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COMMD8 Protein, Human (N-His)

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COMMD8 Protein, Human (N-His) expresses in E.coli with a His tag at the N-terminus. COMMD8 is a protein that binds to the C-terminal amino acid sequence (residues 306–352) of human CXCR4.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
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Biological Activity

Description

COMMD8 Protein, Human (N-His) expresses in E.coli with a His tag at the N-terminus. COMMD8 is a protein that binds to the C-terminal amino acid sequence (residues 306–352) of human CXCR4[1].

Background

COMMD8 is a scaffold protein in the commander complex, essential for endosomal recycling of transmembrane cargos. The commander complex consists of the CCC subcomplex and the retriever subcomplex. It may modulate the activity of cullin-RING E3 ubiquitin ligase (CRL) complexes and down-regulate NF-kappa-B activation.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-6*His
  • Accession

    AAH19826 (M1-K183)

  • Gene ID
    54951  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • COMMD8 (M1-K183)
      Accession # AAH19826
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    COMMD8; COMM Domain-Containing Protein 8; COMM Domain Containing 8; FLJ20502

  • AA Sequence

    MEPEEGTPLWRLQKLPAELGPQLLHKIIDGICGRAYPVYQDYHTVWESEEWMHVLEDIAKFFKAIVGKNLPDEEIFQQLNQLNSLHQETIMKCVKSRKDEIKQALSREIVAISSAQLQDFDWQVKLLLSSDKIAALRMPLLSLHLDVKENGEVKPYSIEMSREELQNLIQSLEAANKVVLQLK

  • Predicted Molecular Mass

    22 kDa

  • Molecular Weight

    Approximately 23 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. kiko Nakai, et al. The COMMD3/8 complex determines GRK6 specificity for chemoattractant receptors. J Exp Med. 2019 Jul 1;216(7):1630-1647. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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