1. PI3K/Akt/mTOR
  2. Akt

SC79 

Cat. No.: HY-18749 Purity: >98.0%
Handling Instructions

SC79 is a selective and cell-permeable Akt activator which activates Akt phosphorylation and inhibits Akt membrane translocation.

For research use only. We do not sell to patients.

SC79 Chemical Structure

SC79 Chemical Structure

CAS No. : 305834-79-1

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
5 mg USD 60 In-stock
Estimated Time of Arrival: December 31
10 mg USD 100 In-stock
Estimated Time of Arrival: December 31
50 mg USD 300 In-stock
Estimated Time of Arrival: December 31
100 mg USD 420 In-stock
Estimated Time of Arrival: December 31
200 mg USD 650 In-stock
Estimated Time of Arrival: December 31
500 mg   Get quote  
1 g   Get quote  

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Top Publications Citing Use of Products

    SC79 purchased from MCE. Usage Cited in: Toxicology. 2017 Jul 1;386:72-83

    Western blot assays and quantitative analysis show that BPDE treatment reduces the expression levels of AKT and eNOS; while simultaneous addition of SC79 significantly increases their expression.

    SC79 purchased from MCE. Usage Cited in: Royal Society of Chemistry. 11th August 2017

    Phillygenin (Phi) modulates phosphorylation of Akt in BEAS-2B cells. BEAS-2B cells are serum-starved overnight and then treated with SC79 and different doses of Phi for 4 h. Thr 308 and Ser 473 phosphorylation of Akt and total Akt are detected by western blot. β-Actin is used as an internal control.

    SC79 purchased from MCE. Usage Cited in: Nutrients. 2018 Sep 23;10(10). pii: E1366.

    Competition tests of SC79, PHT-427, AT7867, and AKT inhibitor VIII with the CGA probe against enriched AKT by CGA-modified functionalized MMs. Bands of AKT are detected by Western blot.

    SC79 purchased from MCE. Usage Cited in: Acta Biomater. 2018 Nov;81:278-292.

    PC12 cells are pretreated with 740-YP (30 μM), or SC79 (13.7 μM), or 3BDO (100 μM) for 1 h, and for the duration of GO (50 μg/mL) incubation for 24 h. LC3 turnover is detected by western blotting.

    SC79 purchased from MCE. Usage Cited in: Biomed Pharmacother. 2018 Oct;106:755-762.

    LTEP-a-2 cells are exposed to SC79 and GSK3β and AKT phosphorylation levels are significantly increased.

    SC79 purchased from MCE. Usage Cited in: Oncol Rep. 2019 Feb;41(2):811-818.

    Cells are cultured with and without CAR/SC79 for 24 h, and cell lysates are prepared and analyzed by western blotting to detect the levels of MDM2 and p53Ser15.

    SC79 purchased from MCE. Usage Cited in: Toxicology. 2019 May 1;419:32-39.

    HaCaT cells are treated with SC79 at 10 μM for 0, 0.5, 1, and 2 h, following arecoline treatment of 48 h. Proteins are extracted and used for Western blotting for phosphorylated and total Akt, mTOR, 4E-BP1, and cyclin D1. Akt, mTOR, 4E-BP1 and GAPDH are used as the internal control for p-Akt, p-mTOR, p-4E-BP1 and cyclin D1, respectively.

    SC79 purchased from MCE. Usage Cited in: Mol Med Rep. 2019 May;19(5):4091-4100.

    Western blot analysis demonstrating the protein levels of LC3, p62, AKT, p-AKT, mTOR and p-mTOR following treatment with Aβ1-42, 3-MA, SC79 and SC79 + RAPA for 48 h, and the expression of the AKT/mTOR signaling pathway‑associated proteins of AKT, p-AKT, mTOR and p-mTOR following treatment with Aβ1-42, 3-MA, SC79 and SC79 + RAPA for 48 h.

    View All Akt Isoform Specific Products:

    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    SC79 is a selective and cell-permeable Akt activator which activates Akt phosphorylation and inhibits Akt membrane translocation.

    IC50 & Target[1]

    Akt

     

    In Vitro

    SC79 reduces neuronal excitotoxicity and prevents stroke-induced neuronal death. SC79 suppresses PHAKTM-GFP plasma membrane translocation, and enhances phosphorylation of all three Akt isoforms in HEK293, HeLa, HL60, NB4, and HsSulton (B cells) cells[1]. SC79 restores proliferation of BRAT1 knockdown cells, and reduces the production of superoxide in mitochondria of MitoSox positive cells[2].

    In Vivo

    SC79 (0.04 mg/g, i.p.) inhibits the cytosolic activation of Akt, and recapitulates the primary cellular function of Akt signaling, resulting in augmented neuronal survival, in the permanent focal cerebral ischemia mouse model[1].

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (274.14 mM)

    H2O : < 0.1 mg/mL (insoluble)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.7414 mL 13.7069 mL 27.4138 mL
    5 mM 0.5483 mL 2.7414 mL 5.4828 mL
    10 mM 0.2741 mL 1.3707 mL 2.7414 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (6.85 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (6.85 mM); Clear solution

    References
    Cell Assay
    [1]

    HsSultan or NB4 cells (2.5×105) are plated in a 24-well plate in 500 μL of phenol red-free RPMI medium supplemented with 10% FBS. After incubation for 24 hours, each compound (8 µg/mL) is added and cultured for overnight (16-20 h). Fifty μLs of MTT solution (5 mg/mL in PBS) are added to each well. Following 2 hrs incubation, the purple formazan crystals are dissolved by directly adding in 500 μL of isopropanol with 0.1mol/LHCl to each well. After clearing the cell debris by centrifugation, the absorbance is measured at a wavelength of 570 nm.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [1]

    The permanent focal cerebral ischemia is induced by middle cerebral artery occlusion (MCAO) essentially. Briefly, mice (C57 Black/6) weighing 17-25 g are anesthetized with 4% isoflurane/66% N2O/30% O2 and maintained with 1.5% isoflurane. Permanent focal ischemia is achieved as follows: a 2-mm hole is drilled at a site superior and lateral to the left foramen ovale to expose the left middle cerebral artery. The proximal portion of the left middle cerebral artery (MCA) is permanently occluded over a 1-mm segment distal to the origin of the lenticulostriate branches through the use of a bipolar coagulator. SC79 is injected intraperitoneally (0.04 mg/g mouse body weight) 5 min before permanent MCAO. In another experiment, extra SC79 is injected (0.04 mg/g mouse body weight, once per hour for 6 hours).

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    364.78

    Formula

    C₁₇H₁₇ClN₂O₅

    CAS No.

    305834-79-1

    SMILES

    O=C(OCC)C(C#N)C1C(C(OCC)=O)=C(N)OC2=CC=C(Cl)C=C12

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

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    Product Name:
    SC79
    Cat. No.:
    HY-18749
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    SC79

    Cat. No.: HY-18749