RPS13 Protein, Human (GST)

The RPS13 protein is an important component of the multichannel translocon (MPT) complex, which facilitates the insertion of multichannel membrane proteins into lipid bilayers. RPS13 operates downstream of the SEC61 complex, within the PAT subcomplex, and facilitates insertion of the transmembrane region after SEC61 initiation. RPS13 Protein, Human (GST) is the recombinant human-derived RPS13 protein, expressed by E. coli , with N-GST labeled tag. The total length of RPS13 Protein, Human (GST) is 149 a.a., with molecular weight of ~44.0 kDa.

CCDC47 functions as a critical component of the multi-pass translocon (MPT) complex, which plays a pivotal role in the insertion of multi-pass membrane proteins into the lipid bilayer of membranes. Acting downstream of the SEC61 complex, the MPT complex, comprising the PAT subcomplex with CCDC47, facilitates the insertion of subsequent transmembrane regions after the SEC61 complex initiates the process. The PAT subcomplex, in which CCDC47 is involved, shields highly polar regions in transmembrane domains from the non-polar membrane environment until they can be buried in the fully assembled protein. CCDC47 specifically occludes the lateral gate of the SEC61 complex within the PAT subcomplex. Beyond its role in membrane protein insertion, CCDC47 contributes to the regulation of calcium ion homeostasis in the endoplasmic reticulum (ER) and is essential for proper protein degradation via the ER-associated degradation (ERAD) pathway. Furthermore, CCDC47 is indispensable for maintaining ER organization during embryogenesis. As part of the PAT complex, alongside WDR83OS/Asterix, CCDC47 collaborates within the larger MPT complex, which also includes the GEL and BOS subcomplexes. This complex interplay is crucial for efficient protein translocation and ER function. CCDC47 interacts with key proteins such as VCP, HSPA5, DERL1, DERL2, and SELENOS, further emphasizing its significance in cellular processes.

Human

E. coli

N-GST

P62277 (3R-151A)

6207  [NCBI]

RMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIESRIHRLARYYKTKRVLPPNWKYESSTASALVA

Approximately 44.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.