Orexin A (human, rat, mouse) (Synonyms: Hypocretin-1 (human, rat, mouse))

Cat. No.: HY-106224: FAQs
Data Sheet SDS COA Handling Instructions

Orexin A (human, rat, mouse) (Hypocretin-1 (human, rat, mouse)), a 33 amino acid excitatory neuropeptide, orchestrates diverse central and peripheral processes. Orexin A (human, rat, mouse) is a specific, high-affinity agonist for G-protein-coupled receptor OX1R. Orexin A (human, rat, mouse) has a role in the regulation of feeding behavior. Orexin A (human, rat, mouse) is an effective anti-nociceptive and anti-hyperalgesic agent in mice and rats.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Orexin A (human, rat, mouse) Chemical Structure

CAS No. : 205640-90-0

Size	Price	Stock
1 mg	USD 400	Ask For Quote & Lead Time
5 mg	USD 1600	Ask For Quote & Lead Time

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Other In-stock Forms of Orexin A (human, rat, mouse):

Orexin A (human, rat, mouse) (TFA) Orexin A (human, rat, mouse) (acetate)

Other Forms of Orexin A (human, rat, mouse):

Orexin A (human, rat, mouse) (TFA) In-stock
Orexin A (human, rat, mouse) (acetate) In-stock

3 Publications Citing Use of MCE Orexin A (human, rat, mouse)

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

OX₁ Receptor

In Vitro

Orexin A (human, rat, mouse) has high affinity for OX1R, with 38 nM IC₅₀ and 34 nM EC₅₀ values in the the [Ca^2﹢]i transient assay^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Orexin A (human, rat, mouse) (3-30 mg/kg; i.v.; 5 min pre-test) significantly increases the latency to response at 10 and 30 mg/kg i.v. when given 5 min pre-test from 24.8±2.0 s in vehicle-treated mice to 35.0±3.7 s and 45.7±4.5 s, respectively^[2].
Orexin A (human, rat, mouse) (3, 10 and 30 mg/kg; i.v.) was given immediately before phenylp-quinone (PPQ) and increases the latency to the first PPQ-induced constriction from 357.4±35.2 s in vehicle-treated mice to 500.3±31.2 s at 10 mg/kg and 594.5±5.5 s at 30 mg/kg^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female mice (mouse carrageenan-induced thermal hyperalgesia test)^[2]
Dosage:	3, 10 and 30 mg/kg
Administration:	i.v.; 5 min pre-test
Result:	Significantly increased the latency to response at 10 and 30 mg/kg.

Molecular Weight

3561.10

Appearance

Solid

Formula

C₁₅₂H₂₄₃N₄₇O₄₄S₄

CAS No.

205640-90-0

Sequence Shortening

{Glp}-PLPDCCRQKTCSCRLYELLHGAGNHAAGILTL-NH2 (Disulfide bridge: Cys6-Cys12, Cys7-Cys14)

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O

Peptide Solubility and Storage Guidelines:

1. Calculate the length of the peptide.

2. Calculate the overall charge of the entire peptide according to the following table:

	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3. Recommended solution:

Overall charge of peptide	Details
Negative (<0)	1. Try to dissolve the peptide in water first. 2. If water fails, add NH₄OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)	1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

Purity & Documentation

Purity: 99.15%

Select Batch:

Data Sheet (264 KB) SDS (252 KB)

COA (242 KB) RP-HPLC (107 KB) MS (190 KB) Elemental Analysis Report (99 KB)

Handling Instructions (2659 KB)

References

[1]. Sakurai T, et al. Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior. Cell. 1998 Feb 20;92(4):573-85. [Content Brief]

[2]. Bingham S, et al. Orexin-A, an hypothalamic peptide with analgesic properties. Pain. 2001 May;92(1-2):81-90. [Content Brief]

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Keywords:

Orexin A (human, rat, mouse)205640-90-0Hypocretin-1 (human, rat, mouse)Orexin Receptor (OX Receptor)Hypocretin ReceptorHCRT ReceptoraffinityG-protein-coupledreceptorfeedingbehaviornociceptiveanti-hyperalgesicInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email address *

Phone number *

Organization name *

Department *

Requested quantity *

Country or Region *

Remarks