1. PI3K/Akt/mTOR
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  3. SC79

SC79 

Cat. No.: HY-18749 Purity: >98.0%
Handling Instructions

SC79, a unique specific and BBB permeable Akt activator, activates Akt in the cytosol and inhibits Akt membrane translocation. SC79 specifically binds to the PH domain of Akt.

For research use only. We do not sell to patients.

SC79 Chemical Structure

SC79 Chemical Structure

CAS No. : 305834-79-1

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Customer Review

Based on 44 publication(s) in Google Scholar

Top Publications Citing Use of Products

Publications Citing Use of MCE SC79

    SC79 purchased from MCE. Usage Cited in: Toxicology. 2017 Jul 1;386:72-83

    Western blot assays and quantitative analysis show that BPDE treatment reduces the expression levels of AKT and eNOS; while simultaneous addition of SC79 significantly increases their expression.

    SC79 purchased from MCE. Usage Cited in: Royal Society of Chemistry. 11th August 2017

    Phillygenin (Phi) modulates phosphorylation of Akt in BEAS-2B cells. BEAS-2B cells are serum-starved overnight and then treated with SC79 and different doses of Phi for 4 h. Thr 308 and Ser 473 phosphorylation of Akt and total Akt are detected by western blot. β-Actin is used as an internal control.

    SC79 purchased from MCE. Usage Cited in: Nutrients. 2018 Sep 23;10(10). pii: E1366.

    Competition tests of SC79, PHT-427, AT7867, and AKT inhibitor VIII with the CGA probe against enriched AKT by CGA-modified functionalized MMs. Bands of AKT are detected by Western blot.

    SC79 purchased from MCE. Usage Cited in: Acta Biomater. 2018 Nov;81:278-292.

    PC12 cells are pretreated with 740-YP (30 μM), or SC79 (13.7 μM), or 3BDO (100 μM) for 1 h, and for the duration of GO (50 μg/mL) incubation for 24 h. LC3 turnover is detected by western blotting.

    SC79 purchased from MCE. Usage Cited in: Biomed Pharmacother. 2018 Oct;106:755-762.

    LTEP-a-2 cells are exposed to SC79 and GSK3β and AKT phosphorylation levels are significantly increased.

    SC79 purchased from MCE. Usage Cited in: Oncol Rep. 2019 Feb;41(2):811-818.

    Cells are cultured with and without CAR/SC79 for 24 h, and cell lysates are prepared and analyzed by western blotting to detect the levels of MDM2 and p53Ser15.

    SC79 purchased from MCE. Usage Cited in: Toxicology. 2019 May 1;419:32-39.

    HaCaT cells are treated with SC79 at 10 μM for 0, 0.5, 1, and 2 h, following arecoline treatment of 48 h. Proteins are extracted and used for Western blotting for phosphorylated and total Akt, mTOR, 4E-BP1, and cyclin D1. Akt, mTOR, 4E-BP1 and GAPDH are used as the internal control for p-Akt, p-mTOR, p-4E-BP1 and cyclin D1, respectively.

    SC79 purchased from MCE. Usage Cited in: Mol Med Rep. 2019 May;19(5):4091-4100.

    Western blot analysis demonstrating the protein levels of LC3, p62, AKT, p-AKT, mTOR and p-mTOR following treatment with Aβ1-42, 3-MA, SC79 and SC79 + RAPA for 48 h, and the expression of the AKT/mTOR signaling pathway‑associated proteins of AKT, p-AKT, mTOR and p-mTOR following treatment with Aβ1-42, 3-MA, SC79 and SC79 + RAPA for 48 h.

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    Description

    SC79, a unique specific and BBB permeable Akt activator, activates Akt in the cytosol and inhibits Akt membrane translocation. SC79 specifically binds to the PH domain of Akt[1][2][3].

    IC50 & Target[1]

    Akt

     

    In Vitro

    SC79 augmentes Akt phosphorylation at both the Thr308 and S473 sites[1].
    SC79 (10.96 μM) induces cytosolic phosphorylation of Akt. SC79 enhances IGF1-induced Akt phosphorylation in both serum-starved cells and cells grown in serum-rich medium[1].
    SC79 reduces neuronal excitotoxicity and prevents stroke-induced neuronal death. SC79 suppresses PHAKTM-GFP plasma membrane translocation[1].
    SC79 restores proliferation of BRAT1 knockdown cells, and reduces the production of superoxide in mitochondria of MitoSox positive cells[2].
    SC79 upregulates FLIPL/S expression and consequently suppresses caspase-8 activation[5].

    Western Blot Analysis[1]

    Cell Line: HeLa cells.
    Concentration: 4 μg/mL (10.96 μM).
    Incubation Time: 30 min.
    Result: Induced cytosolic phosphorylation of Akt.
    In Vivo

    SC79 treatment, even at much high dose (0.4 mg/g of body weight), does not induce any detectable changes in body weight, survival rate, appearance, and behavior in mice[1].
    SC79 (10 mg/kg, i.p.) Protects C57BL/6 mice from fas-induced fulminant hepatic failure[4].
    SC79 protects hepatocytes from TNFα-mediated apoptosis and mice from Gal/LPS-induced liver injury and damage[5].

    Animal Model: Male, age-matched (6- to 8-weekeold) C57BL/6 or BALB/c mice weighing 16 to 18 g[4].
    Dosage: 10 mg/kg.
    Administration: Intraperitoneally at 0.5 hour before the i.p. administration of an agonistic anti-Fas Jo2 antibody at a lethal dose of 0.5 and 0.4 mg/kg for C57BL/6 and BALB/c mice, respectively.
    Result: Treatment of mice with 10 mg/kg of SC79 at 0.5 hour before Jo2 injection increased mouse survival at 12 hours after Jo2 injection from 0% to 35%, and no additional mortality was observed to the end of the 2-month observation period.
    Animal Model: Male, age-matched (6 to 8 weeks old) C57BL/6 mice weighing 16-18 g[5].
    Dosage: 10 mg/kg.
    Administration: Intraperitoneally at 0.5 h before i.p. administration of 400 mg/kg of D-galactosamine (D-Gal) and 60 µg/kg of lipopolysaccharide (LPS) for C57BL/6 mice.
    Result: Gal/LPS challenge there was more bleeding on the liver of the vehicle control-treated mice as compared to that of SC79-treated mice.
    A single dose of SC79 significantly reduced Gal/LPS-mediated liver damage but not an infiltration of inflammatory cells in liver sections.
    Molecular Weight

    364.78

    Formula

    C₁₇H₁₇ClN₂O₅

    CAS No.

    305834-79-1

    SMILES

    O=C(OCC)C(C#N)C1C(C(OCC)=O)=C(N)OC2=CC=C(Cl)C=C12

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
      4°C 2 years

    *The compound is unstable in solutions, freshly prepared is recommended.

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (274.14 mM)

    H2O : < 0.1 mg/mL (insoluble)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.7414 mL 13.7069 mL 27.4138 mL
    5 mM 0.5483 mL 2.7414 mL 5.4828 mL
    10 mM 0.2741 mL 1.3707 mL 2.7414 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (6.85 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (6.85 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References

    Purity: >98.0%

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    Keywords:

    SC79SC 79SC-79AktPKBProtein kinase BInhibitorinhibitorinhibit

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