1. Peptides

Prolylleucine (Synonyms: ((Benzyloxy)carbonyl)-L-prolyl-D-leucine)

Cat. No.: HY-112173 Purity: >98.0%
Handling Instructions

Prolylleucine is a dipeptide containing branched-chain amino acids. Sequence: Z-Pro-{d-Leu};ZP-{d-Leu}.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Prolylleucine Chemical Structure

Prolylleucine Chemical Structure

CAS No. : 61596-47-2

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
10 mg USD 50 In-stock
Estimated Time of Arrival: December 31
25 mg USD 90 In-stock
Estimated Time of Arrival: December 31
50 mg USD 140 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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  • Biological Activity

  • Technical Information

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  • References

Description

Prolylleucine is a dipeptide containing branched-chain amino acids. Sequence: Z-Pro-{d-Leu};ZP-{d-Leu}.

In Vitro

The addition of specific dipeptide containing branched-chain amino acids, such as Prolylleucine, to the growth medium negatively affects cell envelope-associated proteinase (CEP) activity, whereas dipeptides without branched-chain amino acids had no effect on the enzyme's production. To determine if certain peptides are involved in the regulation of CEP biosynthesis, eight specific dipeptides, one tripeptide, and two peptide fractions (LMMP and HMMP) from Casitone are evaluated. The CEP activity levels of L. delbrueckii subsp. lactis CRL 581 grown in MDM supplemented with LMMP are similar to those obtained in cells grown in minimal defined medium (MDM) supplemented with Casitone (99-fold reduction). The addition of leucylglycylglycine (LGG), leucylleucine (LL), leucylproline (LP), or Prolylleucine (PL) (final concentration, 1 mM) to MDM leads to a 6.5-, 7-, 4-, or 3.5-fold reduction in CEP activity, respectively. An increase of up to 5 mM in the concentration of these dipeptides results in a further two- or threefold reduction of CEP activity compared to the activity obtained in the presence of 1 mM of the peptide mentioned above. LGG, LL, LP, and Prolylleucine contain leucine as a branched-chain amino acid (BCAA). In contrast, no effect on CEP activity is observed by the supplementation of MDM with 1 to 5 mM of GT, PA, TG, GM, GP (dipeptides without BCAA), or HMMP. No inhibitory effect on proteinase activity from the presence of high concentrations (10-fold increase) of each of the 20 amino acids in the growth medium is observed. However, a 50-fold increase in BCAA concentration in MDM leads to a repression of proteinase synthesis of 40%. L. delbrueckii subsp. lactis is auxotrophic for BCAA[1].

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (275.92 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7592 mL 13.7961 mL 27.5923 mL
5 mM 0.5518 mL 2.7592 mL 5.5185 mL
10 mM 0.2759 mL 1.3796 mL 2.7592 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (6.90 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (6.90 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (6.90 mM); Clear solution

References
Molecular Weight

362.42

Formula

C₁₉H₂₆N₂O₅

CAS No.

61596-47-2

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
Prolylleucine
Cat. No.:
HY-112173
Quantity:

Prolylleucine

Cat. No.: HY-112173