2. Autophagy Apoptosis Cell Cycle/DNA Damage
  3. Autophagy Apoptosis Deubiquitinase
  4. Spautin-1

Spautin-1 is a specific and potent autophagy inhibitor which inhibits ubiquitin-specific peptidases, USP10 and USP13 with IC50s of 0.6-0.7 μM.

For research use only. We do not sell to patients.

CAS No. : 1262888-28-7

Size Price Stock Quantity
Free Sample (0.1 - 0.2 mg)   Apply Now  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 24 publication(s) in Google Scholar

Spautin-1 Related Antibodies

Top Publications Citing Use of Products

    Spautin-1 purchased from MedChemExpress. Usage Cited in: ACS Nano. 2025 Feb 11;19(5):5659-5679.  [Abstract]

    TAK-243/Spautin-1 groups results consistently support a positive correlation between the activity of HSP90, ubiquitination, and proteasome system with the disassembly of NSGs.

    Spautin-1 purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Sep 26;11(39):eadx3827.  [Abstract]

    HEK293T cells transfected with the indicated plasmid combinations were treated with Spautin-1 (10 μM) for 6 hours, Before collection, cells were treated with MG132 for 6 hours. Immunoblot analysis of NLRP3 ubiquitination in cell lysates immunoprecipitated with anti–c-Myc beads.

    Spautin-1 purchased from MedChemExpress. Usage Cited in: Cancer Immunol Immunother. 2025 Aug 25;74(9):293.  [Abstract]

    Cell viability of SU-DHL-4, SU-DHL-6, OCI-LY3, OCI-Ly10, and A20 cell lines detected by a CCK-8 kit after Spautin-1 (0.1, 0.3, 1, 3, 10, 30, 100 μM, 24, 48 h) treatment for the indicated time.

    Spautin-1 purchased from MedChemExpress. Usage Cited in: Cancer Immunol Immunother. 2025 Aug 25;74(9):293.  [Abstract]

    H&E staining showed that Spautin-1 (20 mg/kg, i.p.) induced tumor cell shrinkage, fragmentation, and disorganized tissue architecture.

    Spautin-1 purchased from MedChemExpress. Usage Cited in: Cancer Immunol Immunother. 2025 Aug 25;74(9):293.  [Abstract]

    Spautin-1 Spautin-1 (20 mg/kg, i.p.) treatment induced the infiltration of CD3+/CD4+ and CD8+ T cells into the tumor tissues.

    Spautin-1 purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2023 Feb;30(2):527-543.  [Abstract]

    ESCC cells were treated with different concentrations of Spautin-1 (0, 20, 40, 80 μM) for 24 h, and then western blotting was used to detect the protein levels of ANLN and USP10.

    View All Deubiquitinase Isoform Specific Products:

    View All Isoforms
    USP1 USP2 USP4 USP7 USP8 USP10 USP21 USP30 UCHL1 USP11 USP15

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Spautin-1 is a specific and potent autophagy inhibitor which inhibits ubiquitin-specific peptidases, USP10 and USP13 with IC50s of 0.6-0.7 μM.

    IC50 & Target

    USP10

    0.6-0.7 μM (IC50)

    Cellular Effect
    Cell Line Type Value Description References
    MIA PaCa-2 EC50
    20 μM
    Compound: Spautin-1
    Antiproliferative activity against human MIA PaCa-2 cells assessed as cell viability measured after 72 hrs by MTT assay
    Antiproliferative activity against human MIA PaCa-2 cells assessed as cell viability measured after 72 hrs by MTT assay
    		[PMID: 28105281]
    PC-9 IC50
    > 5 μM
    Compound: 5
    Cytotoxicity against human PC-9 cells harboring EGFR mutant assessed as inhibition of cell growth incubated for 72 hrs by ATP-based luminescence CellTiter-Glo assay
    Cytotoxicity against human PC-9 cells harboring EGFR mutant assessed as inhibition of cell growth incubated for 72 hrs by ATP-based luminescence CellTiter-Glo assay
    		[PMID: 36410591]
    In Vitro

