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3PO 

Cat. No.: HY-19824 Purity: 98.53%
COA Handling Instructions

3PO is an inhibitor of PFKFB3. 3PO attenuates the proliferation of several cancer cell lines with IC50s of 1.4-24 μmol/L. 3PO suppresses glucose uptake and decreases the intracellular concentration of Fru-2,6-BP, lactate, ATP, NAD+ and NADH. 3PO can be used for the research of cancer.

For research use only. We do not sell to patients.

3PO Chemical Structure

3PO Chemical Structure

CAS No. : 18550-98-6

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply Now  
Solution
10 mM * 1 mL in DMSO USD 73 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 73 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 66 In-stock
Estimated Time of Arrival: December 31
10 mg USD 92 In-stock
Estimated Time of Arrival: December 31
50 mg USD 251 In-stock
Estimated Time of Arrival: December 31
100 mg USD 436 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 13 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

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Description

3PO is an inhibitor of PFKFB3. 3PO attenuates the proliferation of several cancer cell lines with IC50s of 1.4-24 μmol/L. 3PO suppresses glucose uptake and decreases the intracellular concentration of Fru-2,6-BP, lactate, ATP, NAD+ and NADH. 3PO can be used for the research of cancer[1].

In Vitro

3PO (0-33 μM; 0-36 h) inhibits Jurkat cells proliferation by influence cell cycle[1].
3PO (10 μM/L; 0-36 h) suppresses glycolytic flux to lactate in Jurkat cells[1].
3PO (10 μM/L; 0-36 h) inhibits cellular proliferation of transformed tumor cell lines[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[1]

Cell Line: Jurkat cells
Concentration: 0.3, 1, 3, 10 and 33 μM
Incubation Time: 0, 4, 8, 16, 24 and 36 hours
Result: Inhibited Jurkat T cells proliferation by G2-M phase cell cycle arrest.

Cell Viability Assay[1]

Cell Line: Jurkat cells
Concentration: 0.3, 1, 3, 10 and 33 μM
Incubation Time: 0, 4, 8, 16, 24 and 36 hours
Result: Decreased lactate secretion, NADH, NAD+ and ATP in Jurkat cells.

Cell Proliferation Assay[1]

Cell Line: Jurkat, K562, HL-60, MDA-MB231, HL-60, HeLa, Melanoma and Lewis Lung
Concentration: 0-20 μM
Incubation Time: 0, 4, 8, 16, 24 and 36 hours
Result: Inhibited solid tumor and hematologic cell lines with IC50s of 1.4-24 μM/L.
In Vivo

3PO (0.07 mg/g; i.p. once per day for 14 days) inhibits the growth of Lewis lung carcinoma xenografts in C57Bl/6 mice[1].
3PO (0.07 mg/g; i.p. three sequential daily injections followed by 3 off days for 14 days) inhibits the tumor growth in BALB/c athymic mice[1].
3PO (0.07 mg/g; i.p. two daily injections followed a 7-day rest for 14 days) inhibits the tumor growth in BALB/c athymic mice[1].
3PO (0.07 mg/g; i.p. once) inhibits Fru-2,6-BP and glucose uptake in C57Bl/6 mice[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female C57Bl/6 mice with Lewis lung carcinoma xenografts[1]
Dosage: 0.07 mg/g
Administration: Intraperitoneal injection; 0.07 mg/g once a day; for 14 days
Result: Suppressed the growth of Lewis lung carcinoma xenografts in C57Bl/6 mice compared to the control group.
Animal Model: Female BALB/c athymic mice with MDA-MB231 human breast adenocarcinoma xenografts[1]
Dosage: 0.07 mg/g
Administration: Intraperitoneal injection; 0.07 mg/g three sequential daily injections of either DMSO or 3PO followed by 3 off days; for 14 days
Result: Suppressed xenograft tumorigenic growth of MDA-MB231 cells compared with the controls.
Animal Model: Female BALB/c athymic mice with HL-60 leukemia xenografts[1]
Dosage: 0.07 mg/g
Administration: Intraperitoneal injection; 0.07 mg/g two daily injections followed a 7-day rest; for 14 days
Result: Suppressed xenograft tumorigenic growth of HL-60 leukemia compared with the controls, especially from the second administration cycle.
Animal Model: Female C57Bl/6 mice with Lewis lung carcinoma xenografts[1]
Dosage: 0.07 mg/g
Administration: Intraperitoneal injection; once
Result: Decreased Fru-2,6-BP and glucose uptake in C57Bl/6 mice compared to control group.
Molecular Weight

210.23

Formula

C13H10N2O

CAS No.
SMILES

O=C(C1=CC=NC=C1)/C=C/C2=CC=CN=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 60 mg/mL (285.40 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.7567 mL 23.7835 mL 47.5670 mL
5 mM 0.9513 mL 4.7567 mL 9.5134 mL
10 mM 0.4757 mL 2.3783 mL 4.7567 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  50% PEG300    50% saline

    Solubility: 6 mg/mL (28.54 mM); Clear solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 3 mg/mL (14.27 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 3 mg/mL (14.27 mM); Clear solution

  • 4.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 3 mg/mL (14.27 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
3PO
Cat. No.:
HY-19824
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