I-BRD9

Name: I-BRD9
Brand: MedChemExpress
SKU: HY-18975
Rating: 5.0 (9 reviews)

Cat. No.: HY-18975 Purity: 99.72%: Data Sheet
COA

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Handling Instructions
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I-BRD9 is a selective cellular chemical probe of bromodomain-containing protein 9 (BRD9) with pIC₅₀ value of 7.3 μM. I-BRD9 has high selectivity for bromodomain and extra terminal domain (BET) family and highly homologous bromodomain-containing protein 7 (BRD7). I-BRD9 can be used to identify genes regulated by BRD9 in Kasumi-1 cells involved in oncology and immune response pathways.

For research use only. We do not sell to patients.

CAS No. : 1714146-59-4

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 9 publication(s) in Google Scholar

9 Publications Citing Use of MCE I-BRD9

RT-PCR

Cell Proliferation/Viability Assay

In Vivo Efficacy Study

Cell Imaging/Staining

: I-BRD9 purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Apr 25;11(17):eads6385. [Abstract]; IB analysis of WCL derived for MDA-MB-231 cells treated with 5 μM I-BRD9 for the indicated time.

: I-BRD9 purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Apr 25;11(17):eads6385. [Abstract]; RT-qPCR analysis of mRNA levels of select genes commonly regulated by I-BRD9 and ipatasertib. MDA-MB-231 cells were treated with 5 μM I-BRD9 or 1 μM ipatasertib for 16 hours.

: I-BRD9 purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Apr 25;11(17):eads6385. [Abstract]; MDA-MB-231 cells treated with I-BRD9 (5 μM) or GSK126 were subjected to cell proliferation assays.

: I-BRD9 purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Apr 25;11(17):eads6385. [Abstract]; Tumor growth curve upon treatment of I-BRD9 (30 mg/kg) and GSK126.

: I-BRD9 purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Apr 25;11(17):eads6385. [Abstract]; MDA-MB-231 cells treated with I-BRD9 (5 μM) or GSK126 were subjected to colony formation assays.

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Biological Activity
Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
G-401	IC₅₀	0.008 μM Compound: Chemical probe: I-BRD9	Anti-proliferative activity against human G-401 cells assessed as inhibition cell growth incubated for 72 hrs in presence of vincristine by MTT-based Chou-Talalay method Anti-proliferative activity against human G-401 cells assessed as inhibition cell growth incubated for 72 hrs in presence of vincristine by MTT-based Chou-Talalay method	[PMID: 28714904]
G-401	IC₅₀	0.096 μM Compound: Chemical probe: I-BRD9	Anti-proliferative activity against human G-401 cells assessed as inhibition cell growth incubated for 72 hrs in presence of doxorubicin by MTT-based Chou-Talalay method Anti-proliferative activity against human G-401 cells assessed as inhibition cell growth incubated for 72 hrs in presence of doxorubicin by MTT-based Chou-Talalay method	[PMID: 28714904]
G-401	IC₅₀	13.4 μM Compound: Chemical probe: I-BRD9	Anti-proliferative activity against human G-401 cells assessed as cell viability incubated for 72 hrs by MTT assay Anti-proliferative activity against human G-401 cells assessed as cell viability incubated for 72 hrs by MTT assay	[PMID: 28714904]
G-401	IC₅₀	6.1 μM Compound: Chemical probe: I-BRD9	Anti-proliferative activity against human G-401 cells assessed as cell viability incubated for 144 hrs by MTT assay Anti-proliferative activity against human G-401 cells assessed as cell viability incubated for 144 hrs by MTT assay	[PMID: 28714904]
G-401	IC₅₀	9.1 μM Compound: Chemical probe: I-BRD9	Anti-proliferative activity against human G-401 cells assessed as inhibition cell growth incubated for 72 hrs in presence of carboplatin by MTT-based Chou-Talalay method Anti-proliferative activity against human G-401 cells assessed as inhibition cell growth incubated for 72 hrs in presence of carboplatin by MTT-based Chou-Talalay method	[PMID: 28714904]
HL-60	GI₅₀	0.433 μM Compound: Chemical probe: I-BRD9	Antiproliferative activity against human HL-60 cells assessed as inhibition of cell growth for 7 days by cell titre glo assay Antiproliferative activity against human HL-60 cells assessed as inhibition of cell growth for 7 days by cell titre glo assay	[PMID: 27376689]
Jurkat	GI₅₀	3.643 μM Compound: Chemical probe: I-BRD9	Antiproliferative activity against human Jurkat cells assessed as inhibition of cell growth for 7 days by cell titre glo assay Antiproliferative activity against human Jurkat cells assessed as inhibition of cell growth for 7 days by cell titre glo assay	[PMID: 27376689]

