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  4. PAβN dihydrochloride

PAβN dihydrochloride  (Synonyms: MC-207,110 dihydrochloride; Phe-Arg-β-naphthylamide dihydrochloride)

Cat. No.: HY-101444A Purity: 99.85%
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PAβN dihydrochloride (MC-207110 dihydrochloride) is an efflux pump inhibitor.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

CAS No. : 100929-99-5

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10 mM * 1 mL in DMSO
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Customer Review

Based on 11 publication(s) in Google Scholar

Other Forms of PAβN dihydrochloride:

PAβN dihydrochloride Related Antibodies

Top Publications Citing Use of Products

    PAβN dihydrochloride purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 Jul 22;44(7):115987.  [Abstract]

    Effect of efflux pump inhibitors (CCCP (10 mg/L), NMP (25 mg/L ), and PAβN dihydrochloride (25 mg/L)) on the susceptibility of evolved strains to tigecycline (n = 2). PAβN dihydrochloride lowered the MIC values and reversed the tigecycline-resistance phenotypes, suggesting a role for increased efflux pump activity in tigecycline resistance.

    PAβN dihydrochloride purchased from MedChemExpress. Usage Cited in: Front Microbiol. 2024 Sep 24:15:1417237.  [Abstract]

    The results of tigecycline combined with PAβN dihydrochloride (20 mg/L) demonstrated that 20 TNSKP strains exhibited a fold decrease ≥ 4 in MIC after co-incubation with PAβN, but 2 strains of TSKP did not show any change in MIC under the effect of efflux pump inhibitor.

    PAβN dihydrochloride purchased from MedChemExpress. Usage Cited in: Microb Pathog. 2023 Dec:185:106397.  [Abstract]

    PAβN dihydrochloride (50 μg/mL; 50 μL; 24 h)。 Results of screening test strains.

    PAβN dihydrochloride purchased from MedChemExpress. Usage Cited in: Front Cell Infect Microbiol. 2021 Mar 25;11:660431.  [Abstract]

    BBH uptake with pump inhibitors CCCP, PAβN dihydrochloride (50 µM; 60 min), and reserpine in ATCC 19606 strain, MDR-B and △adeB MDR-B strains, and MDR-TJ and △adeB MDR-TJ strains. Red arrows indicate the pump inhibitors (50 µM) were added at 60 min. Grey letters indicate the inhibitors were added to the adeB knockout strains.

    PAβN dihydrochloride purchased from MedChemExpress. Usage Cited in: Microb Drug Resist. 2020 Jun;26(6):550-560.  [Abstract]

    PAβN dihydrochloride (50-200 μg/mL) and NMP inhibited OqxB activity in a concentration-dependent manner, suggesting that an efflux pump (OqxAB) is indeed responsible for the decreased susceptibility of the overexpression system.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    PAβN dihydrochloride (MC-207110 dihydrochloride) is an efflux pump inhibitor.

    In Vitro

    PAβN increases the susceptibilities of the three pump-overexpressing mutants of P. aeruginosa to levofloxacin 64-fold. PAβN also potentiates the activity of levofloxacin against strain PAM2391 containing plasmid pAGH97 with the mexXY genes and against wild-type strain PAM1020. PAβN has an effect on susceptibilities to other antibiotics that are substrates of efflux pumps. PAβN increases levels of accumulation of efflux pump substrates inside the cell. It enhances the activity of levofloxacin against clinical isolates of P. aeruginosa[1]. PAβN reduces the MICs in nine ciprofloxacin-resistant isolates, and in four of these, PAβN increases the susceptibility by twofold. Moreover, PAβN restores ciprofloxacin susceptibility in five of the ciprofloxacin-resistant isolates. In addition, clear effects of NMP on the ciprofloxacin MICs are seen for 20 of these ciprofloxacin-resistant isolates[2]. PAβN permeabilizes bacterial membranes in a concentration-dependent manner at levels below those typically used in combination studies, and this additional mode of action should be considered when using PAβN as a control for efflux studies[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    PAβN dihydrochloride Related Antibodies
    Molecular Weight

    519.47

    Formula

    C25H32Cl2N6O2
    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 83.33 mg/mL (160.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 14.29 mg/mL (27.51 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9250 mL 9.6252 mL 19.2504 mL
    5 mM 0.3850 mL 1.9250 mL 3.8501 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (4.00 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (4.00 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.85%

    SDS (644 KB)

    COA (250 KB) LCMS (97 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 1.9250 mL 9.6252 mL 19.2504 mL 48.1260 mL
    5 mM 0.3850 mL 1.9250 mL 3.8501 mL 9.6252 mL
    10 mM 0.1925 mL 0.9625 mL 1.9250 mL 4.8126 mL
    15 mM 0.1283 mL 0.6417 mL 1.2834 mL 3.2084 mL
    20 mM 0.0963 mL 0.4813 mL 0.9625 mL 2.4063 mL
    25 mM 0.0770 mL 0.3850 mL 0.7700 mL 1.9250 mL
    DMSO 30 mM 0.0642 mL 0.3208 mL 0.6417 mL 1.6042 mL
    40 mM 0.0481 mL 0.2406 mL 0.4813 mL 1.2031 mL
    50 mM 0.0385 mL 0.1925 mL 0.3850 mL 0.9625 mL
    60 mM 0.0321 mL 0.1604 mL 0.3208 mL 0.8021 mL
    80 mM 0.0241 mL 0.1203 mL 0.2406 mL 0.6016 mL
    100 mM 0.0193 mL 0.0963 mL 0.1925 mL 0.4813 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    PAβN dihydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    PAβN100929-99-5MC-207,110Phe-Arg-β-naphthylamideBacterialInhibitorinhibitorinhibit

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