1. Peptides

Rat orexin A 

Cat. No.: HY-106224 Purity: 96.07%
Handling Instructions

Rat orexin A is an important neuropeptide involved in the regulation of feeding, arousal, energy consuming, and reward seeking in the body. Sequence: {Glp}-Pro-Leu-Pro-Asp-Cys-Cys-Arg-Gln-Lys-Thr-Cys-Ser-Cys-Arg-Leu-Tyr-Glu-Leu-Leu-His-Gly-Ala-Gly-Asn-His-Ala-Ala-Gly-IIE-Leu-Thr-Leu-NH2 (Disulfide bridge: Cys6-Cys12, Cys7-Cys14);{Glp}-PLPDCCRQKTCSCRLYELLHGAGNHAAGILTL-NH2 (Disulfide bridge: Cys6-Cys12, Cys7-Cys14).

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Rat orexin A Chemical Structure

Rat orexin A Chemical Structure

CAS No. : 205640-90-0

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Estimated Time of Arrival: December 31
5 mg USD 1600 In-stock
Estimated Time of Arrival: December 31
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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Rat orexin A is an important neuropeptide involved in the regulation of feeding, arousal, energy consuming, and reward seeking in the body. Sequence: {Glp}-Pro-Leu-Pro-Asp-Cys-Cys-Arg-Gln-Lys-Thr-Cys-Ser-Cys-Arg-Leu-Tyr-Glu-Leu-Leu-His-Gly-Ala-Gly-Asn-His-Ala-Ala-Gly-IIE-Leu-Thr-Leu-NH2 (Disulfide bridge: Cys6-Cys12, Cys7-Cys14);{Glp}-PLPDCCRQKTCSCRLYELLHGAGNHAAGILTL-NH2 (Disulfide bridge: Cys6-Cys12, Cys7-Cys14).

In Vitro

Rat orexin A is an important neuropeptide involved in the regulation of feeding, arousal, energy consuming, and reward seeking in the body. It is found that Rat orexin A promotes astrocytes migration in different time and concentrations. Rat orexin A has a maximal effect at 10 nM and 24 h treatment (increases the migrating distance of the astrocytes into ~240%, increases the migrating area of the astrocytes into ~190%). Results also demonstrate that Rat orexin A-induced phosphorylation of ERK1/2 is significantly elevated (0.38±0.03 in Rat orexin A 10 nM group vs. 0.21±0.01 in control group, n=3, p<0.05) in primary cortical astrocytes.

In Vivo

Rat orexin A delays the day of vaginal opening in rats of Group 4 comparing to that in Group 3. Results indicate that injection of Rat orexin A can ameliorate central precocious puberty in rats. Lower mRNA level of MEG3 is observed in precocious puberty rats injected with Rat orexin A than that in precocious puberty rats injected with normal saline. Rat orexin A also reverses the dysregulation of Kisspeptin in hypothalamus of central precocious puberty rats[2]. In A6, many or most dopamine β hydroxylase (DBH)-positive neurons appear to be activated by Rat orexin A. Results also demonstrate that food intake is significantly enhanced by local Rat orexin A injection (P<0.05; n=9)[3].

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
References
Cell Assay
[1]

Astrocyte cells are grown to confluence in dishes and starved with serum-free DMED/F12 medium for 24 h. The monolayer cells are manually scratched in the center of the dishes with a bright and clear field. The detached cells are removed by washing the cells once with PBS. Serum-free DMEM/F12 medium with or without Rat orexin A is added to each dish as indicated after pretreatment with the inhibitor for 30 min. Then the cells are separately fixed with 4% paraformaldehyde and labeled with anti-GFAP (1:200) for photography and analyzing of the cell migration at 0, 24 and 48 h. Images of migratory cells from the scratched boundary are observed and acquired at different time with a digital camera and a light microscope[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

Five-days-old specific pathogen free grade female SD rats, weighing (25±5) g are used in this study. After two days of adaptive feeding, rats are randomly divided into four groups: Group 1 (normal control, n=12), Group 2 (precocious puberty rats, n=12), Group 3 (precocious puberty rats treated with normal saline, n=12), Group 4 (precocious puberty rats treated with Rat orexin A, n=12). Each rat in experimental groups 2, 3 and 4 are subcutaneously injected with 300 μg of danazol to establish the precocious puberty model. Each rat in groups 4 is injected with Rat orexin A (0.5 nM) for 1 time/day via lateral ventricle at 15-days-old. Each rat in groups 3 is injected with equal volumes of normal saline. When rats are 20-days-old, vaginal openings are checked and vaginal opening time (VO) is recorded[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

3561.10

Formula

C₁₅₂H₂₄₃N₄₇O₄₄S₄

CAS No.

205640-90-0

Storage

polypeptide,stored under nitrogen

Shipping

Room temperature in continental US; may vary elsewhere

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× = ×
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Product Name:
Rat orexin A
Cat. No.:
HY-106224
Quantity:

Rat orexin A

Cat. No.: HY-106224