1. PI3K/Akt/mTOR
  2. Akt
  3. SC79

SC79, a unique specific and BBB permeable Akt activator, activates Akt in the cytosol and inhibits Akt membrane translocation. SC79 specifically binds to the PH domain of Akt.

For research use only. We do not sell to patients.

SC79 Chemical Structure

SC79 Chemical Structure

CAS No. : 305834-79-1

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1 mg USD 30 In-stock
5 mg USD 66 In-stock
10 mg USD 110 In-stock
50 mg USD 250 In-stock
100 mg USD 400 In-stock
200 mg USD 640 In-stock
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Customer Review

Based on 165 publication(s) in Google Scholar

Top Publications Citing Use of Products

154 Publications Citing Use of MCE SC79

WB
IF
Cell Viability Assay

    SC79 purchased from MedChemExpress. Usage Cited in: Environ Pollut. 2023 Feb 17.

    SC79 induces mitochondria dysfunction and apoptosis in the heart of zebrafish embryos (Fig D and E).

    SC79 purchased from MedChemExpress. Usage Cited in: Biopolymers. 2023 Mar 28.

    SC79 (5.0, 7.5, 10 μM; 24 h) significantly induces cell death in T84 cells.

    SC79 purchased from MedChemExpress. Usage Cited in: Biopolymers. 2023 Mar 28.

    SC79 (2.5 μM; 4, 6, 24 h) significantly increases the expression of p-AKT and p-AKT/AKT while decreases p-ERK expression and the ratio of p-ERK/ERK ratio in T84 cells.

    SC79 purchased from MedChemExpress. Usage Cited in: Toxicology. 2019 May 1;419:32-39.  [Abstract]

    HaCaT cells are treated with SC79 at 10 μM for 0, 0.5, 1, and 2 h, following arecoline treatment of 48 h. Proteins are extracted and used for Western blotting for phosphorylated and total Akt, mTOR, 4E-BP1, and cyclin D1. Akt, mTOR, 4E-BP1 and GAPDH are used as the internal control for p-Akt, p-mTOR, p-4E-BP1 and cyclin D1, respectively.

    SC79 purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2019 May;19(5):4091-4100.  [Abstract]

    Western blot analysis demonstrating the protein levels of LC3, p62, AKT, p-AKT, mTOR and p-mTOR following treatment with Aβ1-42, 3-MA, SC79 and SC79 + RAPA for 48 h, and the expression of the AKT/mTOR signaling pathway‑associated proteins of AKT, p-AKT, mTOR and p-mTOR following treatment with Aβ1-42, 3-MA, SC79 and SC79 + RAPA for 48 h.

    SC79 purchased from MedChemExpress. Usage Cited in: Acta Biomater. 2018 Nov;81:278-292.  [Abstract]

    PC12 cells are pretreated with 740-YP (30 μM), or SC79 (13.7 μM), or 3BDO (100 μM) for 1 h, and for the duration of GO (50 μg/mL) incubation for 24 h. LC3 turnover is detected by western blotting.

    SC79 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2018 Oct;106:755-762.  [Abstract]

    LTEP-a-2 cells are exposed to SC79 and GSK3β and AKT phosphorylation levels are significantly increased.

    SC79 purchased from MedChemExpress. Usage Cited in: Nutrients. 2018 Sep 23;10(10). pii: E1366.  [Abstract]

    Competition tests of SC79, PHT-427, AT7867, and AKT inhibitor VIII with the CGA probe against enriched AKT by CGA-modified functionalized MMs. Bands of AKT are detected by Western blot.

    SC79 purchased from MedChemExpress. Usage Cited in: Oncol Rep. 2019 Feb;41(2):811-818.  [Abstract]

    Cells are cultured with and without CAR/SC79 for 24 h, and cell lysates are prepared and analyzed by western blotting to detect the levels of MDM2 and p53Ser15.

    SC79 purchased from MedChemExpress. Usage Cited in: Toxicology. 2017 Jul 1;386:72-83  [Abstract]

    Western blot assays and quantitative analysis show that BPDE treatment reduces the expression levels of AKT and eNOS; while simultaneous addition of SC79 significantly increases their expression.

    SC79 purchased from MedChemExpress. Usage Cited in: Royal Society of Chemistry. 11th August 2017

    Phillygenin (Phi) modulates phosphorylation of Akt in BEAS-2B cells. BEAS-2B cells are serum-starved overnight and then treated with SC79 and different doses of Phi for 4 h. Thr 308 and Ser 473 phosphorylation of Akt and total Akt are detected by western blot. β-Actin is used as an internal control.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    SC79, a unique specific and BBB permeable Akt activator, activates Akt in the cytosol and inhibits Akt membrane translocation. SC79 specifically binds to the PH domain of Akt[1][2][3].

