Art. -Nr.: HY-101444A

PAβN dihydrochloride

Name: PAβN dihydrochloride
Brand: MedChemExpress
SKU: HY-101444A
Price: 45.00 USD
Availability: InStock
Rating: 5 (12 reviews)

(Synonyms: MC-207,110 dihydrochloride; Phe-Arg-β-naphthylamide dihydrochloride)

12 Cited Publications

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Based on 12 publication(s) in Google Scholar

PAβN dihydrochloride (MC-207110 dihydrochloride) is an efflux pump inhibitor.

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Reinheit : 99.85%
CAS. Nr.: 100929-99-5
Formel: C₂₅H₃₂Cl₂N₆O₂
Molecular Weight:519.47

Speicherung:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

PAβN dihydrochloride Chemical Structure

CAS. Nr.: 100929-99-5

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Biologische Aktivität
- Beschreibung
- In Vitro
Chemical Information
Publications (12)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

PAβN dihydrochloride (MC-207110 dihydrochloride) is an efflux pump inhibitor.

In Vitro

PAβN increases the susceptibilities of the three pump-overexpressing mutants of P. aeruginosa to levofloxacin 64-fold. PAβN also potentiates the activity of levofloxacin against strain PAM2391 containing plasmid pAGH97 with the mexXY genes and against wild-type strain PAM1020. PAβN has an effect on susceptibilities to other antibiotics that are substrates of efflux pumps. PAβN increases levels of accumulation of efflux pump substrates inside the cell. It enhances the activity of levofloxacin against clinical isolates of P. aeruginosa^[1]. PAβN reduces the MICs in nine ciprofloxacin-resistant isolates, and in four of these, PAβN increases the susceptibility by twofold. Moreover, PAβN restores ciprofloxacin susceptibility in five of the ciprofloxacin-resistant isolates. In addition, clear effects of NMP on the ciprofloxacin MICs are seen for 20 of these ciprofloxacin-resistant isolates^[2]. PAβN permeabilizes bacterial membranes in a concentration-dependent manner at levels below those typically used in combination studies, and this additional mode of action should be considered when using PAβN as a control for efflux studies^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 100929-99-5
Appearance Solid
Molecular Weight 519.47
Formel C₂₅H₃₂Cl₂N₆O₂
Color White to light yellow
Synonyms

MC-207,110 dihydrochloride; Phe-Arg-β-naphthylamide dihydrochloride
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (12)

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Journal Impact Factor

Cell Rep

Lon dysfunction-mediated collateral sensitivity drives effective antibiotic combination therapies against tigecycline-resistant pathogens. [Abstract]

2025 Jul 22;44(7):115987. PMID: 40650911

PAβN dihydrochloride purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 Jul 22;44(7):115987. [Abstract]

Effect of efflux pump inhibitors (CCCP (10 mg/L), NMP (25 mg/L ), and PAβN dihydrochloride (25 mg/L)) on the susceptibility of evolved strains to tigecycline (n = 2). PAβN dihydrochloride lowered the MIC values and reversed the tigecycline-resistance phenotypes, suggesting a role for increased efflux pump activity in tigecycline resistance.
Front Microbiol

Comparison of antimicrobial activities and resistance mechanisms of eravacycline and tigecycline against clinical Acinetobacter baumannii isolates in China. [Abstract]

2024 Sep 24:15:1417237. PMID: 39380684

PAβN dihydrochloride purchased from MedChemExpress. Usage Cited in: Front Microbiol. 2024 Sep 24:15:1417237. [Abstract]

The results of tigecycline combined with PAβN dihydrochloride (20 mg/L) demonstrated that 20 TNSKP strains exhibited a fold decrease ≥ 4 in MIC after co-incubation with PAβN, but 2 strains of TSKP did not show any change in MIC under the effect of efflux pump inhibitor.
Front Cell Infect Microbiol

A Potential Combination Therapy of Berberine Hydrochloride With Antibiotics Against Multidrug-Resistant Acinetobacter baumannii. [Abstract]

2021 Mar 25:11:660431. PMID: 33842399

PAβN dihydrochloride purchased from MedChemExpress. Usage Cited in: Front Cell Infect Microbiol. 2021 Mar 25:11:660431. [Abstract]

BBH uptake with pump inhibitors CCCP, PAβN dihydrochloride (50 µM; 60 min), and reserpine in ATCC 19606 strain, MDR-B and △adeB MDR-B strains, and MDR-TJ and △adeB MDR-TJ strains. Red arrows indicate the pump inhibitors (50 µM) were added at 60 min. Grey letters indicate the inhibitors were added to the adeB knockout strains.
World J Microbiol Biotechnol

AcrAB-TolC efflux pump overexpression and tet(A) gene mutation increase tigecycline resistance in Klebsiella pneumoniae. [Abstract]

