/ApoE3 Protein, Human (HEK293, His) expresses in HEK293 with a His tag at the N-terminus. Apolipoprotein E/ApoE Protein, Human (HEK293, His) promotes cholesterol release in an isoform-dependent manner[1][2].

Background

ApoE isoform specifically inhibits lipid-particle-mediated cholesterol release from neurons. Although apoE and a lipid particle are lipid acceptors, when apoE and a lipid particle form a complex, apoE on the particle surface inhibits the lipid particle-mediated cholesterol release from cells in an apoE-concentration-dependent manner[2].

Biological Activity

Measured in a cell proliferation assay using SH-SY5Y cells.The ED50 for this effect is ≤15.14 ng/mL, corresponding to a specific activity is ≥6.61×104 units/mg.

  • Measured in a cell proliferation assay using SH-SY5Y cells.The ED50 for this effect is 15.14ng/ml.corresponding to a specific activity is 6.61×104 units/mg.
Species

Human

Source

HEK293

Tag

C-6*His

Accession

P02649 (K19-H317)

Gene ID

348  [NCBI]

Synonyms
rHuApolipoprotein E, His; ApoE; Apolipoprotein E; APOE3
AA Sequence

KVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH

Molecular Weight

36-39 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 5% Trehalose,5% Mannitol, 0.02% Tween80, pH 8.0 or 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

Apolipoprotein E/APOE3 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Apolipoprotein E/APOE3 Protein, Human (HEK293, His)
Cat. No.:
HY-P7531
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