Orexin A (human, rat, mouse) acetate
Based on 12 publication(s) in Google Scholar
Orexin A (Hypocretin-1) (human, rat, mouse) acetate is a hypothalamic neuropeptide with analgesic properties (crosses the blood-brain barrier). Orexin A (human, rat, mouse) acetate binds and activates two types of G protein-coupled receptors, the orexin-1 receptor (OX1R) and the orexin-2 receptor (OX2R). Orexin A (human, rat, mouse) acetate can be used in studies of appetite regulation, neurodegenerative diseases and modulation of injurious messaging.
For research use only. We do not sell to patients.
- Purity: 99.53%
- Formula: C152H243N47O44S4.xC2H4O2
- Molecular Weight:3561.10 (free base)
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Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Orexin A (human, rat, mouse) acetate
More- Research (Wash D C). 2024 May 31:7:0384. [Abstract]
- J Neuroinflammation. 2026 May 5;23(1):216. [Abstract]
- J Neuroinflammation. 2024 May 17;21(1):131. [Abstract]
- CNS Neurosci Ther. 2025 Sep;31(9):e70606. [Abstract]
- CNS Neurosci Ther. 2024 Nov;30(11):e70096. [Abstract]
- Food Funct. 2024 Aug 27;15(17):8661-8673. [Abstract]
- Exp Neurol. 2025 Nov:393:115378. [Abstract]
- J Inflamm Res. 2021 May 18:14:2007-2017. [Abstract]
- Brain Res Bull. 2021 Apr:169:81-93. [Abstract]
- Neuropeptides. 2025 Mar:110:102501. [Abstract]
- Med Sci Monit. 2019 Apr 19:25:2886-2895. [Abstract]
- bioRxiv. 2026 Jan 23.
Customer Validation & Images
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RT-PCR
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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WB
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Histological Imaging/Staining
All Orexin Receptor (OX Receptor) Isoforms
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Biological Activity
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OX1 Receptor |
OX2 Receptor |
Orexin A (human, rat, mouse) acetate (0.1, 1, 10, 100 nM; 24 h) increases the expression of BDNF in SH-SY5Y human dopaminergic neuroblastoma cells in a dose-dependent manner[1].
Orexin A (human, rat, mouse) acetate (1 nM; 1, 3, 6, 12, 24 h) increases the expression of BDNF, TH, and PI3K in a time-dependent manner in SH-SY5Y cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:SH-SY5Y cells
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Concentration:0.1, 1, 10, 100 nM
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Incubation Time:24 h
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Result:Increased the expression of PI3K and TH in a dose-dependent manner.
Significantly induced BDNF expression at the dose of 1 and 10 nM.
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Cell Line:SH-SY5Y cells
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Concentration:1 nM
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Incubation Time:1, 3, 6, 12, 24 h
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Result:Showed the expression of PI3K rapidly increased by 72.6 % as soon as 3 h, which maintained at high levels in the following 24 h.
Increased the protein level of BDNF and the increased rate was 44.8%, and the protein level of TH significantly increased at 12 h by 48.5%.
Orexin A (human, rat, mouse) acetate (10, 30 mg/kg; i.v.; single) shows to be analgesic in the carrageenan-induced thermal hyperalgesia model and PPQ-induced mouse abdominal constriction model[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male C57BL/6 mice (10-week-old; 22-26 g; MPTP-Induced Mouse Model of PD)[1].
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Dosage:300 ng/mouse
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Administration:Intracerebroventricular injection; single daily for 8 days
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Result:Reversed MPTP-induced motor impairments via OX1R and increased the protein expression of BDNF in nigral dopaminergic neurons.
Improved MPTP-induced impairments in spatial Learning and memory and protected dopaminergic neurons against MPTP-induced neurotoxicity.
Attenuated the reduction of dopaminergic neurons in the substantia nigra and restored the reduction of dopaminergic fibers in the striatum.
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Animal Model:Female ICR mice (17-28 g; carrageenan-induced thermal hyperalgesia model)[2].
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Dosage:10, 30 mg/kg
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Administration:Intravenous injection; single; 5 min pre-test
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Result:Increased paw withdrawal latency to the thermal stimulus back to baseline levels.
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Animal Model:Male ICR mice (17-20 g; phenyl-p-quinone (PPQ)-induced mouse abdominal constriction model)[2].
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Dosage:3, 10, 30 mg/kg
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Administration:Intravenous injection; single; given immediately before PPQ
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Result:Increased the latency to the first PPQ-induced constriction from 357.4 s (blank control group) to 500.3 s at 10 mg/kg and 594.5 s at 30 mg/kg.
Chemical Information
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Appearance Solid
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Molecular Weight 3561.10 (free base)
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Formula C152H243N47O44S4.xC2H4O2
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Color White to off-white
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Synonyms
Hypocretin-1 (human, rat, mouse) acetate
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Sequence
{Glp}-Pro-Leu-Pro-Asp-Cys-Cys-Arg-Gln-Lys-Thr-Cys-Ser-Cys-Arg-Leu-Tyr-Glu-Leu-Leu-His-Gly-Ala-Gly-Asn-His-Ala-Ala-Gly-Ile-Leu-Thr-Leu-NH2 (Disulfide bridge: Cys6-Cys12, Cys7-Cys14)
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Sequence Shortening
{Glp}-PLPDCCRQKTCSCRLYELLHGAGNHAAGILTL-NH2 (Disulfide bridge: Cys6-Cys12, Cys7-Cys14)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Publications (12)
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Journal Impact Factor
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Most Recent
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Research (Wash D C)
Repetitive Transcranial Magnetic Stimulation (rTMS) Improves Cognitive Impairment and Intestinal Microecological Dysfunction Induced by High-Fat Diet in Rats. [Abstract]2024 May 31:7:0384. PMID: 38826566
Orexin A (human, rat, mouse) acetate purchased from MedChemExpress. Usage Cited in: Research (Wash D C). 2024 May 31:7:0384. [Abstract]
Schematic illustration of the effect of different concentrations of Orexin A (human, rat, mouse) on inflammatory factors expression in BV2. In addition, the mRNA expression of TNF-α, IL-6, IL-1β, and inducible nitric oxide synthase (iNOS) in different groups.
