Alda-1
Based on 18 publication(s) in Google Scholar
Alda-1 is a potent and selective ALDH2 agonist, which activates wild-type ALDH2 and restores near wild-type activity to ALDH2*2.
For research use only. We do not sell to patients.
- Purity: 99.99%
- CAS No.: 349438-38-6
- Formula: C15H11Cl2NO3
- Molecular Weight:324.16
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Alda-1
More- Circulation. 2024 May 28;149(22):1752-1769. [Abstract]
- Carbohydr Polym. 2022 Jul 1:287:119326. [Abstract]
- Cell Death Dis. 2025 Aug 13;16(1):616. [Abstract]
- Cell Death Dis. 2023 Jan 20;14(1):45. [Abstract]
- Free Radic Biol Med. 2024 Feb 20:212:34-48. [Abstract]
- Neoplasia. 2019 Jun;21(6):602-614. [Abstract]
- Arterioscler Thromb Vasc Biol. 2019 Nov;39(11):2303-2319. [Abstract]
- Anal Chem. 2022 Jan 18;94(2):1308-1317. [Abstract]
- Life Sci. 2018 Dec 15:215:227-235. [Abstract]
- J Inflamm Res. 2025 Aug 14:18:11095-11108. [Abstract]
- J Immunol Res. 2018 Dec 24:2018:9807139. [Abstract]
- Food Chem Toxicol. 2023 Dec:182:114129. [Abstract]
- Mol Med Rep. 2020 Sep;22(3):2373-2385. [Abstract]
- Exp Biol Med. 2021 Aug;246(15):1740-1749. [Abstract]
- Biochem Biophys Res Commun. 2020 Dec 17;533(4):1427-1434. [Abstract]
- Biochem Biophys Res Commun. 2020 Feb 5;522(2):518-524. [Abstract]
- Neurosci Lett. 2018 May 1:674:156-161. [Abstract]
- University of Sao Paulo. 2022.
Customer Validation & Images
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Biological Activity
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ALDH2 |
Alda-1 (1.5 mg/kg, b.w., IP) administration significantly increases the climbing time, tends to reduce the immobility time and increases the swimming time of the prenatally stressed rats in the forced swim test. Moreover, treatment of prenatally stressed rats with Alda-1 significantly increases number of entries into the open arms of the maze and the time spent therein, as assessed by elevated plus-maze test[2]. Alda-1 (8.5 mg/kg; IP) with glucose significantly lowers 4-HNE and FJB-positive cells in the cerebral cortex of Alda-1-treated rats than in DMSO-treated rats 24 h after glucose administration[3].
Alda-1 (10 mg/kg per day) treatment prevents aldehydic overload, mitochondrial dysfunction and improves ventricular function in post-MI cardiomyopathy rats[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 349438-38-6
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Appearance Solid
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Molecular Weight 324.16
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Formula C15H11Cl2NO3
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Color White to off-white
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SMILES
O=C(NCC1=CC=C(OCO2)C2=C1)C3=C(Cl)C=CC=C3Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (18)
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Journal Impact Factor
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Most Recent
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Circulation
2024 May 28;149(22):1752-1769. PMID: 38348663 -
Carbohydr Polym
Antibacterial and antioxidant chitosan nanoparticles improve the preservation effect for donor kidneys in vitro. [Abstract]2022 Jul 1:287:119326. PMID: 35422292 -
Cell Death Dis
The ALDH2/PolG2 axis enhances mitochondrial biogenesis via transcriptional regulation of Nrf2 and promotes chemotherapy resistance in acute myeloid leukaemia. [Abstract]2025 Aug 13;16(1):616. PMID: 40796730 -
