ZZW-115 hydrochloride

Name: ZZW-115 hydrochloride
Brand: MedChemExpress
SKU: HY-111838A
Rating: 4.5 (1 reviews)

Cat. No.: HY-111838A Purity: 98.63%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

ZZW-115 hydrochloride is a potent NUPR1 inhibitor, with a K_d of 2.1 μM. ZZW-115 hydrochloride induces tumor cell death by necroptosis and apoptosis. Anticancer activity.

For research use only. We do not sell to patients.

ZZW-115 hydrochloride Chemical Structure

CAS No. : 10122-45-9

Size	Price	Stock	Quantity
Oil + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 316	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 316	In-stock	Estimated Time of Arrival: December 31
Oil
5 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 380	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 800	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1280	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 2100	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of ZZW-115 hydrochloride:

ZZW-115 Get quote

1 Publications Citing Use of MCE ZZW-115 hydrochloride

•BMC Med. 2022 Oct 19;20(1):365. [Abstract]

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

ZZW-115 hydrochloride is a potent NUPR1 inhibitor, with a K_d of 2.1 μM. ZZW-115 hydrochloride induces tumor cell death by necroptosis and apoptosis. Anticancer activity^[1]^[2].

In Vitro

ZZW-115 hydrochloride (0.1-33 μM; 72 hours) is efficient in killing cancer cells, with an IC₅₀ in the range of 0.84 μM (ANOR) to 4.93 μM (HN14)^[1].
ZZW-115 hydrochloride (0-100 μM; 24-72 hours) is efficient to kill these tumor cells with an IC₅₀ in the range of 0.42 μM (Hep2G cells) to 7.75 μM (SaOS-2 cells)^[1].
ZZW-115 hydrochloride induces pancreatic cell death by necrosis and apoptosis. ZZW-115 hydrochloride treatment induces a decrease in ATP production and induces a ROS overproduction^[1].
LDH release is significantly higher in ZZW-115 hydrochloride-treated cells (MiaPaCa-2, 02-063, LIPC, Foie8b, and HN14 cells) than in control cells in a concentration-dependent manner. Similarly, caspase 3/7 activity is also greater in ZZW-115 hydrochloride-treated cells. These experiments demonstrated that ZZW-115 hydrochloride exerted both pronecrotic and proapoptotic effects^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	ANOR cells, MiaPaCa-2, 02-063, 01008, LIPC, 02136, HN01,01046, AOIPC, Foie8b, HN14 cells
Concentration:	0.1- 33 μM
Incubation Time:	72 hours
Result:	Was efficient in killing cancer cells, with an IC₅₀ in the range of 0.84 μM (ANOR) to 4.93 μM (HN14).

Cell Proliferation Assay^[1]

Cell Line:	U87, A375, U2OS, SaOS-2, HT29, SK-CO-1, LS174T, H1299 and H358, HepG2, PC3, THP-1, Daudi, Jurkat and MDA-MB-231 cells
Concentration:	0-100 μM
Incubation Time:	24 or 72 hours
Result:	Was efficient to kill these tumor cells with an IC₅₀ in the range of 0.42 μM (Hep2G cells) to 7.75 μM (SaOS-2 cells).

In Vivo

ZZW-115 hydrochloride (0.5-5 mg/kg; injection; daily for 30 days) inhibits the growth of pancreatic xenografted tumors^[1].
ZZW-115 hydrochloride (5 mg/kg for 30 days; immunocompetent C57BL/6 mice were orthotopically implanted with Panc02 cells) treatment shows the tumor size is almost unmeasurable in some cases^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	NMRI-Foxn1nu/Foxn1nu mice (nude mice) xenografted with MiaPaCa-2 cells ^[1]
Dosage:	5, 2.5, 1.0, or 0.5 mg/kg
Administration:	Injection; daily for 30 days
Result:	When the mice were injected with 5 mg/kg ZZW-115 hydrochloride, the tumors stopped growing a few days after treatment and their size decreased progressively, almost disappearing at the end of the treatment.

Molecular Weight

573.97

Formula

C₂₄H₃₄Cl₃F₃N₄S

CAS No.

10122-45-9

Appearance

Oil

Color

Light brown to brown

SMILES

FC(C(C=C1N2CCCN3CCN(CCN(C)C)CC3)=CC=C1SC4=C2C=CC=C4)(F)F.[H]Cl.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 83.33 mg/mL (145.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 60 mg/mL (104.54 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7423 mL	8.7113 mL	17.4225 mL
5 mM	0.3485 mL	1.7423 mL	3.4845 mL
10 mM	0.1742 mL	0.8711 mL	1.7423 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 6.25 mg/mL (10.89 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 6.25 mg/mL (10.89 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 50 mg/mL (87.11 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.63%

Select Batch:

Data Sheet (279 KB) SDS (251 KB)

COA (249 KB) RP-HPLC (258 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Santofimia-Castaño P, et al. Ligand-based design identifies a potent NUPR1 inhibitor exerting anticancer activity via necroptosis. J Clin Invest. 2019;129(6):2500-2513. Published 2019 Mar 28. [Content Brief]

[2]. Santofimia-Castaño P, et al. Targeting the Stress-Induced Protein NUPR1 to Treat Pancreatic Adenocarcinoma. Cells. 2019;8(11):1453. Published 2019 Nov 17. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.7423 mL	8.7113 mL	17.4225 mL	43.5563 mL
	5 mM	0.3485 mL	1.7423 mL	3.4845 mL	8.7113 mL
	10 mM	0.1742 mL	0.8711 mL	1.7423 mL	4.3556 mL
	15 mM	0.1162 mL	0.5808 mL	1.1615 mL	2.9038 mL
	20 mM	0.0871 mL	0.4356 mL	0.8711 mL	2.1778 mL
	25 mM	0.0697 mL	0.3485 mL	0.6969 mL	1.7423 mL
	30 mM	0.0581 mL	0.2904 mL	0.5808 mL	1.4519 mL
	40 mM	0.0436 mL	0.2178 mL	0.4356 mL	1.0889 mL
	50 mM	0.0348 mL	0.1742 mL	0.3485 mL	0.8711 mL
	60 mM	0.0290 mL	0.1452 mL	0.2904 mL	0.7259 mL
	80 mM	0.0218 mL	0.1089 mL	0.2178 mL	0.5445 mL
	100 mM	0.0174 mL	0.0871 mL	0.1742 mL	0.4356 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.