1. Cell Cycle/DNA Damage
  2. DNA/RNA Synthesis

Didox (Synonyms: N,3,4-Trihydroxybenzamide)

Cat. No.: HY-19387 Purity: 98.72%
Handling Instructions

Didox is a synthetic ribonucleotide reductase (RR) inhibitor.

For research use only. We do not sell to patients.

Didox Chemical Structure

Didox Chemical Structure

CAS No. : 69839-83-4

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10 mM * 1 mL in DMSO USD 73 In-stock
Estimated Time of Arrival: December 31
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10 mg USD 96 In-stock
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50 mg USD 420 In-stock
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100 mg USD 720 In-stock
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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Didox is a synthetic ribonucleotide reductase (RR) inhibitor.

IC50 & Target

Ribonucleotide reductase[1]

In Vitro

Didox suppresses LPS-induced mRNA levels of iNOS, IL-6, IL-1, TNF-α, NF-κβ (p65), and p38-α, after 24 h of treatment. Treatment with Didox also suppresses the secretion of nitric oxide (NO), IL-6, and IL-10. Using mitochondrial dehydrogenase activity as a measure of cytotoxicity, the effects of Didox on cellular respiration in RAW264.7 are examined over a range of concentrations for 24 h. Cells exposures to 200 μM and below Didox, with and without LPS, do not exhibit significant cellular toxicity[1]. Didox is active against all human and murine acute myeloid leukemia (AML) lines tested with IC50 values in the low micromolar range (mean IC50 37 µM [range 25.89-52.70 µM])[2].

In Vivo

Once engraftment is established by bioluminescent imaging, the animals receive daily administrations of Didox at 425 mg/kg via IP injection over 5 days. Didox treatment significantly reduces leukemic burden compared to vehicle treated controls (p=0.0026 and p=0.0342). More importantly, Didox provides a significant survival benefit (p<0.0001 and p=0.0094)[2].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.9126 mL 29.5631 mL 59.1261 mL
5 mM 1.1825 mL 5.9126 mL 11.8252 mL
10 mM 0.5913 mL 2.9563 mL 5.9126 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

RAW264.7 macrophages are treated with Didox alone, with 0.1 μg/mL LPS, or the two in combination. Cellular respiration, as an indication of cytotoxicity, is measured by the MTT assay, which quantifies mitochondrial dehydrogenase activity. Macrophages are plated into 96 well Costar plates at 105 cells per well in 100 μL of DMEM media. After 4 h of incubation at 37°C for adherence, compounds and DMSO carrier control (0.01% final) are added in triplicate over serial dilutions beginning with 200 μM per well in a total volume of 200 μL, and the plates incubated for 24 h. Four h before termination of the assay, each well receives 20 μL of a 5 mg/mL MTT solution in un-supplemented DMEM. After centrifugation, the supernatant for each well is discarded and cells containing reduced MTT are solubilized with 100 μL of acidified isopropanol (4 mM HCl, 0.1% NP-40 in isopropanol). Following a brief period of shaking, the optical density (O.D.) for each well is recorded at 550 nm. Each experiment is repeated three times and the data averaged from each triplicate, then expressed as percentage of the control O.D. values for each experiment[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

Mice[2]
Luciferase-tagged leukemia cells are transplanted into 8- week old, sublethally irradiated (4.5 Gy) C57Bl/6 mice by tail vein injection of 1.0×106 cells per mouse. Mice are injected with 150 mg/kg D-Luciferin, anesthetised with Isoflurane, and imaged using the IVIS 100 imaging system. Mice begin treatment with Didox upon detection of clear signal. The animals are treated with daily administrations of Didox at 425 mg/kg Didox by intraperitoneal injection (IP) for 5 days. Control animals receive 5% dextrose water by IP injection. Repeat imaging is performed on the day following the final treatment[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

169.13

Formula

C₇H₇NO₄

CAS No.

69839-83-4

SMILES

O=C(NO)C1=CC=C(O)C(O)=C1

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
Didox
Cat. No.:
HY-19387
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Didox

Cat. No.: HY-19387