1. Metabolic Enzyme/Protease
  2. E1/E2/E3 Enzyme

PRT4165 (Synonyms: NSC600157)

Cat. No.: HY-19817 Purity: >98.0%
Handling Instructions

PRT4165 is a potent inhibitor of PRC1 (Polycomb-repressive complex 1)-mediated H2A ubiquitylation.

For research use only. We do not sell to patients.
PRT4165 Chemical Structure

PRT4165 Chemical Structure

CAS No. : 31083-55-3

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 66 In-stock
5 mg USD 60 In-stock
10 mg USD 84 In-stock
50 mg USD 264 In-stock
100 mg USD 480 In-stock
200 mg   Get quote  
500 mg   Get quote  

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

PRT4165 is a potent inhibitor of PRC1 (Polycomb-repressive complex 1)-mediated H2A ubiquitylation. Target: PRC1 in vitro: PRT4165 is a novel chromatin-remodeling compound and provide a new tool for the inhibition of ubiquitylation signaling at DNA double-strand breaks.PRT4165 inhibits histone H2A E3 ubiquitin ligase activity of RING1. PRT4165 Inhibits the E3 Ubiquitin Ligase Activity of PRC1. PRT4165 efficiently inhibits H2A ubiquitylation at lysine 119 (uH2AK119) and that this inhibition is dependent on both the concentration of PRT4165 and the incubation time with PRT4165. Treatment of cells for 60 min with 50 μm PRT4165 results in a dramatic reduction in total ubiquitylated histone H2A. PRT4165 inhibits the accumulation of all detectable ubiquitin at sites of DNA double-strand breaks (DSBs), the retention of several DNA damage response proteins in foci that form around DSBs, and the repair of the DSBs. [1] in vivo: PRT4165 inhibits the PRC1 E3 monoubiquitin ligase activity toward γ-H2AX.[1]

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 4.2510 mL 21.2549 mL 42.5098 mL
5 mM 0.8502 mL 4.2510 mL 8.5020 mL
10 mM 0.4251 mL 2.1255 mL 4.2510 mL
Please refer to the solubility information to select the appropriate solvent.
References
Molecular Weight

235.24

Formula

C₁₅H₉NO₂

CAS No.

31083-55-3

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

10 mM in DMSO

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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PRT4165
Cat. No.:
HY-19817
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