FLT3 Protein, Mouse (HEK293, His)

FLT3, a receptor tyrosine kinase, has diverse functions including kinase activation, PI3K binding, and ligase binding. It plays a vital role in hematopoiesis, immune function, and tissue development. FLT3 is expressed in various tissues like the brain, immune system, and reproductive system. It is involved in diseases like leukemia and cancer. The wide functions and tissue-specific expression emphasize FLT3's importance in health and disease. FLT3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FLT3 protein, expressed by HEK293 , with C-His labeled tag.

FLT3, a receptor tyrosine kinase, possesses diverse functional capabilities, including phosphatidylinositol 3-kinase binding, protein tyrosine kinase activity, and ubiquitin protein ligase binding. Its involvement in critical processes such as common myeloid progenitor cell proliferation, hemopoiesis, and lymphocyte proliferation highlights its essential role in hematopoietic development and immune system function. Situated on the external side of the plasma membrane, FLT3 exhibits biased expression in various tissues, including the brain, dorsal aorta, immune and reproductive systems, and spinal cord. This protein serves as a crucial player in diseases like acute myeloid leukemia and juvenile myelomonocytic leukemia, and its orthologs are implicated in conditions such as acute lymphoblastic leukemia, colorectal adenocarcinoma, hepatocellular carcinoma, and prostate cancer. The multifaceted functions and tissue-specific expression underscore the significance of FLT3 in cellular homeostasis and disease progression.

1.This product does not contain protein kinase domain.
2.Measured by its binding ability in a functional ELISA. Immobilized human Flt-3 Ligand, at 1 μg/mL (100 μL/well) can bind Biotinylated Mouse FLT3 protein. The ED50 for this effect is 5.987 ng/mL.

• 
  Measured by its binding ability in a functional ELISA. Immobilized human Flt-3 Ligand, at 1 μg/mL (100 μL/well) can bind Biotinylated Mouse FLT3 protein. The ED₅₀ for this effect is 5.987 ng/mL.

Mouse

HEK293

C-His

Q3UEW6/NP_034359.2 (N28-S544)

14255  [NCBI]

Partial

NQDLPVIKCVLISHENNGSSAGKPSSYRMVRGSPEDLQCAPRRQSEGTVYEAATVEVAESGSITLQVQLATPGDLSCLWVFKHSSLGCQPHFDLQNRGIVSMAILNVTETQAGEYLLHIQSEAANYTVLFTVNVRDTQLYVLRRPYFRKMENQDALLCISEGVPEPTVEWVLCSSHRESCKEEGPAVVRKEEKVLHELFGTDIRCCARNALGRECTKLFTIDLNQAPQSTLPQLFLKVGEPLWIRCKAIHVNHGFGLTWELEDKALEEGSYFEMSTYSTNRTMIRILLAFVSSVGRNDTGYYTCSSSKHPSQSALVTILEKGFINATSSQEEYEIDPYEKFCFSVRFKAYPRIRCTWIFSQASFPCEQRGLEDGYSISKFCDHKNKPGEYIFYAENDDAQFTKMFTLNIRKKPQVLANASASQASCSSDGYPLPSWTWKKCSDKSPNCTEEIPEGVWNKKANRKVFGQWVSSSTLNMSEAGKGLLVKCCAYNSMGTSCETIFLNSPGPFPFIQDNIS

approximately 88-100 kDa

• 
  ≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.