Cell Death Dis
Aldehyde dehydrogenase 2 alleviates mitochondrial dysfunction by promoting PGC-1α-mediated biogenesis in acute kidney injury. [Abstract]2023 Jan 20;14(1):45. PMID: 36670098 -
Free Radic Biol Med
2024 Feb 20:212:34-48. PMID: 38104741 -
Neoplasia
ALDH2 Repression Promotes Lung Tumor Progression via Accumulated Acetaldehyde and DNA Damage. [Abstract]2019 Jun;21(6):602-614. PMID: 31071657 -
Arterioscler Thromb Vasc Biol
ALDH2 (Aldehyde Dehydrogenase 2) Protects Against Hypoxia-Induced Pulmonary Hypertension. [Abstract]2019 Nov;39(11):2303-2319. PMID: 31510791 -
Anal Chem
In Situ Imaging of Formaldehyde in Live Mice with High Spatiotemporal Resolution Reveals Aldehyde Dehydrogenase-2 as a Potential Target for Alzheimer's Disease Treatment. [Abstract]2022 Jan 18;94(2):1308-1317. PMID: 34962779 -
Life Sci
ALDH2 attenuates early-stage STZ-induced aged diabetic rats retinas damage via Sirt1/Nrf2 pathway. [Abstract]2018 Dec 15:215:227-235. PMID: 30315856
Alda-1 purchased from MedChemExpress. Usage Cited in: Life Sci. 2018 Dec 15:215:227-235. [Abstract]
VEGF-α representative western blot and relative density of immunoblot in DMSO group and Alda1-treated group at 1 d, 7 d and 30 d post aged diabetic rats model established.
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J Inflamm Res
ALDH2 Ameliorates Acute Gouty Arthritis Through Inhibiting NLRP3 Inflammasome and Pyroptosis by Nrf2/ROS Pathway. [Abstract]2025 Aug 14:18:11095-11108. PMID: 40831518 -
J Immunol Res
Alda-1 Ameliorates Liver Ischemia-Reperfusion Injury by Activating Aldehyde Dehydrogenase 2 and Enhancing Autophagy in Mice. [Abstract]2018 Dec 24:2018:9807139. PMID: 30671488
Alda-1 purchased from MedChemExpress. Usage Cited in: J Immunol Res. 2018 Dec 24:2018:9807139. [Abstract]
Western blot assays show that 3-MA abrogates the Alda-1 induced expression increases in LC3BⅡ and Bcl2 and decreases in P62 and Bax.
Alda-1 purchased from MedChemExpress. Usage Cited in: J Immunol Res. 2018 Dec 24:2018:9807139. [Abstract]
Western blot analysis of p-AMPK, AMPK, Bcl2 and Bax, LC3B and P62 expression in liver tissues.
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Food Chem Toxicol
Deficiency of aldehyde dehydrogenase 2 aggravates ethanol-induced cytotoxicity in N2a cells via CaMKII/Drp1-mediated mitophagy. [Abstract]2023 Dec:182:114129. PMID: 37967785 -
Mol Med Rep
Pretreatment with the ALDH2 activator Alda‑1 protects rat livers from ischemia/reperfusion injury by inducing autophagy. [Abstract]2020 Sep;22(3):2373-2385. PMID: 32705206 -
Exp Biol Med
Protective effect of aldehyde dehydrogenase 2 against rat corneal dysfunction caused by streptozotocin-induced type I diabetes. [Abstract]2021 Aug;246(15):1740-1749. PMID: 33969723 -
Biochem Biophys Res Commun
Aldehyde dehydrogenase 2 protects against sympathetic excitation-induced cardiac fibrosis. [Abstract]2020 Dec 17;533(4):1427-1434. PMID: 33333711 -
Biochem Biophys Res Commun
A small-molecule activator of mitochondrial aldehyde dehydrogenase 2 reduces the severity of cerulein-induced acute pancreatitis. [Abstract]2020 Feb 5;522(2):518-524. PMID: 31784085 -
Neurosci Lett
The impact of ALDH2 activation by Alda-1 on the expression of VEGF in the hippocampus of a rat model of post-MI depression. [Abstract]2018 May 1:674:156-161. PMID: 29574217 -
