1. Cell Cycle/DNA Damage
  2. Deubiquitinase
  3. ML364

ML364 

Cat. No.: HY-100900 Purity: 99.94%
Handling Instructions

ML364 is a selective ubiquitin specific peptidase 2 (USP2) inhibitor (IC50=1.1 μM) with anti-proliferative activity, which direct binds to USP2 (Kd=5.2 μM), induces an increase in cellular cyclin D1 degradation and causes cell cycle arrest. ML364 increases the levels of mitochondrial ROS and decreases in the intracellular content of ATP.

For research use only. We do not sell to patients.

ML364 Chemical Structure

ML364 Chemical Structure

CAS No. : 1991986-30-1

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10 mM * 1 mL in DMSO USD 85 In-stock
Estimated Time of Arrival: December 31
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10 mg USD 120 In-stock
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50 mg USD 520 In-stock
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100 mg USD 880 In-stock
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Customer Review

Based on 3 publication(s) in Google Scholar

Top Publications Citing Use of Products

    ML364 purchased from MCE. Usage Cited in: Physiol Rep. 2019 Jul;7(14):e14193. 

    Effects of ML364 on cytotoxicity, proliferation, intracellular level of ATP, mitochondrial membrane potential, mitochondria morphology, and accumulation of ROS in C2C12 cells.
    • Biological Activity

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    Description

    ML364 is a selective ubiquitin specific peptidase 2 (USP2) inhibitor (IC50=1.1 μM) with anti-proliferative activity, which direct binds to USP2 (Kd=5.2 μM), induces an increase in cellular cyclin D1 degradation and causes cell cycle arrest. ML364 increases the levels of mitochondrial ROS and decreases in the intracellular content of ATP[1][2].

    IC50 & Target

    IC50: 1.1 μM (USP2)[1]
    Kd: 5.2 μM (USP2)[1]

    In Vitro

    ML364 (5-20 μM; 24-48 hours) inhibits LnCAP and MCF7 cells viability in a dose-dependent manner[1].
    ML364 (10 μM; 2-24 hours) reduces cyclin D1 protein levels in a time-, dose-, and proteasome-dependent manner in HCT116 cells and Mino cells[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[1]

    Cell Line: LnCAP, MCF7 cells
    Concentration: 5, 10, 15, 20 μM
    Incubation Time: 24, 48 hours
    Result: LnCAP and MCF7 cells showed a decrease in cell viability in a dose-dependent manner.

    Western Blot Analysis[1]

    Cell Line: HCT116, Mino cells
    Concentration: 2, 4, 8, 16, 24 hours
    Incubation Time: 10 μM
    Result: Reduced cyclin D1 protein levels in a time-, dose-, and proteasome-dependent manner in HCT116 cells and Mino cells.
    Molecular Weight

    517.54

    Formula

    C₂₄H₁₈F₃N₃O₃S₂

    CAS No.

    1991986-30-1

    SMILES

    O=C(NC1=NC(C2=CC=CC=C2)=CS1)C3=CC=C(C(F)(F)F)C=C3NS(=O)(C4=CC=C(C)C=C4)=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 33 mg/mL (63.76 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9322 mL 9.6611 mL 19.3222 mL
    5 mM 0.3864 mL 1.9322 mL 3.8644 mL
    10 mM 0.1932 mL 0.9661 mL 1.9322 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  0.5% CMC-Na/saline water

      Solubility: 20 mg/mL (38.64 mM); Suspended solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (4.83 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (4.83 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
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    Keywords:

    ML364ML 364ML-364DeubiquitinaseDUBsInhibitorinhibitorinhibit

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    Product Name:
    ML364
    Cat. No.:
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