IL1R1 Protein, Human (Cell-Free, His, SUMO, Myc)

IL1R1 protein is the receptor for IL1A, IL1B and IL1RN and forms a high-affinity complex with IL1RAP upon ligand binding. This complex activates NF-kappa-B, MAPK and other pathways, recruiting adapter molecules such as TOLLIP, MYD88 and IRAK1 or IRAK2. IL1R1 Protein, Human (Cell-Free, His, SUMO, Myc) is the recombinant human-derived IL1R1 protein, expressed by E. coli Cell-free , with N-10*His, C-Myc, N-SUMO labeled tag. The total length of IL1R1 Protein, Human (Cell-Free, His, SUMO, Myc) is 552 a.a., with molecular weight of 83.5 kDa.

IL1R1, a receptor for interleukin-1A (IL1A), interleukin-1B (IL1B), and interleukin-1 receptor antagonist (IL1RN), forms a high-affinity complex with the coreceptor IL1RAP upon ligand binding. This complex mediates interleukin-1-dependent activation of signaling pathways such as NF-kappa-B and MAPK. The signaling cascade involves the recruitment of adapter molecules, including TOLLIP, MYD88, and IRAK1 or IRAK2, through the TIR domains of the receptor/coreceptor subunits. IL1R1 binds ligands with comparable affinity, and the interaction with antagonist IL1RN prevents the association with IL1RAP, thus inhibiting the formation of a signaling complex. Furthermore, IL1R1 is implicated in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells.

Human

E. coli Cell-free

N-10*His;C-Myc;N-SUMO

P14778 (L18-G569)

3554

LEADKCKEREEKIILVSSANEIDVRPCPLNPNEHKGTITWYKDDSKTPVSTEQASRIHQHKEKLWFVPAKVEDSGHYYCVVRNSSYCLRIKISAKFVENEPNLCYNAQAIFKQKLPVAGDGGLVCPYMEFFKNENNELPKLQWYKDCKPLLLDNIHFSGVKDRLIVMNVAEKHRGNYTCHASYTYLGKQYPITRVIEFITLEENKPTRPVIVSPANETMEVDLGSQIQLICNVTGQLSDIAYWKWNGSVIDEDDPVLGEDYYSVENPANKRRSTLITVLNISEIESRFYKHPFTCFAKNTHGIDAAYIQLIYPVTNFQKHMIGICVTLTVIIVCSVFIYKIFKIDIVLWYRDSCYDFLPIKASDGKTYDAYILYPKTVGEGSTSDCDIFVFKVLPEVLEKQCGYKLFIYGRDDYVGEDIVEVINENVKKSRRLIIILVRETSGFSWLGGSSEEQIAMYNALVQDGIKVVLLELEKIQDYEKMPESIKFIKQKHGAIRWSGDFTQGPQSAKTRFWKNVRYHMPVQRRSPSSKHQLLSPATKEKLQREAHVPLG

83.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.