1. Cell Cycle/DNA Damage
  2. Deubiquitinase
  3. ML364

ML364 

Cat. No.: HY-100900 Purity: 99.94%
COA Handling Instructions

ML364 is a selective ubiquitin specific peptidase 2 (USP2) inhibitor (IC50=1.1 μM) with anti-proliferative activity, which direct binds to USP2 (Kd=5.2 μM), induces an increase in cellular cyclin D1 degradation and causes cell cycle arrest. ML364 increases the levels of mitochondrial ROS and decreases in the intracellular content of ATP.

For research use only. We do not sell to patients.

ML364 Chemical Structure

ML364 Chemical Structure

CAS No. : 1991986-30-1

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 94 In-stock
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10 mM * 1 mL in DMSO USD 94 In-stock
Solid
5 mg USD 83 In-stock
10 mg USD 132 In-stock
50 mg USD 572 In-stock
100 mg USD 968 In-stock
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Customer Review

Based on 12 publication(s) in Google Scholar

Top Publications Citing Use of Products

    ML364 purchased from MedChemExpress. Usage Cited in: Physiol Rep. 2019 Jul;7(14):e14193.   [Abstract]

    Effects of ML364 on cytotoxicity, proliferation, intracellular level of ATP, mitochondrial membrane potential, mitochondria morphology, and accumulation of ROS in C2C12 cells.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    ML364 is a selective ubiquitin specific peptidase 2 (USP2) inhibitor (IC50=1.1 μM) with anti-proliferative activity, which direct binds to USP2 (Kd=5.2 μM), induces an increase in cellular cyclin D1 degradation and causes cell cycle arrest. ML364 increases the levels of mitochondrial ROS and decreases in the intracellular content of ATP[1][2].

    IC50 & Target

    IC50: 1.1 μM (USP2)[1]
    Kd: 5.2 μM (USP2)[1]

    In Vitro

    ML364 (5-20 μM; 24-48 hours) inhibits LnCAP and MCF7 cells viability in a dose-dependent manner[1].
    ? ML364 (10 μM; 2-24 hours) reduces cyclin D1 protein levels in a time-, dose-, and proteasome-dependent manner in HCT116 cells and Mino cells[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[1]

    Cell Line: LnCAP, MCF7 cells
    Concentration: 5, 10, 15, 20 μM
    Incubation Time: 24, 48 hours
    Result: LnCAP and MCF7 cells showed a decrease in cell viability in a dose-dependent manner.

    Western Blot Analysis[1]

    Cell Line: HCT116, Mino cells
    Concentration: 2, 4, 8, 16, 24 hours
    Incubation Time: 10 μM
    Result: Reduced cyclin D1 protein levels in a time-, dose-, and proteasome-dependent manner in HCT116 cells and Mino cells.
    Molecular Weight

    517.54

    Formula

    C24H18F3N3O3S2

    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C(NC1=NC(C2=CC=CC=C2)=CS1)C3=CC=C(C(F)(F)F)C=C3NS(=O)(C4=CC=C(C)C=C4)=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 33 mg/mL (63.76 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9322 mL 9.6611 mL 19.3222 mL
    5 mM 0.3864 mL 1.9322 mL 3.8644 mL
    10 mM 0.1932 mL 0.9661 mL 1.9322 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  0.5% CMC-Na/saline water

      Solubility: 20 mg/mL (38.64 mM); Suspended solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (4.83 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (4.83 mM); Clear solution

    *All of the co-solvents are available by MedChemExpress (MCE).
    Purity & Documentation
    References
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    ML364 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    × = ×
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    Product Name:
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