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J Neuroinflammation
SARS-CoV-2 infection is associated with hypothalamic orexin suppression and persistent cortical NeuN attenuation. [Abstract]2026 May 5;23(1):216. PMID: 42087199 -
J Neuroinflammation
2024 May 17;21(1):131. PMID: 38760784
Orexin A (human, rat, mouse) acetate purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2024 May 17;21(1):131. [Abstract]
Treatment with 250 µg/kg Orexin A (human, rat, mouse) increases 7-day survival. Orexin A (human, rat, mouse) was administered at 250 µg/kg, 30 min after CLP induction, in a volume of 2.5 µl per nostril (5.0 µl per mouse).
Orexin A (human, rat, mouse) acetate purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2024 May 17;21(1):131. [Abstract]
Treatment with 250 µg/kg Orexin A (human, rat, mouse) ameliorates cognitive and affective deficits in CLP-induced sepsis. Time in the central zone and distance of activity in the open field. Orexin A (human, rat, mouse) was administered at 250 µg/kg, 30 min after CLP induction, in a volume of 2.5 µl per nostril (5.0 µl per mouse).
Orexin A (human, rat, mouse) acetate purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2024 May 17;21(1):131. [Abstract]
Orexin A (human, rat, mouse) reduces inflammation by decreasing Rras expression and impeding activation of the RAS/MAPK pathway.
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CNS Neurosci Ther
Neuroprotective Effects of Transcranial Pulsed Current Stimulation: Modulation of Microglial Polarization in Traumatic Brain Injury. [Abstract]2025 Sep;31(9):e70606. PMID: 40916543 -
CNS Neurosci Ther
Orexin-A Attenuates the Inflammatory Response in Sepsis-Associated Encephalopathy by Modulating Oxidative Stress and Inhibiting the ERK/NF-κB Signaling Pathway in Microglia and Astrocytes. [Abstract]2024 Nov;30(11):e70096. PMID: 39508266
Orexin A (human, rat, mouse) acetate purchased from MedChemExpress. Usage Cited in: CNS Neurosci Ther. 2024 Nov;30(11):e70096. [Abstract]
Orexin A (human, rat, mouse) treatment attenuates hippocampal neuronal damage after CLP. Intranasal administration of 250 μg/kg of Orexin A (human, rat, mouse) drug, 2.5 μL per nostril, and 5.0 μL per mouse was used in this experiment. TUNEL staining showing neuronal apoptosis and quantification of TUNEL (green) with DAPI (blue) in the CA1 area of the hippocampus 7 days after CLP.
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Food Funct
A high-fat diet induced depression-like phenotype via hypocretin-HCRTR1 mediated inflammation activation. [Abstract]2024 Aug 27;15(17):8661-8673. PMID: 39056112 -
Exp Neurol
Orexin A alleviates chronic cerebral hypoperfusion-induced neuroinflammation and cognitive dysfunction by inhibiting the NEK7/NLRP3 pathway. [Abstract]2025 Nov:393:115378. PMID: 40691984 -
J Inflamm Res
Orexin-A Attenuates Inflammatory Responses in Lipopolysaccharide-Induced Neural Stem Cells by Regulating NF-KB and Phosphorylation of MAPK/P38/Erk Pathways. [Abstract]2021 May 18:14:2007-2017. PMID: 34040413 -
Brain Res Bull
Trigeminal nerve electrical stimulation: An effective arousal treatment for loss of consciousness. [Abstract]2021 Apr:169:81-93. PMID: 33453332 -
Neuropeptides
Transcranial pulsed current stimulation alleviates neuronal pyroptosis and neurological dysfunction following traumatic brain injury via the orexin-A/NLRP3 pathway. [Abstract]2025 Mar:110:102501. PMID: 39764896 -
Med Sci Monit
2019 Apr 19:25:2886-2895. PMID: 31002658 -
Solvent & Solubility
H2O : ≥ 50 mg/mL
* "≥" means soluble, but saturation unknown.
Purity & Documentation
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Data Sheet (290 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Liu MF, et al. Orexin-A Exerts Neuroprotective Effects via OX1R in Parkinson's Disease. Front Neurosci. 2018 Nov 15;12:835. [Content Brief]
[2]. Bingham S, et al. Orexin-A, an hypothalamic peptide with analgesic properties. Pain. 2001 May;92(1-2):81-90. [Content Brief]
[3]. Rodgers RJ, et al. Orexins and appetite regulation. Neuropeptides. 2002 Oct;36(5):303-25. [Content Brief]
[4]. Kaminski T, et al. Expression of orexin receptors in the pituitary. Vitam Horm. 2012;89:61-73. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Orexin A (human, rat, mouse) acetate Related Classifications
HY-106224B Related Classifications
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