    In Vitro

    SC79 augmentes Akt phosphorylation at both the Thr308 and S473 sites[1].
    ? SC79 (10.96 μM) induces cytosolic phosphorylation of Akt. SC79 enhances IGF1-induced Akt phosphorylation in both serum-starved cells and cells grown in serum-rich medium[1].
    ? SC79 reduces neuronal excitotoxicity and prevents stroke-induced neuronal death. SC79 suppresses PHAKTM-GFP plasma membrane translocation[1].
    ? SC79 restores proliferation of BRAT1 knockdown cells, and reduces the production of superoxide in mitochondria of MitoSox positive cells[2].
    ? SC79 upregulates FLIPL/S expression and consequently suppresses caspase-8 activation[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Western Blot Analysis[1]

    Cell Line: HeLa cells.
    Concentration: 4 μg/mL (10.96 μM).
    Incubation Time: 30 min.
    Result: Induced cytosolic phosphorylation of Akt.
    In Vivo

    SC79 treatment, even at much high dose (0.4 mg/g of body weight), does not induce any detectable changes in body weight, survival rate, appearance, and behavior in mice[1].
    ? SC79 (10 mg/kg, i.p.) protects C57BL/6 mice from fas-induced fulminant hepatic failure[4].
    ? SC79 protects hepatocytes from TNFα-mediated apoptosis and mice from Gal/LPS-induced liver injury and damage[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male, age-matched (6- to 8-weekeold) C57BL/6 or BALB/c mice weighing 16 to 18 g[4].
    Dosage: 10 mg/kg.
    Administration: Intraperitoneally at 0.5 hour before the i.p. administration of an agonistic anti-Fas Jo2 antibody at a lethal dose of 0.5 and 0.4 mg/kg for C57BL/6 and BALB/c mice, respectively.
    Result: Treatment of mice with 10 mg/kg of SC79 at 0.5 hour before Jo2 injection increased mouse survival at 12 hours after Jo2 injection from 0% to 35%, and no additional mortality was observed to the end of the 2-month observation period.
    Animal Model: Male, age-matched (6 to 8 weeks old) C57BL/6 mice weighing 16-18 g[5].
    Dosage: 10 mg/kg.
    Administration: Intraperitoneally at 0.5 h before i.p. administration of 400 mg/kg of D-galactosamine (D-Gal) and 60 µg/kg of lipopolysaccharide (LPS) for C57BL/6 mice.
    Result: Gal/LPS challenge there was more bleeding on the liver of the vehicle control-treated mice as compared to that of SC79-treated mice.
    A single dose of SC79 significantly reduced Gal/LPS-mediated liver damage but not an infiltration of inflammatory cells in liver sections.
    Molecular Weight

    364.78

    Formula

    C17H17ClN2O5

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(OCC)C(C#N)C1C(C(OCC)=O)=C(N)OC2=CC=C(Cl)C=C12

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years

    *The compound is unstable in solutions, freshly prepared is recommended.

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (274.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Ethanol : 50 mg/mL (137.07 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.7414 mL 13.7069 mL 27.4138 mL
    5 mM 0.5483 mL 2.7414 mL 5.4828 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 5 mg/mL (13.71 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% EtOH    90% Corn Oil

      Solubility: ≥ 5 mg/mL (13.71 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *The compound is unstable in solutions, freshly prepared is recommended.

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: ≥98.0%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Ethanol / DMSO 1 mM 2.7414 mL 13.7069 mL 27.4138 mL 68.5345 mL
    5 mM 0.5483 mL 2.7414 mL 5.4828 mL 13.7069 mL
    10 mM 0.2741 mL 1.3707 mL 2.7414 mL 6.8534 mL
    15 mM 0.1828 mL 0.9138 mL 1.8276 mL 4.5690 mL
    20 mM 0.1371 mL 0.6853 mL 1.3707 mL 3.4267 mL
    25 mM 0.1097 mL 0.5483 mL 1.0966 mL 2.7414 mL
    30 mM 0.0914 mL 0.4569 mL 0.9138 mL 2.2845 mL
    40 mM 0.0685 mL 0.3427 mL 0.6853 mL 1.7134 mL
    50 mM 0.0548 mL 0.2741 mL 0.5483 mL 1.3707 mL
    60 mM 0.0457 mL 0.2284 mL 0.4569 mL 1.1422 mL
    80 mM 0.0343 mL 0.1713 mL 0.3427 mL 0.8567 mL
    100 mM 0.0274 mL 0.1371 mL 0.2741 mL 0.6853 mL
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    SC79 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Cat. No.:
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