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Rat orexin A 

Cat. No.: HY-106224 Purity: 96.07%
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Rat orexin A is an important neuropeptide involved in the regulation of feeding, arousal, energy consuming, and reward seeking in the body.

For research use only. We do not sell to patients.

Rat orexin A Chemical Structure

Rat orexin A Chemical Structure

CAS No. : 205640-90-0

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Description

Rat orexin A is an important neuropeptide involved in the regulation of feeding, arousal, energy consuming, and reward seeking in the body.

In Vitro

Rat orexin A is an important neuropeptide involved in the regulation of feeding, arousal, energy consuming, and reward seeking in the body. It is found that Rat orexin A promotes astrocytes migration in different time and concentrations. Rat orexin A has a maximal effect at 10 nM and 24 h treatment (increases the migrating distance of the astrocytes into ~240%, increases the migrating area of the astrocytes into ~190%). Results also demonstrate that Rat orexin A-induced phosphorylation of ERK1/2 is significantly elevated (0.38±0.03 in Rat orexin A 10 nM group vs. 0.21±0.01 in control group, n=3, p<0.05) in primary cortical astrocytes.

In Vivo

Rat orexin A delays the day of vaginal opening in rats of Group 4 comparing to that in Group 3. Results indicate that injection of Rat orexin A can ameliorate central precocious puberty in rats. Lower mRNA level of MEG3 is observed in precocious puberty rats injected with Rat orexin A than that in precocious puberty rats injected with normal saline. Rat orexin A also reverses the dysregulation of Kisspeptin in hypothalamus of central precocious puberty rats[2]. In A6, many or most dopamine β hydroxylase (DBH)-positive neurons appear to be activated by Rat orexin A. Results also demonstrate that food intake is significantly enhanced by local Rat orexin A injection (P<0.05; n=9)[3].

References
Cell Assay
[1]

Astrocyte cells are grown to confluence in dishes and starved with serum-free DMED/F12 medium for 24 h. The monolayer cells are manually scratched in the center of the dishes with a bright and clear field. The detached cells are removed by washing the cells once with PBS. Serum-free DMEM/F12 medium with or without Rat orexin A is added to each dish as indicated after pretreatment with the inhibitor for 30 min. Then the cells are separately fixed with 4% paraformaldehyde and labeled with anti-GFAP (1:200) for photography and analyzing of the cell migration at 0, 24 and 48 h. Images of migratory cells from the scratched boundary are observed and acquired at different time with a digital camera and a light microscope[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

Five-days-old specific pathogen free grade female SD rats, weighing (25±5) g are used in this study. After two days of adaptive feeding, rats are randomly divided into four groups: Group 1 (normal control, n=12), Group 2 (precocious puberty rats, n=12), Group 3 (precocious puberty rats treated with normal saline, n=12), Group 4 (precocious puberty rats treated with Rat orexin A, n=12). Each rat in experimental groups 2, 3 and 4 are subcutaneously injected with 300 μg of danazol to establish the precocious puberty model. Each rat in groups 4 is injected with Rat orexin A (0.5 nM) for 1 time/day via lateral ventricle at 15-days-old. Each rat in groups 3 is injected with equal volumes of normal saline. When rats are 20-days-old, vaginal openings are checked and vaginal opening time (VO) is recorded[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

3561.10

Formula

C₁₅₂H₂₄₃N₄₇O₄₄S₄

CAS No.

205640-90-0

Sequence

{Glp}-Pro-Leu-Pro-Asp-Cys-Cys-Arg-Gln-Lys-Thr-Cys-Ser-Cys-Arg-Leu-Tyr-Glu-Leu-Leu-His-Gly-Ala-Gly-Asn-His-Ala-Ala-Gly-IIE-Leu-Thr-Leu-NH2 (Disulfide bridge: Cys6-Cys12, Cys7-Cys14)

Sequence Shortening

{Glp}-PLPDCCRQKTCSCRLYELLHGAGNHAAGILTL-NH2 (Disulfide bridge: Cys6-Cys12, Cys7-Cys14)

SMILES

[XPLPDCCRQKTCSCRLYELLHGAGNHAAGILTL cyclic (6-12),(7-14)-bis(disulfide)]

Storage

polypeptide,stored under nitrogen

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
Rat orexin A
Cat. No.:
HY-106224
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Rat orexin A

Cat. No.: HY-106224