2024 Jun 6;40(8):233. PMID: 38842631
Microbiol Spectr

OmpK36 deficiency and inducible AmpC β-lactamase synergistically drive imipenem resistance in Klebsiella aerogenes. [Abstract]

2026 Jul 7;14(7):e0410825. PMID: 42212662
Microbiol Spectr

IITR00803: a benzoxazole-nitrothiophene small molecule with broad-spectrum antibacterial potential. [Abstract]

2025 Sep 2;13(9):e0114425. PMID: 40792514
J Antimicrob Chemother

Identification of a novel plasmid-mediated tigecycline resistance-related gene, tet(Y), in Acinetobacter baumannii. [Abstract]

2021 Dec 24;77(1):58-68. PMID: 34634801
mSphere

RND Pump-Mediated Efflux of Amotosalen, a Compound Used in Pathogen Inactivation Technology to Enhance Safety of Blood Transfusion Products, May Compromise Its Gram-Negative Anti-Bacterial Activity. [Abstract]

2023 Apr 20;8(2):e0067322. PMID: 36853056
Microb Pathog

Effect of piperine on the inhibitory potential of MexAB-OprM efflux pump and imipenem resistance in carbapenem-resistant Pseudomonas aeruginosa. [Abstract]

2023 Dec:185:106397. PMID: 37852553

PAβN dihydrochloride purchased from MedChemExpress. Usage Cited in: Microb Pathog. 2023 Dec:185:106397. [Abstract]

PAβN dihydrochloride (50 μg/mL; 50 μL; 24 h)。 Results of screening test strains.
Microb Drug Resist

Site-Directed Mutagenesis Reveals Crucial Residues in Escherichia coli Resistance-Nodulation-Division Efflux Pump OqxB. [Abstract]

2020 Jun;26(6):550-560. PMID: 31834851

PAβN dihydrochloride purchased from MedChemExpress. Usage Cited in: Microb Drug Resist. 2020 Jun;26(6):550-560. [Abstract]

PAβN dihydrochloride (50-200 μg/mL) and NMP inhibited OqxB activity in a concentration-dependent manner, suggesting that an efflux pump (OqxAB) is indeed responsible for the decreased susceptibility of the overexpression system.
Al-Rafidain Journal of Medical Sciences

2025 Feb 11.
bioRxiv

2020 Apr.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 83.33 mg/mL (160.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 14.29 mg/mL (27.51 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.9250 mL

9.6252 mL

19.2504 mL

5 mM

0.3850 mL

1.9250 mL

3.8501 mL

10 mM

0.1925 mL

0.9625 mL

1.9250 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (4.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (4.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (644 KB)
- English - EN (644 KB)
- Français - FR (644 KB)
- Deutsch - DE (644 KB)
- Norwegian - NO (644 KB)
- Español - ES (644 KB)
- Swedish - SV (644 KB)
- Italian - IT (644 KB)
- Korean - KR (644 KB)
- Portuguese - PT (644 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Lomovskaya O, et al. Identification and characterization of inhibitors of multidrug resistance efflux pumps in Pseudomonas aeruginosa: novel agents for combination therapy. Antimicrob Agents Chemother. 2001 Jan;45(1):105-16. [Content Brief]

[2]. Kurin?i? M, et al. Effects of efflux pump inhibitors on erythromycin, ciprofloxacin, and tetracycline resistance in Campylobacter spp. isolates. Microb Drug Resist. 2012 Oct;18(5):492-501. [Content Brief]

[3]. Lamers RP, et al. The efflux inhibitor phenylalanine-arginine beta-naphthylamide (PAβN) permeabilizes the outer membrane of gram-negative bacteria. PLoS One. 2013;8(3):e60666. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	1.9250 mL	9.6252 mL	19.2504 mL	48.1260 mL
	5 mM	0.3850 mL	1.9250 mL	3.8501 mL	9.6252 mL
	10 mM	0.1925 mL	0.9625 mL	1.9250 mL	4.8126 mL
	15 mM	0.1283 mL	0.6417 mL	1.2834 mL	3.2084 mL
	20 mM	0.0963 mL	0.4813 mL	0.9625 mL	2.4063 mL
	25 mM	0.0770 mL	0.3850 mL	0.7700 mL	1.9250 mL
DMSO	30 mM	0.0642 mL	0.3208 mL	0.6417 mL	1.6042 mL
	40 mM	0.0481 mL	0.2406 mL	0.4813 mL	1.2031 mL
	50 mM	0.0385 mL	0.1925 mL	0.3850 mL	0.9625 mL
	60 mM	0.0321 mL	0.1604 mL	0.3208 mL	0.8021 mL
	80 mM	0.0241 mL	0.1203 mL	0.2406 mL	0.6016 mL
	100 mM	0.0193 mL	0.0963 mL	0.1925 mL	0.4813 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Bioz

Keywords

PAβN dihydrochloride

12 Cited Publications

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PAβN dihydrochloride

Publications Citing Use of MedChemExpress (MCE) PAβN dihydrochloride