BACE1 Protein, Mouse (HEK293, His)

BACE1 protein is an important member of the peptidase A1 family and is involved in the pathogenesis of Alzheimer's disease. They produce mature proteases through proteolytic processing of preprotein, which catalyze the initial steps in the formation of amyloid beta peptide from the amyloid precursor protein. BACE1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived BACE1 protein, expressed by HEK293 , with C-His labeled tag. The total length of BACE1 Protein, Mouse (HEK293, His) is 436 a.a., with molecular weight of 60-76 kDa.

BACE1, a member of the peptidase A1 family of aspartic proteases, plays a pivotal role in the pathogenesis of Alzheimer's disease. This gene gives rise to multiple transcript variants, one of which encodes a preproprotein undergoing proteolytic processing to generate the mature protease. Functioning as a transmembrane protease, BACE1 catalyzes the initial step in the formation of amyloid beta peptides from amyloid precursor protein. These peptides constitute the primary components of amyloid beta plaques, prominent in the brains of individuals with Alzheimer's disease. Homozygous knockout mice for BACE1 display a spectrum of nervous system defects, growth retardation, metabolic abnormalities, and elevated neonatal lethality. The broad expression of BACE1 across various tissues, including the cerebellum and ovary, underscores its significance beyond the central nervous system.

Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2. Read at excitation and emission wavelengths of 320 nm and 405 nm. The specific activity is 1035.061 pmoL/min/µg, as measured under the described conditions.

Mouse

HEK293

C-His

NP_035922.4 (T22-T457)

23821  [NCBI]

THLGIRLPLRSGLAGPPLGLRLPRETDEESEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHIPNIFSLQLCGAGFPLNQTEALASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAVSQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTADMEDCGYNIPQTDESTLMT

60-76 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.