Solvent & Solubility
DMSO : 100 mg/mL (308.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
Spleen cells (4×106 cells/mL) are stimulated by optimal concentrations of concanavalin A (Con A; 2.5 μg/mL and 0.6 μg/mL) and lipopolysaccharide (LPS, 5 μg/mL) and are incubated in 96-well plates at final volume of 0.2 mL for 72 h. Cell proliferation is determined by adding 0.5 μCi of [3H]-thymidine per well at 16 h before the end of the incubation. The cultures are harvested with an automatic cell harvester, and [3H] thymidine incorporation is assessed using a liquid scintillation counter.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
After behavioral verification at three months of age, the animals are divided into the following four groups: control, control + Alda-1, prenatally stressed and prenatally stressed + Alda-1 (6 animals per group). Alda-1 injections are given intraperitoneally (i.p.) once daily at a dose of 1.5 mg/kg b.w. (dissolved in 1 mL/kg b.w. DMSO/water 50/50) for 14 days. At the same time, the control and prenatally stressed rats receive 1 mL/kg b.w. DMSO/water 50/50. The injections of Alda-1 and vehicle are given between 10 a.m and 11 a.m. In the last five days of Alda-1 treatment the behavioral parameters in the elevated plus maze test and then in the forced swim test are measured.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (623 KB)
- English - EN (623 KB)
- Français - FR (623 KB)
- Deutsch - DE (623 KB)
- Norwegian - NO (623 KB)
- Español - ES (623 KB)
- Swedish - SV (623 KB)
- Italian - IT (623 KB)
- Portuguese - PT (623 KB)
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Handling Instructions (2659 KB)
References
[1]. Stachowicz A, et al. Proteomic Analysis of Mitochondria-Enriched Fraction Isolated from the Frontal Cortex and Hippocampus of Apolipoprotein E Knockout Mice Treated with Alda-1, an Activator of Mitochondrial Aldehyde Dehydrogenase (ALDH2). Int J Mol Sci. [Content Brief]
[2]. Stachowicz A, et al. The impact of mitochondrial aldehyde dehydrogenase (ALDH2) activation by Alda-1 on the behavioral and biochemical disturbances in animal model of depression. Brain Behav Immun. 2016 Jan;51:144-53. [Content Brief]
[3]. Ikeda T, et al. Effects of Alda-1, an Aldehyde Dehydrogenase-2 Agonist, on Hypoglycemic Neuronal Death. PLoS One. 2015 Jun 17;10(6):e0128844. [Content Brief]
[4]. Gomes KM, et al. Aldehydic load and aldehyde dehydrogenase 2 profile during the progression of post-myocardial infarction cardiomyopathy: benefits of Alda-1. Int J Cardiol. 2015 Jan 20;179:129-138. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0849 mL | 15.4245 mL | 30.8490 mL | 77.1224 mL |
| 5 mM | 0.6170 mL | 3.0849 mL | 6.1698 mL | 15.4245 mL | |
| 10 mM | 0.3085 mL | 1.5424 mL | 3.0849 mL | 7.7122 mL | |
| 15 mM | 0.2057 mL | 1.0283 mL | 2.0566 mL | 5.1415 mL | |
| 20 mM | 0.1542 mL | 0.7712 mL | 1.5424 mL | 3.8561 mL | |
| 25 mM | 0.1234 mL | 0.6170 mL | 1.2340 mL | 3.0849 mL | |
| 30 mM | 0.1028 mL | 0.5141 mL | 1.0283 mL | 2.5707 mL | |
| 40 mM | 0.0771 mL | 0.3856 mL | 0.7712 mL | 1.9281 mL | |
| 50 mM | 0.0617 mL | 0.3085 mL | 0.6170 mL | 1.5424 mL | |
| 60 mM | 0.0514 mL | 0.2571 mL | 0.5141 mL | 1.2854 mL | |
| 80 mM | 0.0386 mL | 0.1928 mL | 0.3856 mL | 0.9640 mL | |
| 100 mM | 0.0308 mL | 0.1542 mL | 0.3085 mL | 0.7712 mL |
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