1. Epigenetics
  2. Epigenetic Reader Domain
  3. I-BRD9

I-BRD9 

Cat. No.: HY-18975 Purity: 99.79%
Handling Instructions

I-BRD9 is a selective cellular chemical probe of bromodomain-containing protein 9 (BRD9) with pIC50 value of 7.3 μM. I-BRD9 has high selectivity for bromodomain and extra terminal domain (BET) family and highly homologous bromodomain-containing protein 7 (BRD7). I-BRD9 can be used to identify genes regulated by BRD9 in Kasumi-1 cells involved in oncology and immune response pathways.

For research use only. We do not sell to patients.

I-BRD9 Chemical Structure

I-BRD9 Chemical Structure

CAS No. : 1714146-59-4

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 109 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 109 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 99 In-stock
Estimated Time of Arrival: December 31
10 mg USD 165 In-stock
Estimated Time of Arrival: December 31
25 mg USD 352 In-stock
Estimated Time of Arrival: December 31
50 mg USD 627 In-stock
Estimated Time of Arrival: December 31
100 mg USD 1100 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE I-BRD9

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

I-BRD9 is a selective cellular chemical probe of bromodomain-containing protein 9 (BRD9) with pIC50 value of 7.3 μM. I-BRD9 has high selectivity for bromodomain and extra terminal domain (BET) family and highly homologous bromodomain-containing protein 7 (BRD7). I-BRD9 can be used to identify genes regulated by BRD9 in Kasumi-1 cells involved in oncology and immune response pathways[1].

In Vitro

I-BRD9 (10 μM, 6 h) has a regulation role in cancer and immunology pathways[1].
I-BRD9 (10 μM) has high BRD9 affinity and excellent broader bromodomain selectivity[1].
I-BRD9 (10 μM) has affinity for BRD9 (TR-FRET), BRD4 BD1 (TR-FRET) and BRD9 NanoBRET with pIC50 values of 7.3 μM, 5.3 μM and 6.8 μM[1].
I-BRD9 (10 μM) has high aqueous solubility (CLND) with 359 μM[1].
I-BRD9 (10 μM) displays >625 fold selectivity in binding with endogenous BRD9 against BET family member BRD3 in HUT-78 cell lysate[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR[1]

Cell Line: Kasumi-1 cells
Concentration: 10 μM
Incubation Time: 6 h
Result: Showed 4 genes (CLEC1, 62 DUSP6, 63 FES64 and SAMSN165) that were strongly down-regulated and not by I-BET151.
Molecular Weight

497.55

Formula

C22H22F3N3O3S2

CAS No.
SMILES

N=C(C(S1)=CC2=C1C(C3=CC=CC(C(F)(F)F)=C3)=CN(CC)C2=O)NC(CC4)CCS4(=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 13.89 mg/mL (27.92 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0098 mL 10.0492 mL 20.0985 mL
5 mM 0.4020 mL 2.0098 mL 4.0197 mL
10 mM 0.2010 mL 1.0049 mL 2.0098 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 1.39 mg/mL (2.79 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 1.39 mg/mL (2.79 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 1.39 mg/mL (2.79 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.79%

References
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Product Name:
I-BRD9
Cat. No.:
HY-18975
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