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ASAM/CLMP Protein, Rat (HEK293, His)

Cat. No.: HY-P72837
COA Handling Instructions

ASAM/CLMP proteins have emerged as potential key players in cell-cell adhesion, implicating their role in maintaining tissue integrity. Indications of involvement in adipocyte differentiation suggest potential effects on adipose tissue development and obesity regulation. ASAM/CLMP Protein, Rat (HEK293, His) is the recombinant rat-derived ASAM/CLMP protein, expressed by HEK293 , with C-His labeled tag. The total length of ASAM/CLMP Protein, Rat (HEK293, His) is 232 a.a., with molecular weight of ~28-35 kDa.

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Description

ASAM/CLMP proteins have emerged as potential key players in cell-cell adhesion, implicating their role in maintaining tissue integrity. Indications of involvement in adipocyte differentiation suggest potential effects on adipose tissue development and obesity regulation. ASAM/CLMP Protein, Rat (HEK293, His) is the recombinant rat-derived ASAM/CLMP protein, expressed by HEK293 , with C-His labeled tag. The total length of ASAM/CLMP Protein, Rat (HEK293, His) is 232 a.a., with molecular weight of ~28-35 kDa.

Background

ASAM/CLMP Protein emerges as a potential key player in various cellular processes, with indications of involvement in cell-cell adhesion, suggesting its role in the maintenance of tissue integrity. Furthermore, ASAM/CLMP may play a significant role in adipocyte differentiation, hinting at its potential impact on adipose tissue development and, consequently, the regulation of obesity. Additionally, its requirement for normal small intestine development underscores its importance in the intricate processes governing gastrointestinal tissue formation. Exploring the specific mechanisms by which ASAM/CLMP contributes to cell adhesion, adipocyte differentiation, and intestinal development could provide valuable insights into its multifaceted functions and its potential implications in physiological and pathological contexts.

Species

Rat

Source

HEK293

Tag

C-6*His

Accession

Q8K1G0 (T18-M232)

Gene ID

286939  [NCBI]

Molecular Construction
N-term
ASAM (M1-M232)
Accession # Q8K1G0
His
C-term
Synonyms
CXADR-like membrane protein; Adipocyte-specific protein 5; Acam; Asp5; ASAM; CLMP
AA Sequence

THTEIKRVAEEKVTLPCHHQLGLPEKDTLDIEWLLTDNEGNQKVVITYSSRHVYNNLTEEQKGRVAFASNFLAGDASLQIEPLKPSDEGRYTCKVKNSGRYVWSHVILKVLVRPSKPKCELEGEPTEGSDLTLQCESASGTKPIVYYWQRIREKEGEDEHLPPKSRIDYNNPGRVLLQNLTMASSGLYQCTAGNEAGKESCVVRVTVQYVQSIGM

Molecular Weight

Approximately 28-35 kDa due to the glycosylation

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

ASAM/CLMP Protein, Rat (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ASAM/CLMP Protein, Rat (HEK293, His)
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HY-P72837
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