    Spautin-1 enhances imatinib mesylate (IM)-induced CmL cell apoptosis by reducing the expression of the anti-apoptotic proteins Mcl-1 and Bcl-2. The pro-apoptotic activity of spautin-1 is associated with activation of GSK3β, an important downstream effector of PI3K/AKT. Spautin-1 enhances IM-induced cytotoxicity in CmL cell line K562, decreasing the IC50 from 1 to 0.5 μM[1]. The mechanism of spautin-1 acting on acute pancreatitis is associated with impaired autophagy inhibition[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Spautin-1 Related Antibodies
    In Vivo

    Spautin-1 ameliorates the pathogenesis of acute pancreatitis induced by cerulein or L-arginine. Spautin-1 pretreatment significantly diminishes the elevation of serum amylase and lipase levels, which are indicative of trypsin activity. Increasing levels of serum TNFα caused by cerulein are inhibited in the presence of spautin-1. Spautin-1 treatment can ameliorate the inflammation damage induced by cerulein, such as edema, degeneration, coagulative necrosis and infiltration of inflammatory cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    271.26

    Formula

    C15H11F2N3
    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    FC1=CC2=C(N=CN=C2C=C1)NCC3=CC=C(F)C=C3
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (184.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.6865 mL 18.4325 mL 36.8650 mL
    5 mM 0.7373 mL 3.6865 mL 7.3730 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (9.22 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (9.22 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG300    50% PBS

      Solubility: 10 mg/mL (36.87 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References
    Cell Assay
    [1]

    Spautin-1 is dissolved in DMSO. Cell proliferation is evaluated using CCK-8 kit. K562 cells (1x105/mL) are seeded into 96-well plates in triplicate and then treated with 125 to 4,000 nM IM alone or in combi¬nation with spautin-1 (10 μM). After 48 h of incubation, 10 μL of CCK-8 reagent is added to each well. Four hours later, the absorbance is read at 450 nm using a microplate reader[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [2]

    Mice: In this study, mice models with acute pancreatitis, including cerulein- and L-arginine-induced models, are constructed. For the cerulein-induced model, four intraperitoneal injections of cerulein (50 μg/kg body weight) are given consecutively at hourly intervals; The L-arginine-induced model received hourly intraperitoneal injections of 1.4 g/kg (optimal dosage for this study) L-arginine three times[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.6865 mL 18.4325 mL 36.8650 mL 92.1625 mL
    5 mM 0.7373 mL 3.6865 mL 7.3730 mL 18.4325 mL
    10 mM 0.3686 mL 1.8432 mL 3.6865 mL 9.2162 mL
    15 mM 0.2458 mL 1.2288 mL 2.4577 mL 6.1442 mL
    20 mM 0.1843 mL 0.9216 mL 1.8432 mL 4.6081 mL
    25 mM 0.1475 mL 0.7373 mL 1.4746 mL 3.6865 mL
    30 mM 0.1229 mL 0.6144 mL 1.2288 mL 3.0721 mL
    40 mM 0.0922 mL 0.4608 mL 0.9216 mL 2.3041 mL
    50 mM 0.0737 mL 0.3686 mL 0.7373 mL 1.8432 mL
    60 mM 0.0614 mL 0.3072 mL 0.6144 mL 1.5360 mL
    80 mM 0.0461 mL 0.2304 mL 0.4608 mL 1.1520 mL
    100 mM 0.0369 mL 0.1843 mL 0.3686 mL 0.9216 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Spautin-1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Spautin-11262888-28-7Spautin1Spautin 1AutophagyApoptosisDeubiquitinaseDUBsInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Spautin-1
    Cat. No.:
    HY-12990
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (476 KB) Français - FR (476 KB) Deutsch - DE (476 KB) Norwegian - NO (476 KB) Español - ES (476 KB) Swedish - SV (476 KB) Italian - IT (476 KB) Korean - KR (476 KB) Portuguese - PT (476 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.