In Vitro

I-BRD9 (10 μM, 6 h) has a regulation role in cancer and immunology pathways^[1].
I-BRD9 (10 μM) has high BRD9 affinity and excellent broader bromodomain selectivity^[1].
I-BRD9 (10 μM) has affinity for BRD9 (TR-FRET), BRD4 BD1 (TR-FRET) and BRD9 NanoBRET with pIC₅₀ values of 7.3 μM, 5.3 μM and 6.8 μM^[1].
I-BRD9 (10 μM) has high aqueous solubility (CLND) with 359 μM^[1].
I-BRD9 (10 μM) displays >625 fold selectivity in binding with endogenous BRD9 against BET family member BRD3 in HUT-78 cell lysate^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:	Kasumi-1 cells
Concentration:	10 μM
Incubation Time:	6 h
Result:	Showed 4 genes (CLEC1, 62 DUSP6, 63 FES64 and SAMSN165) that were strongly down-regulated and not by I-BET151.

Molecular Weight

497.55

Formula

C₂₂H₂₂F₃N₃O₃S₂

CAS No.

1714146-59-4

Appearance

Solid

Color

White to off-white

SMILES

N=C(C(S1)=CC2=C1C(C3=CC=CC(C(F)(F)F)=C3)=CN(CC)C2=O)NC(CC4)CCS4(=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 13.89 mg/mL (27.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0098 mL	10.0492 mL	20.0985 mL
5 mM	0.4020 mL	2.0098 mL	4.0197 mL
10 mM	0.2010 mL	1.0049 mL	2.0098 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.39 mg/mL (2.79 mM); Clear solution

This protocol yields a clear solution of ≥ 1.39 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (13.9 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.39 mg/mL (2.79 mM); Clear solution

This protocol yields a clear solution of ≥ 1.39 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (13.9 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.39 mg/mL (2.79 mM); Clear solution

This protocol yields a clear solution of ≥ 1.39 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (13.9 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.72%

Select Batch:

Data Sheet (275 KB) SDS (480 KB)

COA (251 KB) HNMR (236 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Natalie H Theodoulou, et al. Discovery of I-BRD9, a Selective Cell Active Chemical Probe for Bromodomain Containing Protein 9 Inhibition. J Med Chem [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0098 mL	10.0492 mL	20.0985 mL	50.2462 mL
	5 mM	0.4020 mL	2.0098 mL	4.0197 mL	10.0492 mL
	10 mM	0.2010 mL	1.0049 mL	2.0098 mL	5.0246 mL
	15 mM	0.1340 mL	0.6699 mL	1.3399 mL	3.3497 mL
	20 mM	0.1005 mL	0.5025 mL	1.0049 mL	2.5123 mL
	25 mM	0.0804 mL	0.4020 mL	0.8039 mL	2.0098 mL

I-BRD9 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

I-BRD91714146-59-4Epigenetic Reader Domainprobebromodomain and extra terminal domain (BET)bromodomain-containing protein 7(BRD7)Kasumi-1 cellsBRD9cancerimmune responseInhibitorinhibitorinhibit

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I-BRD9

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I-BRD9 Related Antibodies

9 Publications Citing Use of MCE I-BRD9

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I-BRD9 Related Antibodies

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Complete Stock Solution Preparation Table

I-BRD9 Related Classifications

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