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FLT3 Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

FLT3, a receptor tyrosine kinase, has diverse functions including kinase activation, PI3K binding, and ligase binding. It plays a vital role in hematopoiesis, immune function, and tissue development. FLT3 is expressed in various tissues like the brain, immune system, and reproductive system. It is involved in diseases like leukemia and cancer. The wide functions and tissue-specific expression emphasize FLT3's importance in health and disease. FLT3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FLT3 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for FLT3 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for FLT3 Protein, Mouse (HEK293, His)
  • FLT3 Protein, Mouse (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

FLT3, a receptor tyrosine kinase, has diverse functions including kinase activation, PI3K binding, and ligase binding. It plays a vital role in hematopoiesis, immune function, and tissue development. FLT3 is expressed in various tissues like the brain, immune system, and reproductive system. It is involved in diseases like leukemia and cancer. The wide functions and tissue-specific expression emphasize FLT3's importance in health and disease. FLT3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FLT3 protein, expressed by HEK293 , with C-His labeled tag.

Background

FLT3, a receptor tyrosine kinase, possesses diverse functional capabilities, including phosphatidylinositol 3-kinase binding, protein tyrosine kinase activity, and ubiquitin protein ligase binding. Its involvement in critical processes such as common myeloid progenitor cell proliferation, hemopoiesis, and lymphocyte proliferation highlights its essential role in hematopoietic development and immune system function. Situated on the external side of the plasma membrane, FLT3 exhibits biased expression in various tissues, including the brain, dorsal aorta, immune and reproductive systems, and spinal cord. This protein serves as a crucial player in diseases like acute myeloid leukemia and juvenile myelomonocytic leukemia, and its orthologs are implicated in conditions such as acute lymphoblastic leukemia, colorectal adenocarcinoma, hepatocellular carcinoma, and prostate cancer. The multifaceted functions and tissue-specific expression underscore the significance of FLT3 in cellular homeostasis and disease progression.

Verified Bioactivity

1.This product does not contain protein kinase domain.
2.Measured by its binding ability in a functional ELISA. Immobilized human Flt-3 Ligand, at 1 μg/mL (100 μL/well) can bind Biotinylated Mouse FLT3 protein. The ED50 for this effect is 5.987 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized human Flt-3 Ligand, at 1 μg/mL (100 μL/well) can bind Biotinylated Mouse FLT3 protein. The ED50 for this effect is 5.987 ng/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-His
  • Accession

    Q3UEW6/NP_034359.2 (N28-S544)

  • Gene ID
    14255  [NCBI]
  • Molecular Construction
    • N-term
    • FLT3 (N28-S544)
      Accession # Q3UEW6/NP_034359.2
    • His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    FLT3; CD135 Antigen; Fms Related Receptor Tyrosine Kinase 3; FLK-2; CD135; Growth Factor Receptor Tyrosine Kinase Type III; STK1; Receptor Protein-Tyrosine Kinase; FLK2; Fms-Related Tyrosine Kinase 3; Fms-Like Tyrosine Kinase 3; Fetal Liver Kinase 2; Rece

  • AA Sequence

    NQDLPVIKCVLISHENNGSSAGKPSSYRMVRGSPEDLQCAPRRQSEGTVYEAATVEVAESGSITLQVQLATPGDLSCLWVFKHSSLGCQPHFDLQNRGIVSMAILNVTETQAGEYLLHIQSEAANYTVLFTVNVRDTQLYVLRRPYFRKMENQDALLCISEGVPEPTVEWVLCSSHRESCKEEGPAVVRKEEKVLHELFGTDIRCCARNALGRECTKLFTIDLNQAPQSTLPQLFLKVGEPLWIRCKAIHVNHGFGLTWELEDKALEEGSYFEMSTYSTNRTMIRILLAFVSSVGRNDTGYYTCSSSKHPSQSALVTILEKGFINATSSQEEYEIDPYEKFCFSVRFKAYPRIRCTWIFSQASFPCEQRGLEDGYSISKFCDHKNKPGEYIFYAENDDAQFTKMFTLNIRKKPQVLANASASQASCSSDGYPLPSWTWKKCSDKSPNCTEEIPEGVWNKKANRKVFGQWVSSSTLNMSEAGKGLLVKCCAYNSMGTSCETIFLNSPGPFPFIQDNIS

  • Molecular Weight

    Approximately 88 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized human Flt-3 Ligand, at 1 μg/mL (100 μL/well) can bind Biotinylated Mouse FLT3 protein. The ED50 for this effect is 5.987 ng/mL.

  • FLT3 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for FLT3 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for FLT3 Protein, Mouse (HEK293, His)
  • FLT3 Protein, Mouse (HEK293, His) Product Image